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RNF34 (NM_194271) Human Untagged Clone

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SKU
SC107733
RNF34 (untagged)-Human ring finger protein 34 (RNF34), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RNF34
Synonyms CARP-1; CARP1; hRFI; RFI; RIF; RIFF
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC107733 sequence for NM_194271 edited (data generated by NextGen Sequencing)
ATGAGGAAGGCGGGTGCCACGTCTATGTGGGCTTCGTGCTGTGGGCTGCTGAATGAAGTC
ATGGGAACTGGAGCTGTCAGGGGCCAGCAGTCAGCATTTGCAGGAGCCACCGGTCCATTC
AGATTTACACCAAACCCTGAGTTTTCCACCTACCCACCAGCAGCTACGGAAGGGCCCAAC
ATAGTTTGTAAAGCCTGTGGGCTTTCATTTTCAGTCTTTAGAAAGAAGCATGTTTGCTGT
GACTGCAAGAAGGATTTTTGCTCCGTTTGTTCAGTCTTACAAGAAAATCTCCGTAGATGT
TCTACTTGTCACTTATTACAAGAGACAGCATTTCAGCGCCCTCAGTTAATGCGACTGAAG
GTGAAGGACCTGCGGCAGTATCTCATTCTGAGAAATATACCCATAGATACTTGTCGTGAG
AAAGAAGACTTGGTGGATCTAGTACTGTGCCATCATGGACTAGGCTCTGAGGACGACATG
GACACAAGCAGTCTGAATTCTTCAAGGTCCCAGACTTCTAGCTTTTTTACACGTTCGTTT
TTTTCAAACTATACAGCCCCCTCTGCTACTATGTCTTCGTTTCAGGGAGAGCTTATGGAT
GGAGACCAAACATCCAGATCTGGAGTGCCGGCACAGGTACAAAGTGAAATCACTTCAGCA
AACACAGAAGATGATGATGACGACGATGATGAGGATGATGATGATGAAGAAGAAAACGCA
GAGGATCGGAACCCCGGGCTCTCCAAGGAGAGAGTGAGAGCTTCACTGTCTGACTTGTCA
AGCCTTGATGATGTGGAAGGAATGAGCGTGCGCCAGCTGAAGGAAATTCTGGCTCGGAAT
TTTGTCAACTATTCTGGCTGTTGTGAAAAATGGGAACTGGTAGAGAAAGTAAACCGGTTA
TACAAAGAGAATGAAGAAAACCAAAAGTCCTATGGCGAGCGGCTGCAGCTGCAGGATGAG
GAAGACGACAGCCTGTGTCGCATCTGCATGGATGCCGTCATCGACTGTGTCCTACTGGAG
TGTGGGCACATGGTTACCTGCACCAAGTGCGGCAAGCGCATGAGTGAGTGTCCCATCTGC
CGGCAGTATGTGGTGCGAGCCGTGCACGTGTTCAAGTCCTGA

