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GRAMD2A (NM_001012642) Human Untagged Clone

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SKU
SC301686
GRAMD2 (untagged)-Human GRAM domain containing 2 (GRAMD2)
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GRAMD2A
Synonyms GRAMD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC301686 representing NM_001012642.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGACCGCTTTAAGCCGGAGCGAGGCCACCGAGGAGGGCGGCAACCAACAAATGCACAGAAAGACAGCT
TCTCTGAACAGTCCTGTGTCCTGCAAAGAGAAACCAGACAGAGTTGAGGAGCCCCCGGACTACAGTCTG
CACTGGCCAGAAGGCTTGAAGGGTGAAGAGATAAAGAAGTGTGGCCGAGAAGGGATAACACTGAATAAA
TACAACCAGCAATACCACAAGCTGTTTAAGGATGTTCCCTTGGAGGAAGTGGTTCTCAAAGTGTGTTCC
TGTGCCCTCCAGAGGGACTTCCTCCTCCAGGGCCGGCTCTACATCTCCCCCAACTGGCTCTGCTTCCAT
GCCAGCCTCTTTGGCAAGGATATCAAGGTGGTCATTCCTGTGGTGTCTGTGCAAATGATCAAAAAACAC
AAGATGGCACGGCTCCTTCCCAATGGACTGGCCATCACCACCAACACCAGCCAGAAGTATATCTTTGTG
TCACTGCTCTCCCGGGACAGTGTATATGACCTGCTGAGGAGAGTCTGCACCCACCTACAGCCTTCCAGC
AAGAAGAGTCTGAGTGTAAGAGAATTTTCAGGGGAACCTGAGTCTCTGGAAGTCCTCATCCCTGAGATG
AAGTGGAGAAAGGTATGCCCTTCCTCCAGGTCCCTGTCTCTCCCAGACAACATCCCTTGTATCCCTCCA
TCATCCGTGGACTCCACAGACAGTTTCTTCCCCTCCAGGAAGCCTCCAATGTCTGAAAAGTCAAGAGCC
CAAGTAGCTTCAGAAAATGGTGGGAGGTGGGCATGGCCCATGCCAGGCTGGGGTCCTGCCTGCCCTAAG
AAGATGCCGAACTGCTCTCCCACTGCAAAGAATGCTGTCTATGAGGAGGATGAGCTGGAGGAGGAGCCC
AGGAGCACTGGGGAGCTGAGGCTCTGGGATTACCGGCTCCTCAAGGTCTTCTTTGTGCTGATCTGCTTC
CTGGTCATGTCCTCATCCTACCTGGCGTTCCGTATTTCTCGGCTAGAGCAGCAGTTATGCTCCTTGAGT
TGGGATGACCCAGTCCCTGGGCACAGGTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001012642
Insert Size 1065 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001012642.2
RefSeq Size 3318 bp
RefSeq ORF 1065 bp
Locus ID 196996
UniProt ID Q8IUY3
Cytogenetics 15q23
Protein Families Transmembrane
MW 40.2 kDa
Summary Participates in the organization of endoplasmic reticulum-plasma membrane contact sites (EPCS) with pleiotropic functions including STIM1 recruitment and calcium homeostasis. Constitutive tether that co-localize with ESYT2/3 tethers at endoplasmic reticulum-plasma membrane contact sites in a phosphatidylinositol lipid-dependent manner. Pre-marks the subset of phosphtidylinositol 4,5-biphosphate (PI(4,5)P2)-enriched EPCS destined for the store operated calcium entry pathway (SOCE).UniProtKB/Swiss-Prot Function
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"NM_001012642" in other vectors (4)
SKU Description Size Price
RC220145 GRAMD2 (Myc-DDK-tagged)-Human GRAM domain containing 2 (GRAMD2) 10 ug
$457.00
RC220145L3 Lenti ORF clone of Human GRAM domain containing 2 (GRAMD2), Myc-DDK-tagged 10 ug
$757.00
RC220145L4 Lenti ORF clone of Human GRAM domain containing 2 (GRAMD2), mGFP tagged 10 ug
$757.00
RG220145 GRAMD2 (tGFP-tagged) - Human GRAM domain containing 2 (GRAMD2) 10 ug
$657.00

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