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ZWILCH (NM_017975) Human Untagged Clone

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SKU
SC318018
ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1
  $1,023.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ZWILCH
Synonyms hZwilch; KNTC1AP
Vector pCMV6 series
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_017975, the custom clone sequence may differ by one or more nucleotides
ATGTGGGAGCGGCTGAACTGCGCAGCAGAGGACTTTTATTCTCGTCTCCTTCAGAAATTT
AATGAAGAAAAGAAAGGAATCCGTAAAGACCCATTTCTCTATGAGGCTGATGTCCAAGTG
CAGTTGATCAGCAAAGGCCAACCAAACCCTTTGAAAAATATTCTAAATGAAAATGACATA
GTATTCATAGTGGAAAAAGTGCCTTTAGAAAAGGAAGAAACAAGTCATATTGAAGAACTT
CAATCTGAAGAAACTGCCATATCTGATTTCTCTACTGGCGAAAATGTTGGACCACTTGCT
TTACCAGTTGGGAAGGCAAGGCAGTTAATTGGACTTTACACCATGGCTCACAATCCTAAT
ATGACCCATTTGAAGATTAATCTGCCAGTTACTGCCCTTCCTCCCCTTTGGGTAAGATGT
GACAGTTCAGATCCTGAAGGTACTTGTTGGCTAGGAGCTGAGCTTATCACAACAAACAAC
AGCATTACAGGAATTGTCTTATATGTGGTCAGTTGTAAAGCTGATAAAAATTATTCTGTA
AATCTTGAAAACCTAAAAAATTTACACAAGAAAAGACATCACTTGTCTACTGTAACATCC
AAAGGCTTTGCCCAGTATGAGCTCTTTAAGTCCTCTGCCTTGGATGATACAATCACAGCA
TCACAAACTGCGATCGCTTTGGATATTTCCTGGAGTCCTGTGGATGAGATTCTTCAAATC
CCTCCACTCTCTTCAACTGCAACTCTGAATATTAAAGTGGAATCAGGAGAGCCCAGAGGT
CCTTTGAATCATCTCTACAGAGAACTGAAATTTCTTCTTGTTTTGGCTGATGGTTTGAGG
ACTGGTGTCACTGAATGGCTCGAGCCCCTGGAAGCAAAATCTGCTGTTGAACTTGTTCAG
GAATTTCTGAATGACTTAAATAAGCTGGATGGATTTGGTGATTCTACAAAAAAAGACACT
GAGGTTGAGACCTTGAAGCATGACACTGCTGCAGTCGATCGTTCCGTCAAGCGTCTTTTC
AAAGTTCGGAGTGATCTTGATTTTGCTGAGCAACTGTGGTGCAAAATGAGCAGTAGTGTG
ATTTCATACCAAGACTTGGTGAAGTGTTTCACATTGATCATCCAGAGTCTACAACGTGGT
GATATACAGCCATGGCTCCATAGTGGAAGTAACAGTTTACTAAGTAAGCTCATTCATCAG
TCTTATCATGGAACCATGGACACAGTTTCTCTCAGTGGGACTATTCCAGTTCAAATGCTT
TTGGAAATTGGTTTGGACAAACTAAAGAAAGATTATATCAGTTTTTTCATAGGTCAGGAA
CTTGCATCTTTGAATCATTTGGAATACTTCATTGCTCCATCAGTAGATATACAAGAACAG
GTTTATCGTGTCCAAAAACTCCACCATATTCTAGAAATATTAGTCAGTTGCATGCCTTTC
ATTAAATCTCAACATGAACTCCTCTTTTCTTTAACACAGATCTGCATAAAGTATTACAAA
CAAAATCCTCTTGATGAGCAACACATTTTTCAGCTGCCAGTCAGACCAACTGCTGTAAAG
AACTTATATCAAAGTGAGAAGCCACAGAAATGGAGAGTGGAAATATATAGTGGTCAAAAG
AAGATTAAGACAGTTTGGCAACTGAGTGACAGCTCACCCATAGACCATCTGAATTTTCAC
AAACCTGATTTTTCGGAATTAACACTAAACGGTAGCCTGGAAGAAAGGATATTCTTTACT
AACATGGTTACCTGCAGCCAGGTGCATTTCAAG
Restriction Sites Please inquire |
ACCN NM_017975
Insert Size 3204 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017975.3, NP_060445.3
RefSeq Size 3204 bp
RefSeq ORF 3204 bp
Locus ID 55055
UniProt ID Q9H900
Cytogenetics 15q22.31
Summary Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_017975" in other vectors (5)
SKU Description Size Price
RC213970 ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$289.00 $550.00
RC213970L3 Lenti-ORF clone of ZWILCH (Myc-DDK-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$850.00
RC213970L4 Lenti-ORF clone of ZWILCH (mGFP-tagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$850.00
RG213970 ZWILCH (tGFP-tagged) - Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$750.00
SC113827 ZWILCH (untagged)-Human Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), transcript variant 1 10 ug
$150.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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