CLNK (NM_052964) Human Tagged ORF Clone

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SKU
RC213867
CLNK (Myc-DDK-tagged)-Human cytokine-dependent hematopoietic cell linker (CLNK)
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CLNK
Synonyms MIST
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC213867 representing NM_052964
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGGCAGGGCAATAGAAAGACAACTAAAGAAGGATCCAACGATTTGAAATTCCAGAACTTCAGTC
TGCCAAAAAACAGGTCATGGCCTCGCATCAATAGTGCCACAGGCCAGTACCAGAGGATGAACAAGCCTCT
TCTAGACTGGGAAAGAAACTTTGCTGCAGTCCTGGATGGAGCAAAAGGCCACAGTGATGATGACTATGAT
GACCCTGAGCTTCGGATGGAAGAGACATGGCAGTCGATTAAAATTTTACCAGCCCGGCCTATAAAGGAAT
CTGAATATGCAGATACACACTATTTCAAGGTTGCAATGGACACTCCCCTTCCGTTAGACACCAGGACCTC
TATCTCCATTGGACAGCCGACCTGGAACACACAGACGAGGTTGGAAAGAGTGGACAAACCCATTTCCAAG
GACGTCAGAAGCCAAAACATTAAAGGAGATGCATCCGTAAGAAAGAACAAGATTCCTTTACCACCTCCTC
GGCCTCTCATAACACTTCCGAAGAAGTACCAACCCTTGCCCCCTGAGCCGGAGAGCAGCAGGCCACCTTT
ATCTCAGAGACACACCTTTCCAGAAGTCCAGAGAATGCCCAGTCAGATAAGCTTAAGGGACTTAAGTGAG
GTCCTTGAAGCAGAAAAAGTTCCTCATAACCAGAGGAAGCCTGAATCAACTCATCTGTTAGAAAACCAAA
ATACTCAAGAGATTCCACTTGCCATTAGCAGTTCTTCATTCACGACAAGCAACCACAGTGTGCAAAACAG
AGATCATAGAGGAGGCATGCAGCCCTGTTCTCCTCAGAGATGCCAGCCTCCAGCCAGCTGCAGCCCTCAC
GAAAATATACTGCCCTATAAATACACAAGCTGGAGACCACCTTTCCCCAAAAGGTCTGATAGAAAGGATG
TCCAGCACAATGAATGGTACATTGGAGAATACAGCCGCCAGGCAGTGGAAGAGGCATTCATGAAGGAGAA
CAAGGATGGTAGTTTCTTGGTCCGAGATTGTTCCACAAAATCCAAGGAAGAGCCCTATGTTTTGGCTGTG
TTTTATGAGAACAAAGTCTACAATGTAAAAATCCGCTTCCTGGAGAGGAATCAGCAGTTTGCCCTGGGGA
CAGGACTCAGAGGAGATGAGAAGTTTGATTCAGTAGAAGACATCATCGAACACTACAAGAATTTTCCCAT
TATACTAATTGATGGGAAAGATAAAACTGGGGTCCACAGGAAACAGTGTCACCTCACTCAGCCACTCCCT
CTCACCAGACACCTCTTGCCTCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC213867 representing NM_052964
Red=Cloning site Green=Tags(s)

MNRQGNRKTTKEGSNDLKFQNFSLPKNRSWPRINSATGQYQRMNKPLLDWERNFAAVLDGAKGHSDDDYD
DPELRMEETWQSIKILPARPIKESEYADTHYFKVAMDTPLPLDTRTSISIGQPTWNTQTRLERVDKPISK
DVRSQNIKGDASVRKNKIPLPPPRPLITLPKKYQPLPPEPESSRPPLSQRHTFPEVQRMPSQISLRDLSE
VLEAEKVPHNQRKPESTHLLENQNTQEIPLAISSSSFTTSNHSVQNRDHRGGMQPCSPQRCQPPASCSPH
ENILPYKYTSWRPPFPKRSDRKDVQHNEWYIGEYSRQAVEEAFMKENKDGSFLVRDCSTKSKEEPYVLAV
FYENKVYNVKIRFLERNQQFALGTGLRGDEKFDSVEDIIEHYKNFPIILIDGKDKTGVHRKQCHLTQPLP
LTRHLLPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052964
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052964.3
RefSeq Size 1814 bp
RefSeq ORF 1287 bp
Locus ID 116449
UniProt ID Q7Z7G1
Cytogenetics 4p16.1
MW 49.4 kDa
Summary MIST is a member of the SLP76 family of adaptors (see LCP2, MIM 601603; BLNK, MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling, including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A, MIM 147140)-mediated mast cell degranulation (Cao et al., 1999 PubMed 10562326; Goitsuka et al., 2000, 2001 PubMed 10744659 PubMed 11463797).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_052964" in other vectors (4)
SKU Description Size Price
RC213867L3 Lenti ORF clone of Human cytokine-dependent hematopoietic cell linker (CLNK), Myc-DDK-tagged 10 ug
$757.00
RC213867L4 Lenti ORF clone of Human cytokine-dependent hematopoietic cell linker (CLNK), mGFP tagged 10 ug
$757.00
RG213867 CLNK (tGFP-tagged) - Human cytokine-dependent hematopoietic cell linker (CLNK) 10 ug
$657.00
SC305708 CLNK (untagged)-Human cytokine-dependent hematopoietic cell linker (CLNK) 10 ug
$503.00

Plasmid Map for RC213867

Download sequence for

Cloning scheme for RC213867

Circular map for RC213867

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