NIT1 (NM_005600) Human Untagged Clone

SKU SC116684

NIT1 (untagged)-Human nitrilase 1 (NIT1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NIT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116684 sequence for NM_005600 edited (data generated by NextGen Sequencing); ATGCTGGGCTTCATCACCAGGCCTCCTCACAGATTCCTGTCCCTTCTGTGTCCTGGACTC; CGGATACCTCAACTCTCAGTACTTTGTGCTCAGCCCAGGCCCAGAGCCATGGCTATCTCC; TCTTCCTCCTGCGAACTGCCCCTGGTGGCTGTGTGCCAGGTAACATCGACGCCAGACAAG; CAACAGAACTTTAAAACATGTGCTGAGCTGGTTCGAGAGGCTGCCAGACTGGGTGCCTGC; CTGGCTTTCCTGCCTGAGGCATTTGACTTCATTGCACGGGACCCTGCAGAGACGCTACAC; CTGTCTGAACCACTGGGTGGGAAACTTTTGGAAGAATACACCCAGCTTGCCAGGGAATGT; GGACTCTGGCTGTCCTTGGGTGGTTTCCATGAGCGTGGCCAAGACTGGGAGCAGACTCAG; AAAATCTACAATTGTCACGTGCTGCTGAACAGCAAAGGGGCAGTAGTGGCCACTTACAGG; AAGACACATCTGTGTGACGTAGAGATTCCAGGGCAGGGGCCTATGTGTGAAAGCAACTCT; ACCATGCCTGGGCCCAGTCTTGAGTCACCTGTCAGCACACCAGCAGGCAAGATTGGTCTA; GCTGTCTGCTATGACATGCGGTTCCCTGAACTCTCTCTGGCATTGGCTCAAGCTGGAGCA; GAGATACTTACCTATCCTTCAGCTTTTGGATCCATTACAGGCCCAGCCCACTGGGAGGTG; TTGCTGCGGGCCCGTGCTATCGAAACCCAGTGCTATGTAGTGGCAGCAGCACAGTGTGGA; CGCCACCATGAGAAGAGAGCAAGTTATGGCCACAGCATGGTGGTAGACCCCTGGGGAACA; GTGGTGGCCCGCTGCTCTGAGGGGCCAGGCCTCTGCCTTGCCCGAATAGACCTCAACTAT; CTGCGACAGTTGCGCCGACACCTGCCTGTGTTCCAGCACCGCAGGCCTGACCTCTATGGC; AATCTGGGTCACCCACTGTCTTAA; ; Clone variation with respect to NM_005600.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005600 unedited; GTAATACGACCTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTCCATCTTCCCACTGT; ATGGTCTTGTCCCTTAGCCTATAATTTCTTATAAAGAGCGTTTTGAAGACTAGGTTTTTA; CTGGCAGGGCCCTGATTCAGGCTCACAGTATAAAGAGAGAAGTCAGAGAATCCTAGAAAT; TTATTGCTTGAAGAAGGGGTTGGTGTCCTCATATCTCACCTTCCTCAGGCTGGGCTTCAT; CACCAGGCCTCCTCACAGATTCCTGTCCCTTCTGTGTCCTGGACTCCGGATACCTCAACT; CTCAGTACTTTGTGCTCAGCCCAGGCCCAGAGCCATGGCTATCTCCTCTTCCTCCTGCGA; ACTGCCCCTGGTGGCTGTGTGCCAGGTAACATCGACGCCAGACAAGCAACAGAACTTTAA; AACATGTGCTGAGCTGGTTCGAGAGGCTGCCAGACTGGGTGCCTGCCTGGCTTTCCTGCC; TGAGGCATTTGACTTCATTGCACGGGACCCTGCAGAGACGCTACACCTGTCTGAACCACT; GGGTGGGAAACTTTTGGAAGAATACACCCAGCTTGCCAGGGAATGTGGACTCTGGCTGTC; CTTGGGTGGTTTCCATGAGCGTGGCCAAGACTGGGAGCAGACTCAGAAAATCTACAATTG; TCACGTGCTGCTGAACAGCAAAGGGGCAGTAGTGGCCACTTACAGGAAGACACATCTGTG; TGACGTAGAGATTCCAGGGCAGGNNGCCTATGTGTGAAAGCAACTCTACCATGCCTGGGC; CCAGTCTTGAGTCACCTGTCAGCACACCAGCAGGCAAGATTGGTCTAGCTGTCTGCTATG; ACATGCNGNTCCCCTGACTCTCTCTGGCATTGGCTCAAGCTGGACAGAGATACTTACCTA; TCCTTCACTTTTNGGATCCATACAGGNCCCAGCACTGGGGGAGTGTTGCTGGGGGCCCGT; GCTATCNAACCCANTGCTTTGTATGCAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005600 unedited; CGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTGATTATTATATTTTTCAATGC; CAGTGCTGCTCAGCCCTCAGCAGAACTTCAGTTTCCATGAAATAAACAATGACTATATAA; AATTCCACCTTTCTGAAACTGCAGTCTGACCTCAGGTGAAGCTCAAGTGAAAGTTTCTTT; CCCAAGCAGCCCATCTGTGTTCCATCAAGAGAGTCAAGGTTCTCCAAGTGAGGGGAGCTG; TGCCTGGATCCTGCCTCCAAGTCACATGAGCACTAGCTCATATTGGCAGGGTGGGGGTGG; GAGGGGCAGGTCTAAACTCACAGAAGTCAAGTCTTAAGACAGTGGGTGACCCAGATTGCC; ATAGAGGTCAAGCCTGCGGTGCTGGAACACAGGCAGGTGTCGGCGCAACTGTCGCAGATA; GTTGAGGTCTATTCGGGCAAAGCAGAGGCCTGGCCCCTCAGAGCAGCGGGCCACCACTGT; TCCCCAGGGGTCTACCACCATGCTGAGGCCATAACTTGCTCTCTTTTATGGGGGCGTCCA; CACTGTGCTGCTGCACTACTATCACTGGGTTTCGATACACGGGCCCGAGCAACACCTTCC; AGGGGCTGGGCCTGAATGGATCCAAAGCCTGAGGATAGGTAGTATCTCTGCTCCATTTGA; GCCATGCCAAAAAGAGTTCAGGAACCGCTTGCCTTTCAGACAGTTAACAATCTTGCCTGC; TGTGTGCTCGCAGGGGACTAAAAATGGCCCATGCATGGACAATTGCTTTACACATAGCCC; CCGCCCCGGAACCCTACCCAAAACATTGTTTTCCTTTATGGCCCCCCGCCCCTTTCTGCC; ACACCCCCCCAATGGTTTTTCGCCCTTCCCCCCCTGGCCCTCTCTGATTCACCAGGACTC; TCTCCCTCTTCCTGGAACGGGTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005600
• Insert Size: 1580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005600.1, NP_005591.1
• RefSeq Size: 1385 bp
• RefSeq ORF: 984 bp
• Locus ID: 4817
• UniProt ID: Q86X76
• Cytogenetics: 1q23.3
• Domains: CN_hydrolase
• Summary: This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]; Transcript Variant: This variant (1) represents the shortest transcript and encodes the longest isoform (1).