GRAMD2 (GRAMD2A) (NM_001012642) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC220145L4
Lenti ORF clone of Human GRAM domain containing 2 (GRAMD2), mGFP tagged
  $757.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GRAMD2A
Synonyms GRAMD2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC220145).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001012642
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001012642.1
RefSeq Size 3280 bp
RefSeq ORF 1065 bp
Locus ID 196996
UniProt ID Q8IUY3
Cytogenetics 15q23
Protein Families Transmembrane
MW 40.1 kDa
Summary Participates in the organization of endoplasmic reticulum-plasma membrane contact sites (EPCS) with pleiotropic functions including STIM1 recruitment and calcium homeostasis. Constitutive tether that co-localize with ESYT2/3 tethers at endoplasmic reticulum-plasma membrane contact sites in a phosphatidylinositol lipid-dependent manner. Pre-marks the subset of phosphtidylinositol 4,5-biphosphate (PI(4,5)P2)-enriched EPCS destined for the store operated calcium entry pathway (SOCE).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001012642" in other vectors (4)
SKU Description Size Price
RC220145 GRAMD2 (Myc-DDK-tagged)-Human GRAM domain containing 2 (GRAMD2) 10 ug
$457.00
RC220145L3 Lenti ORF clone of Human GRAM domain containing 2 (GRAMD2), Myc-DDK-tagged 10 ug
$757.00
RG220145 GRAMD2 (tGFP-tagged) - Human GRAM domain containing 2 (GRAMD2) 10 ug
$657.00
SC301686 GRAMD2 (untagged)-Human GRAM domain containing 2 (GRAMD2) 10 ug
$503.00

Plasmid Map for RC220145L4

Download sequence for

Cloning scheme for RC220145L4

Circular map for RC220145L4

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products