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ZWILCH (NM_017975) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC213970
ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $550.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ZWILCH or NM_017975"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZWILCH
Synonyms hZwilch; KNTC1AP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213970 representing NM_017975
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCCC

ATGTGGGAGCGGCTGAACTGCGCAGCAGAGGACTTTTATTCTCGTCTCCTTCAGAAATTTAATGAAGAAA
AGAAAGGAATCCGTAAAGACCCATTTCTCTATGAGGCTGATGTCCAAGTGCAGTTGATCAGCAAAGGCCA
ACCAAACCCTTTGAAAAATATTCTAAATGAAAATGACATAGTATTCATAGTGGAAAAAGTGCCTTTAGAA
AAGGAAGAAACAAGTCATATTGAAGAACTTCAATCTGAAGAAACTGCCATATCTGATTTCTCTACTGGCG
AAAATGTTGGACCACTTGCTTTACCAGTTGGGAAGGCAAGGCAGTTAATTGGACTTTACACCATGGCTCA
CAATCCTAATATGACCCATTTGAAGATTAATCTGCCAGTTACTGCCCTTCCTCCCCTTTGGGTAAGATGT
GACAGTTCAGATCCTGAAGGTACTTGTTGGCTAGGAGCTGAGCTTATCACAACAAACAACAGCATTACAG
GAATTGTCTTATATGTGGTCAGTTGTAAAGCTGATAAAAATTATTCTGTAAATCTTGAAAACCTAAAAAA
TTTACACAAGAAAAGACATCACTTGTCTACTGTAACATCCAAAGGCTTTGCCCAGTATGAGCTCTTTAAG
TCCTCTGCCTTGGATGATACAATCACAGCATCACAAACTGCGATCGCTTTGGATATTTCCTGGAGTCCTG
TGGATGAGATTCTTCAAATCCCTCCACTCTCTTCAACTGCAACTCTGAATATTAAAGTGGAATCAGGAGA
GCCCAGAGGTCCTTTGAATCATCTCTACAGAGAACTGAAATTTCTTCTTGTTTTGGCTGATGGTTTGAGG
ACTGGTGTCACTGAATGGCTCGAGCCCCTGGAAGCAAAATCTGCTGTTGAACTTGTTCAGGAATTTCTGA
ATGACTTAAATAAGCTGGATGGATTTGGTGATTCTACAAAAAAAGACACTGAGGTTGAGACCTTGAAGCA
TGACACTGCTGCAGTCGATCGTTCCGTCAAGCGTCTTTTCAAAGTTCGGAGTGATCTTGATTTTGCTGAG
CAACTGTGGTGCAAAATGAGCAGTAGTGTGATTTCATACCAAGACTTGGTGAAGTGTTTCACATTGATCA
TCCAGAGTCTACAACGTGGTGATATACAGCCATGGCTCCATAGTGGAAGTAACAGTTTACTAAGTAAGCT
CATTCATCAGTCTTATCATGGAACCATGGACACAGTTTCTCTCAGTGGGACTATTCCAGTTCAAATGCTT
TTGGAAATTGGTTTGGACAAACTAAAGAAAGATTATATCAGTTTTTTCATAGGTCAGGAACTTGCATCTT
TGAATCATTTGGAATACTTCATTGCTCCATCAGTAGATATACAAGAACAGGTTTATCGTGTCCAAAAACT
CCACCATATTCTAGAAATATTAGTCAGTTGCATGCCTTTCATTAAATCTCAACATGAACTCCTCTTTTCT
TTAACACAGATCTGCATAAAGTATTACAAACAAAATCCTCTTGATGAGCAACACATTTTTCAGCTGCCAG
TCAGACCAACTGCTGTAAAGAACTTATATCAAAGTGAGAAGCCACAGAAATGGAGAGTGGAAATATATAG
TGGTCAAAAGAAGATTAAGACAGTTTGGCAACTGAGTGACAGCTCACCCATAGACCATCTGAATTTTCAC
AAACCTGATTTTTCGGAATTAACACTAAACGGTAGCCTGGAAGAAAGGATATTCTTTACTAACATGGTTA
CCTGCAGCCAGGTGCATTTCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC213970 representing NM_017975
Red=Cloning site Green=Tags(s)

MWERLNCAAEDFYSRLLQKFNEEKKGIRKDPFLYEADVQVQLISKGQPNPLKNILNENDIVFIVEKVPLE
KEETSHIEELQSEETAISDFSTGENVGPLALPVGKARQLIGLYTMAHNPNMTHLKINLPVTALPPLWVRC
DSSDPEGTCWLGAELITTNNSITGIVLYVVSCKADKNYSVNLENLKNLHKKRHHLSTVTSKGFAQYELFK
SSALDDTITASQTAIALDISWSPVDEILQIPPLSSTATLNIKVESGEPRGPLNHLYRELKFLLVLADGLR
TGVTEWLEPLEAKSAVELVQEFLNDLNKLDGFGDSTKKDTEVETLKHDTAAVDRSVKRLFKVRSDLDFAE
QLWCKMSSSVISYQDLVKCFTLIIQSLQRGDIQPWLHSGSNSLLSKLIHQSYHGTMDTVSLSGTIPVQML
LEIGLDKLKKDYISFFIGQELASLNHLEYFIAPSVDIQEQVYRVQKLHHILEILVSCMPFIKSQHELLFS
LTQICIKYYKQNPLDEQHIFQLPVRPTAVKNLYQSEKPQKWRVEIYSGQKKIKTVWQLSDSSPIDHLNFH
KPDFSELTLNGSLEERIFFTNMVTCSQVHFK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017975
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017975.5
RefSeq Size 3204 bp
RefSeq ORF 1776 bp
Locus ID 55055
UniProt ID Q9H900
Cytogenetics 15q22.31
MW 67.7 kDa
Summary Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).UniProtKB/Swiss-Prot Function
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"NM_017975" in other vectors (5)
SKU Description Size Price
RC213970L3 Lenti-ORF clone of ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$850.00
RC213970L4 Lenti-ORF clone of ZWILCH (mGFP-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$850.00
RG213970 ZWILCH (tGFP-tagged) - Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$750.00
SC113827 ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$150.00
SC318018 ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$1,023.00

Plasmid Map for RC213970

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC213970
Circular map for RC213970

file-alt Citations

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