BD2 (DEFB4A) (NM_004942) Human Tagged ORF Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BD2 |
| Synonyms | BD-2; DEFB-2; DEFB2; DEFB4; DEFB102; HBD-2; SAP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004942 |
| ORF Size | 192 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004942.4 |
| RefSeq Size | 460 bp |
| RefSeq ORF | 195 bp |
| Locus ID | 1673 |
| UniProt ID | O15263 |
| Cytogenetics | 8p23.1 |
| Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
| MW | 7 kDa |
| Summary | Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004942" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC219487L1 | Lenti ORF clone of Human defensin, beta 4A (DEFB4A), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC219487L2 | Lenti ORF clone of Human defensin, beta 4A (DEFB4A), mGFP tagged | 10 ug |
$450.00
|
|
| RC219487L3 | Lenti ORF clone of Human defensin, beta 4A (DEFB4A), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC219487L4 | Lenti ORF clone of Human defensin, beta 4A (DEFB4A), mGFP tagged | 10 ug |
$450.00
|
|
| RG219487 | DEFB4A (tGFP-tagged) - Human defensin, beta 4A (DEFB4A) | 10 ug |
$489.00
|
|
| SC303551 | DEFB4A (untagged)-Human defensin, beta 4A (DEFB4A) | 10 ug |
$150.00
|
|
Citations
IL-1β-Induced Protection of Keratinocytes against Staphylococcus aureus-Secreted Proteases Is Mediated by Human β-Defensin 2
Wang, McHugh, Qureshi et al
J. Invest. Dermatol. (2017) 137 (1), 95-105 DOI: 10.1016/j.jid.2016.08.025
Wang, McHugh, Qureshi et al
J. Invest. Dermatol. (2017) 137 (1), 95-105 DOI: 10.1016/j.jid.2016.08.025
AI Summary: OriGene's TrueORF Gold pCMV6-DEFB4 gene expression clone and pCMV6 vector backbone were used to generate keratinocyte cell lines overexpressing human β-defensin 2 or serving as controls, enabling the study of hBD2's protective role against Staphylococcus aureus protease-induced barrier damage.
Snippet: To create hBD2 OE and VO control HaCaT lines, True ORF Gold pCMV6-DEFB4 gene expression clone or pCMV6 vector o nly backbone (OriGene Technologies, U.S.A.) were transfected with Lipofec tamine 2000 (Life Technologies, Renfrew, U.K.), followed by selection with 500μ g/ml G418 (Life Technologies)
IL-1-Induced Protection of Keratinocytes against Staphylococcus aureus-Secreted Proteases Is Mediated by Human -Defensin 2
Wang, McHugh, Qureshi et al
Journal of Investigative Dermatology (2016)
Wang, McHugh, Qureshi et al
Journal of Investigative Dermatology (2016)
AI Summary: OriGene products, including the TrueORF Gold pCMV6-DEFB4 gene expression clone and plasmids for DEFB1 (#SC116851) and DEFB4 (#RC219487), were used to generate and validate overexpression of human β-defensin 2 in keratinocyte cell lines for this study.
Snippet: To create hBD2 OE and VO control HaCaT lines, TrueORF Gold pCMV6-DEFB4 gene expression clone or pCMV6 vector only backbone (OriGene Technologies, U.S.A.) were transfected with Lipofectamine 2000 (Life Technologies, Renfrew, U.K.), followed by selection with 500µg/ml G418 (Life Technologies). Standard curves were performed using plasmids for DEFB1, DEFB4 (Origene #SC116851, RC219487), and DEFB103 (generated by J. …
Showing 1-2 of 2 papers.
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