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RNF34 (NM_194271) Human Tagged ORF Clone

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SKU
RC211608
RNF34 (Myc-DDK-tagged)-Human ring finger protein 34 (RNF34), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF34
Synonyms CARP-1; CARP1; hRFI; RFI; RIF; RIFF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211608 representing NM_194271
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAAGGCGGGTGCCACGTCTATGTGGGCTTCGTGCTGTGGGCTGCTGAATGAAGTCATGGGAACTG
GAGCTGTCAGGGGCCAGCAGTCAGCATTTGCAGGAGCCACCGGTCCATTCAGATTTACACCAAACCCTGA
GTTTTCCACCTACCCACCAGCAGCTACGGAAGGGCCCAACATAGTTTGTAAAGCCTGTGGGCTTTCATTT
TCAGTCTTTAGAAAGAAGCATGTTTGCTGTGACTGCAAGAAGGATTTTTGCTCCGTTTGTTCAGTCTTAC
AAGAAAATCTCCGTAGATGTTCTACTTGTCACTTATTACAAGAGACAGCATTTCAGCGCCCTCAGTTAAT
GCGACTGAAGGTGAAGGACCTGCGGCAGTATCTCATTCTGAGAAATATACCCATAGATACTTGTCGTGAG
AAAGAAGACTTGGTGGATCTAGTACTGTGCCATCATGGACTAGGCTCTGAGGACGACATGGACACAAGCA
GTCTGAATTCTTCAAGGTCCCAGACTTCTAGCTTTTTTACACGTTCGTTTTTTTCAAACTATACAGCCCC
CTCTGCTACTATGTCTTCGTTTCAGGGAGAGCTTATGGATGGAGACCAAACATCCAGATCTGGAGTGCCG
GCACAGGTACAAAGTGAAATCACTTCAGCAAACACAGAAGATGATGATGACGACGATGATGAGGATGATG
ATGATGAAGAAGAAAACGCAGAGGATCGGAACCCCGGGCTCTCCAAGGAGAGAGTGAGAGCTTCACTGTC
TGACTTGTCAAGCCTTGATGATGTGGAAGGAATGAGCGTGCGCCAGCTGAAGGAAATTCTGGCTCGGAAT
TTTGTCAACTATTCTGGCTGTTGTGAAAAATGGGAACTGGTAGAGAAAGTAAACCGGTTATACAAAGAGA
ATGAAGAAAACCAAAAGTCCTATGGCGAGCGGCTGCAGCTGCAGGATGAGGAAGACGACAGCCTGTGTCG
CATCTGCATGGATGCCGTCATCGACTGTGTCCTACTGGAGTGTGGGCACATGGTTACCTGCACCAAGTGC
GGCAAGCGCATGAGTGAGTGTCCCATCTGCCGGCAGTATGTGGTGCGAGCCGTGCACGTGTTCAAGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211608 representing NM_194271
Red=Cloning site Green=Tags(s)

MRKAGATSMWASCCGLLNEVMGTGAVRGQQSAFAGATGPFRFTPNPEFSTYPPAATEGPNIVCKACGLSF
SVFRKKHVCCDCKKDFCSVCSVLQENLRRCSTCHLLQETAFQRPQLMRLKVKDLRQYLILRNIPIDTCRE
KEDLVDLVLCHHGLGSEDDMDTSSLNSSRSQTSSFFTRSFFSNYTAPSATMSSFQGELMDGDQTSRSGVP
AQVQSEITSANTEDDDDDDDEDDDDEEENAEDRNPGLSKERVRASLSDLSSLDDVEGMSVRQLKEILARN
FVNYSGCCEKWELVEKVNRLYKENEENQKSYGERLQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKC
GKRMSECPICRQYVVRAVHVFKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_194271
ORF Size 1119 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194271.2
RefSeq Size 2105 bp
RefSeq ORF 1122 bp
Locus ID 80196
UniProt ID Q969K3
Cytogenetics 12q24.31
Protein Families Druggable Genome
MW 41.6 kDa
Summary The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Feb 2012
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Documents
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"NM_194271" in other vectors (6)
SKU Description Size Price
RC211608L1 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211608L2 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, mGFP tagged 10 ug
$757.00
RC211608L3 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211608L4 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, mGFP tagged 10 ug
$757.00
RG211608 RNF34 (tGFP-tagged) - Human ring finger protein 34 (RNF34), transcript variant 1 10 ug
$489.00 $657.00
SC107733 RNF34 (untagged)-Human ring finger protein 34 (RNF34), transcript variant 1 10 ug
$457.00

Plasmid Map for RC211608

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211608
Circular map for RC211608

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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