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ZWILCH (NM_017975) Human Untagged Clone

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SKU
SC113827
ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1
  $150.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ZWILCH
Synonyms hZwilch; KNTC1AP
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113827 sequence for NM_017975 edited (data generated by NextGen Sequencing)
ATGTGGGAGCGGCTGAACTGCGCAGCAGAGGACTTTTATTCTCGTCTCCTTCAGAAATTT
AATGAAGAAAAGAAAGGAATCCGTAAAGACCCATTTCTCTATGAGGCTGATGTCCAAGTG
CAGTTGATCAGCAAAGGCCAACCAAACCCTTTGAAAAATATTCTAAATGAAAATGACATA
GTATTCATAGTGGAAAAAGTGCCTTTAGAAAAGGAAGAAACAAGTCATATTGAAGAACTT
CAATCTGAAGAAACTGCCATATCTGATTTCTCTACTGGCGAAAATGTTGGACCACTTGCT
TTACCAGTTGGGAAGGCAAGGCAGTTAATTGGACTTTACACCATGGCTCACAATCCTAAT
ATGACCCATTTGAAGATTAATCTGCCAGTTACTGCCCTTCCTCCCCTTTGGGTAAGATGT
GACAGTTCAGATCCTGAAGGTACTTGTTGGCTAGGAGCTGAGCTTATCACAACAAACAAC
AGCATTACAGGAATTGTCTTATATGTGGTCAGTTGTAAAGCTGATAAAAATTATTCTGTA
AATCTTGAAAACCTAAAAAATTTACACAAGAAAAGACATCACTTGTCTACTGTAACATCC
AAAGGCTTTGCCCAGTATGAGCTCTTTAAGTCCTCTGCCTTGGATGATACAATCACAGCA
TCACAAACTGCGATCGCTTTGGATATTTCCTGGAGTCCTGTGGATGAGATTCTTCAAATC
CCTCCACTCTCTTCAACTGCAACTCTGAATATTAAAGTGGAATCAGGAGAGCCCAGAGGT
CCTTTGAATCATCTCTACAGAGAACTGAAATTTCTTCTTGTTTTGGCTGATGGTTTGAGG
ACTGGTGTCACTGAATGGCTCGAGCCCCTGGAAGCAAAATCTGCTGTTGAACTTGTTCAG
GAATTTCTGAATGACTTAAATAAGCTGGATGGATTTGGTGATTCTACAAAAAAAGACACT
GAGGTTGAGACCTTGAAGCATGACACTGCTGCAGTCGATCGTTCCGTCAAGCGTCTTTTC
AAAGTTCGGAGTGATCTTGATTTTGCTGAGCAACTGTGGTGCAAAATGAGCAGTAGTGTG
ATTTCATACCAAGACTTGGTGAAGTGTTTCACATTGATCATCCAGAGTCTACAACGTGGT
GATATACAGCCATGGCTCCATAGTGGAAGTAACAGTTTACTAAGTAAGCTCATTCATCAG
TCTTATCATGGAACCATGGACACAGTTTCTCTCAGTGGGACTATTCCAGTTCAAATGCTT
TTGGAAATTGGTTTGGACAAACTAAAGAAAGATTATATCAGTTTTTTCATAGGTCAGGAA
CTTGCATCTTTGAATCATTTGGAATACTTCATTGCTCCATCAGTAGATATACAAGAACAG
GTTTATCGTGTCCAAAAACTCCACCATATTCTAGAAATATTAGTCAGTTGCATGCCTTTC
ATTAAATCTCAACATGAACTCCTCTTTTCTTTAACACAGATCTGCATAAAGTATTACAAA
CAAAATCCTCTTGATGAGCAACACATTTTTCAGCTGCCAGTCAGACCAACTGCTGTAAAG
AACTTATATCAAAGTGAGAAGCCACAGAAATGGAGAGTGGAAATATATAGTGGTCAAAAG
AAGATTAAGACAGTTTGGCAACTGAGTGACAGCTCACCCATAGACCATCTGAATTTTCAC
AAACCTGATTTTTCGGAATTAACACTAAACGGTAGCCTGGAAGAAAGGATATTCTTTACT
AACATGGTTACCTGCAGCCAGGTGCATTTCAAGTGA

