Human NIT1 (NM_005600) AAV Particle

SKU RC211519A1V

AAV ORF Particles, serotype AAV-2, NIT1 (Myc-DDK-tagged)-Human nitrilase 1 (NIT1), transcript variant 1, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: NIT1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211519 representing NM_005600; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTGGGCTTCATCACCAGGCCTCCTCACAGATTCCTGTCCCTTCTGTGTCCTGGACTCCGGATACCTC; AACTCTCAGTACTTTGTGCTCAGCCCAGGCCCAGAGCCATGGCTATCTCCTCTTCCTCCTGCGAACTGCC; CCTGGTGGCTGTGTGCCAGGTAACATCGACGCCAGACAAGCAACAGAACTTTAAAACATGTGCTGAGCTG; GTTCGAGAGGCTGCCAGACTGGGTGCCTGCCTGGCTTTCCTGCCTGAGGCATTTGACTTCATTGCACGGG; ACCCTGCAGAGACGCTACACCTGTCTGAACCACTGGGTGGGAAACTTTTGGAAGAATACACCCAGCTTGC; CAGGGAATGTGGACTCTGGCTGTCCTTGGGTGGTTTCCATGAGCGTGGCCAAGACTGGGAGCAGACTCAG; AAAATCTACAATTGTCACGTGCTGCTGAACAGCAAAGGGGCAGTAGTGGCCACTTACAGGAAGACACATC; TGTGTGACGTAGAGATTCCAGGGCAGGGGCCTATGTGTGAAAGCAACTCTACCATGCCTGGGCCCAGTCT; TGAGTCACCTGTCAGCACACCAGCAGGCAAGATTGGTCTAGCTGTCTGCTATGACATGCGGTTCCCTGAA; CTCTCTCTGGCATTGGCTCAAGCTGGAGCAGAGATACTTACCTATCCTTCAGCTTTTGGATCCATTACAG; GCCCAGCCCACTGGGAGGTGTTGCTGCGGGCCCGTGCTATCGAAACCCAGTGCTATGTAGTGGCAGCAGC; ACAGTGTGGACGCCACCATGAGAAGAGAGCAAGTTATGGCCACAGCATGGTGGTAGACCCCTGGGGAACA; GTGGTGGCCCGCTGCTCTGAGGGGCCAGGCCTCTGCCTTGCCCGAATAGACCTCAACTATCTGCGACAGT; TGCGCCGACACCTGCCTGTGTTCCAGCACCGCAGGCCTGACCTCTATGGCAATCTGGGTCACCCACTGTC; T; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211519 representing NM_005600; Red=Cloning site Green=Tags(s); ; MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAEL; VREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQ; KIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPE; LSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGT; VVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_005600
• ORF Size: 981 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_005600.1
• RefSeq Size: 1385 bp
• RefSeq ORF: 984 bp
• Locus ID: 4817
• UniProt ID: Q86X76
• Cytogenetics: 1q23.3
• MW: 35.7 kDa