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NIT1 (NM_005600) Human Tagged ORF Clone

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SKU
RC211519
NIT1 (Myc-DDK-tagged)-Human nitrilase 1 (NIT1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NIT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC211519 representing NM_005600
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGGCTTCATCACCAGGCCTCCTCACAGATTCCTGTCCCTTCTGTGTCCTGGACTCCGGATACCTC
AACTCTCAGTACTTTGTGCTCAGCCCAGGCCCAGAGCCATGGCTATCTCCTCTTCCTCCTGCGAACTGCC
CCTGGTGGCTGTGTGCCAGGTAACATCGACGCCAGACAAGCAACAGAACTTTAAAACATGTGCTGAGCTG
GTTCGAGAGGCTGCCAGACTGGGTGCCTGCCTGGCTTTCCTGCCTGAGGCATTTGACTTCATTGCACGGG
ACCCTGCAGAGACGCTACACCTGTCTGAACCACTGGGTGGGAAACTTTTGGAAGAATACACCCAGCTTGC
CAGGGAATGTGGACTCTGGCTGTCCTTGGGTGGTTTCCATGAGCGTGGCCAAGACTGGGAGCAGACTCAG
AAAATCTACAATTGTCACGTGCTGCTGAACAGCAAAGGGGCAGTAGTGGCCACTTACAGGAAGACACATC
TGTGTGACGTAGAGATTCCAGGGCAGGGGCCTATGTGTGAAAGCAACTCTACCATGCCTGGGCCCAGTCT
TGAGTCACCTGTCAGCACACCAGCAGGCAAGATTGGTCTAGCTGTCTGCTATGACATGCGGTTCCCTGAA
CTCTCTCTGGCATTGGCTCAAGCTGGAGCAGAGATACTTACCTATCCTTCAGCTTTTGGATCCATTACAG
GCCCAGCCCACTGGGAGGTGTTGCTGCGGGCCCGTGCTATCGAAACCCAGTGCTATGTAGTGGCAGCAGC
ACAGTGTGGACGCCACCATGAGAAGAGAGCAAGTTATGGCCACAGCATGGTGGTAGACCCCTGGGGAACA
GTGGTGGCCCGCTGCTCTGAGGGGCCAGGCCTCTGCCTTGCCCGAATAGACCTCAACTATCTGCGACAGT
TGCGCCGACACCTGCCTGTGTTCCAGCACCGCAGGCCTGACCTCTATGGCAATCTGGGTCACCCACTGTC
T

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211519 representing NM_005600
Red=Cloning site Green=Tags(s)

MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAEL
VREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQ
KIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPE
LSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGT
VVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005600
ORF Size 981 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005600.3
RefSeq Size 1385 bp
RefSeq ORF 984 bp
Locus ID 4817
UniProt ID Q86X76
Cytogenetics 1q23.3
Domains CN_hydrolase
MW 35.7 kDa
Summary This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2010
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"NM_005600" in other vectors (6)
SKU Description Size Price
RC211519L1 Lenti ORF clone of Human nitrilase 1 (NIT1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC211519L2 Lenti ORF clone of Human nitrilase 1 (NIT1), transcript variant 1, mGFP tagged 10 ug
$600.00
RC211519L3 Lenti ORF clone of Human nitrilase 1 (NIT1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC211519L4 Lenti ORF clone of Human nitrilase 1 (NIT1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG211519 NIT1 (tGFP-tagged) - Human nitrilase 1 (NIT1), transcript variant 1 10 ug
$489.00 $500.00
SC116684 NIT1 (untagged)-Human nitrilase 1 (NIT1), transcript variant 1 10 ug
$300.00

Plasmid Map for RC211519

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC211519
Circular map for RC211519

file-alt Citations

Nit1 is upregulated in non-small cell lung cancer and promotes cancer cell proliferation and invasion and regulates EMT-related molecules and cyclins
Wang, Jiang, Wang et al
J Cancer (2025) 16 (10), 3040-3047 DOI: 10.7150/jca.96095
AI Summary: OriGene’s Nit1 cDNA clone (RC211519) was used to overexpress Nit1 in lung cancer cells, enabling the study to investigate Nit1’s role in promoting proliferation, invasion, and regulation of EMT-related molecules and cyclins in non-small cell lung cancer.
Snippet: ll culture and transfection The cell lines used in this study included HBE, LK2, H1299, A549, PC9, H460, H661 and SK. Cells were cultured according to the guideline of the ATCC/CTCC. Nit1 cDNA clone (Origene, RC211519, Rockville, MD, USA) was transfected using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. Immunofluorescence The detailed protocol w
FigureFigureFigure
Showing 1-1 of 1 papers.
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