Clone variation with respect to NM_017975.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_017975 unedited
TACCCGACTTCTGTGGGGCGGAAAACCATTCGGCACGAGGGGGATGTGGGATCGGCTGAA
CTGCGCAGCAGAGGACTCTTATTCTCGTCTCCTTCACCTTTAGAAAAGGAAGAAACAAGT
CATATTGAAAAACTTCAATCTGAAGAAACTGCCATATCTGATTTCTCTACTGGCGAAAAT
GTTGGACCACTTGCTTTACCATTCGTGATTGCAAGGCAGTTAATTGGACTTTACACCATG
GCTCACAATCCTAATATGACCCATTTGAAGATTAATCTGCCAGTTACTGCCCTTCCTCCC
CTTTGGGTAAGATGTGACAGTTCAGATCCTGAAGGTACTTGTTGGCTAGGAGCTGAGCTT
ATCACAACAAACAACAGCATTACAGGAATTGTCTTATATGTGGTCAGTTGTAAAGCTGAT
AAAAATTATTCTGTAAATCTTGAAAACCTAAAAAATTTACACAAGAAAAGACATCACTTG
TCTACTGTAACATCCAAAGGCTTTGCCCAGTATGAGCTCTTTAAGTCCTCTGCCTTGGAT
GATACAATCACAGCATCACAAACTGCGATCGCTTTGGATATTTCCTGGAGTCCTGTGGAT
GAGATTCTTCANATCCCTCCACTCTCTTCAACTGCAACTCTGAATATTAAAGTGGAATCA
GGAGAGCCCAGAGGTCCTTTTGATCATCTCTACAGAGAACTGANATTTCTTCTTGTTTTG
GCTGATGGTTTGAGGACTGGTGTCACTGAATGCCTCGAGCCCCTGGAAGCAAATCTGCTG
TTGAACTTGTTCAGAATTCTGATGACTTAAATAGCTGAATGGATTTGTGATTCTACAAAA
AGAACTGAGGTGAAACTTGAGCATGACCTGCTGCAGTCACGTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_017975 unedited
CTCTCTTTATGTGGTAGCAAANATGGAAGTGCGAGACCAAATCTTACCAAACTAGCTATT
TTTACAGGCCAATAAAGCAACATGCAATCCCCCTCAACAAATTTAAATAATCAGGCAATA
CTAAGAATGTATATTCCATTAAACTAAAATAAACAAGGTTGAAATGTGGTACAGAATTCA
CTGATGAGCCTGTGAACTCCACGTGAGGATGTCCAGTGCCTTATTTATCTCAGTAACCAG
AGTACCCAGCACACAAGATAAAAGTGGGTATTACCTAAGTGGCCACTATTTTATTAATAA
TGCACATAACATATGCTTATCATTAACTCTTAAAAAGATTATTATTTAACTATACAAGAA
CTACCATACAACATTTCAACATACAAGTTCCTATACTTCTTAAGATACACAATAATATTT
TGAAAGATTGGAAATGTTTCCCAAGGACTTTCCTATCACAAATCCCCCCCAGTAAAAATT
ATAGGAACTGTGGAATATGGCTGCTAATATTCTGAGATGAATTTAGAAAGTATAAGTATT
GAGAGATGGACTTTATTATAGCACCTGTAGAAGAACCAAATTCAGGCATTTATCCCATAT
TCTCTGAAATATTTACAAAGCTCTTATAACATTTTCAAATAACATTTAATTTACTAAGTT
CTATTTCATGGCAAATAAGAATCAGAAAATTTGAGGATGCTATTTTTTAAAGTTTCCCAA
TACAGTTAAAGCTTTTGGGATTAAGCTGTATTTACTAAAACGATCCCTGTCTCATTTGTG
GCGCCCGTTCTCTTTGTCATCCCGAGCCCTTGCTCAGCACCATGCAATGGTACTGAAATC
GTTGGACCCACTGGCTGCGTCCTCTGTGACTCAAACTGCTGTCTATTAAGCTCCTCCTGA
GAAGCATAGCTAAACAGGATTTCATATCTGGAGTCTCCAAGAAAAACTCCAGTTCTTTCT
CCCTTTTCTGGTTGAGGGT
Restriction Sites NotI-NotI |
ACCN NM_017975
Insert Size 2930 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017975.1, NP_060445.1
RefSeq Size 1670 bp
RefSeq ORF 405 bp
Locus ID 55055
UniProt ID Q9H900
Cytogenetics 15q22.31
Summary Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_017975" in other vectors (5)
SKU Description Size Price
RC213970 ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$289.00 $550.00
RC213970L3 Lenti-ORF clone of ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$850.00
RC213970L4 Lenti-ORF clone of ZWILCH (mGFP-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$850.00
RG213970 ZWILCH (tGFP-tagged) - Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$750.00
SC318018 ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$1,023.00

file-alt Citations

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