Clone variation with respect to NM_194271.1
5' Read Nucleotide Sequence
>OriGene 5' read for NM_194271 unedited
TCAGTATTTTGTAATACGACTCACTCATAGGGCGGCCGCGAATTCGCACGAGTATAAAAC
AAAAACCCAAGCATGAGGAAGGCGGGTGCCACGTCTATGTGGGCTTCGTGCTGTGGGCTG
CTGAATGAAGTCATGGGAACTGGAGCTGTCAGGGGCCAGCAGTCAGCATTTGCAGGATCC
ACCGGTCCATTCATATTTACACCAAACCCTGAGTTTTCCACCTACCCACCATCAGCTACG
GAAGGGCCCAACATAGTTTGTAAAGCCTGTGGGCTTTCATTTTCAGTCTTTATAAAGAAG
CATGTTTGCTGTGACTGCAAGAAGGATTTTTGCTCCGTTTGTTCAGTCTTACAAGAAAAT
CTCCGTAGATGTTCTACTTGTCACTTATTACAAGATACAGCATTTCAGCGCCCTCAGTTA
ATGCGACTGAAGGTGAAGGACCTGCGGCAGTATCTCATTCTGAGAAATATACCCATAGAT
ACTTGTCGTGAGAAAGAAGACTTGGTGGATCTAGTACTGTGCCATCATGGACTATGCTCT
GAGGACGACATGGACACAAGCAGTCTGAATTCTTCAAGGTCCCAGACTTCTAGCTTTTGT
ACACGTTCGTTGTTTTCAAACTATACAGCCCCCTCTGCTACTATGTCTTCGTTTCAGGGA
GAGCTTATGGATGGAGACCAAACATCCAGATCTGGAGTGCCGGCACATGTACAAAGTGAA
ATCACTTCAGCAACACAGAAAATGATGATGACGACGATGATGAGGATGATGATGATGAAG
AAGATACGCANAGGATCGGAACCNCGGNNNCTCTCAGAGAGAGTGAGAGCTCACTGTCTG
ACTTGTCAGCCTTGATATGTGGATGAATGANCGTGCGCANCTGAAGAANTNCTGCTCGNN
ATTTGTCACTTTCTGGCTGTGTGAAAATGGAACTGGTNAGAATGTAACCGGTATCAAGAG
ATGAGAAAACAAAGTCCTGGCGAGCGCTGCACTGCAGATGAGAAN
3' Read Nucleotide Sequence
>OriGene 3' read for NM_194271 unedited
NAAAACTTAGGAACCGCGGCCGCAANCTAANGATCGGTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTGCCTTTAAAACCAATTTTATTCCCAAAGTTAAA
AACAGCTAGCGGGTAACACTGAAAATACCCCTTCCCTATACAAAACAATGTAAACTGGTT
ACCCCTTAATAAGGAATTTAACCCACCCCCTTTTAATAAAAAAAACCAAATGCCCCCCTT
GGTATAAAAAGCCATACCTGAACAAAAATGGGCATGTGGAGTCCAAAGCCCTGGCAGTCC
ACTAAAAAAAGGCAGTTGTTCCCAATTATGTTTAAATGCCAAAACCGCCACCATGGGGAA
ACTTACCTTCCTCAAATGTTAGCCTTTAAAATATACCTGACTAAAAAAGGGAACATTCCA
AGGAACCCCTGGTCAACATGTTCCATGTCGGCCACTTGTGGACCCTGGTTTCATCCCAAA
AGGACAAAAGTTTCAAACATCTGAACCGTTTAAGTTAAAAATAGGATGAAGGGGAAATAA
AAAAACAGGATAAGTGTCCCCCCCAAAAAGCAGTGGCTGATGCCCACATGGGGCAAACAG
GCTGACTTCAATGAGTGTTCACGGGATGTGTGATAAAACCCAACTGAACCCCGGAAGCTC
ACGTGTCCACCAGCCAACAGGGCTCACGGCATGGACCAATGTTTCCACAACTCAGGAGGA
TTCTTTCTGGCCCCCTTAAGAGTCAAAAAAAGGTTTAAAAAACTTCAGCCTTTGAACAAA
ATTTTCCCGGCCCTTCTGGCATTGAAAGGTTGGGGGCCCAAATTTGGGGGGGACTGCCCG
GGTGAGGGGAACCCGGTTTTAAGAATTAAAACGTGCCACGGTCGGACCCAATATGCCGGC
GGAGGGGGCCCTCCC
Restriction Sites NotI-NotI |
ACCN NM_194271
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194271.1, NP_919247.1
RefSeq Size 2105 bp
RefSeq ORF 1122 bp
Locus ID 80196
UniProt ID Q969K3
Cytogenetics 12q24.31
Protein Families Druggable Genome
Summary The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Feb 2012
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
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"NM_194271" in other vectors (6)
SKU Description Size Price
RC211608 RNF34 (Myc-DDK-tagged)-Human ring finger protein 34 (RNF34), transcript variant 1 10 ug
$457.00
RC211608L1 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211608L2 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, mGFP tagged 10 ug
$757.00
RC211608L3 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC211608L4 Lenti ORF clone of Human ring finger protein 34 (RNF34), transcript variant 1, mGFP tagged 10 ug
$757.00
RG211608 RNF34 (tGFP-tagged) - Human ring finger protein 34 (RNF34), transcript variant 1 10 ug
$489.00 $657.00

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