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CLNK (NM_052964) Human Untagged Clone

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SKU
SC305708
CLNK (untagged)-Human cytokine-dependent hematopoietic cell linker (CLNK)
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CLNK
Synonyms MIST
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC305708 representing NM_052964.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAACAGGCAGGGCAATAGAAAGACAACTAAAGAAGGATCCAACGATTTGAAATTCCAGAACTTCAGT
CTGCCAAAAAACAGGTCATGGCCTCGCATCAATAGTGCCACAGGCCAGTACCAGAGGATGAACAAGCCT
CTTCTAGACTGGGAAAGAAACTTTGCTGCAGTCCTGGATGGAGCAAAAGGCCACAGTGATGATGACTAT
GATGACCCTGAGCTTCGGATGGAAGAGACATGGCAGTCGATTAAAATTTTACCAGCCCGGCCTATAAAG
GAATCTGAATATGCAGATACACACTATTTCAAGGTTGCAATGGACACTCCCCTTCCGTTAGACACCAGG
ACCTCTATCTCCATTGGACAGCCGACCTGGAACACACAGACGAGGTTGGAAAGAGTGGACAAACCCATT
TCCAAGGACGTCAGAAGCCAAAACATTAAAGGAGATGCATCCGTAAGAAAGAACAAGATTCCTTTACCA
CCTCCTCGGCCTCTCATAACACTTCCGAAGAAGTACCAACCCTTGCCCCCTGAGCCGGAGAGCAGCAGG
CCACCTTTATCTCAGAGACACACCTTTCCAGAAGTCCAGAGAATGCCCAGTCAGATAAGCTTAAGGGAC
TTAAGTGAGGTCCTTGAAGCAGAAAAAGTTCCTCATAACCAGAGGAAGCCTGAATCAACTCATCTGTTA
GAAAACCAAAATACTCAAGAGATTCCACTTGCCATTAGCAGTTCTTCATTCACGACAAGCAACCACAGT
GTGCAAAACAGAGATCATAGAGGAGGCATGCAGCCCTGTTCTCCTCAGAGATGCCAGCCTCCAGCCAGC
TGCAGCCCTCACGAAAATATACTGCCCTATAAATACACAAGCTGGAGACCACCTTTCCCCAAAAGGTCT
GATAGAAAGGATGTCCAGCACAATGAATGGTACATTGGAGAATACAGCCGCCAGGCAGTGGAAGAGGCA
TTCATGAAGGAGAACAAGGATGGTAGTTTCTTGGTCCGAGATTGTTCCACAAAATCCAAGGAAGAGCCC
TATGTTTTGGCTGTGTTTTATGAGAACAAAGTCTACAATGTAAAAATCCGCTTCCTGGAGAGGAATCAG
CAGTTTGCCCTGGGGACAGGACTCAGAGGAGATGAGAAGTTTGATTCAGTAGAAGACATCATCGAACAC
TACAAGAATTTTCCCATTATACTAATTGATGGGAAAGATAAAACTGGGGTCCACAGGAAACAGTGTCAC
CTCACTCAGCCACTCCCTCTCACCAGACACCTCTTGCCTCTGTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_052964
Insert Size 1287 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052964.2
RefSeq Size 1710 bp
RefSeq ORF 1287 bp
Locus ID 116449
UniProt ID Q7Z7G1
Cytogenetics 4p16.1
MW 49.6 kDa
Summary MIST is a member of the SLP76 family of adaptors (see LCP2, MIM 601603; BLNK, MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling, including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A, MIM 147140)-mediated mast cell degranulation (Cao et al., 1999 PubMed 10562326; Goitsuka et al., 2000, 2001 PubMed 10744659 PubMed 11463797).supplied by OMIM, Mar 2008
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"NM_052964" in other vectors (4)
SKU Description Size Price
RC213867 CLNK (Myc-DDK-tagged)-Human cytokine-dependent hematopoietic cell linker (CLNK) 10 ug
$457.00
RC213867L3 Lenti ORF clone of Human cytokine-dependent hematopoietic cell linker (CLNK), Myc-DDK-tagged 10 ug
$757.00
RC213867L4 Lenti ORF clone of Human cytokine-dependent hematopoietic cell linker (CLNK), mGFP tagged 10 ug
$757.00
RG213867 CLNK (tGFP-tagged) - Human cytokine-dependent hematopoietic cell linker (CLNK) 10 ug
$657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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