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PITX2 (NM_153427) Human Tagged ORF Clone

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SKU
RC210612
PITX2 (Myc-DDK-tagged)-Human paired-like homeodomain 2 (PITX2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PITX2
Synonyms ARP1; ASGD4; Brx1; IDG2; IGDS; IGDS2; IHG2; IRID2; Otlx2; PTX2; RGS; RIEG; RIEG1; RS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210612 representing NM_153427
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACCAACTGCCGCAAACTGGTGTCGGCGTGTGTGCAATTAGAGAAAGATAAAAGCCAGCAGGGGA
AGAATGAGGACGTGGGCGCCGAGGACCCGTCTAAGAAGAAGCGGCAAAGGCGGCAGCGGACTCACTTTAC
CAGCCAGCAGCTCCAGGAGCTGGAGGCCACTTTCCAGAGGAACCGCTACCCGGACATGTCCACACGCGAA
GAAATCGCTGTGTGGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGAATCGTCGGGCCAAAT
GGAGAAAGAGGGAGCGCAACCAGCAGGCCGAGCTATGCAAGAATGGCTTCGGGCCGCAGTTCAATGGGCT
CATGCAGCCCTACGACGACATGTACCCAGGCTATTCCTACAACAACTGGGCCGCCAAGGGCCTTACATCC
GCCTCCCTATCCACCAAGAGCTTCCCCTTCTTCAACTCTATGAACGTCAACCCCCTGTCATCACAGAGCA
TGTTTTCCCCACCCAACTCTATCTCGTCCATGAGCATGTCGTCCAGCATGGTGCCCTCAGCAGTGACAGG
CGTCCCGGGCTCCAGTCTCAACAGCCTGAATAACTTGAACAACCTGAGTAGCCCGTCGCTGAATTCCGCG
GTGCCGACGCCTGCCTGTCCTTACGCGCCGCCGACTCCTCCGTATGTTTATAGGGACACGTGTAACTCGA
GCCTGGCCAGCCTGAGACTGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAGCGTGCAGAACCCGGC
CTCCAACCTGAGTGCTTGCCAGTATGCAGTGGACCGGCCCGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210612 representing NM_153427
Red=Cloning site Green=Tags(s)

METNCRKLVSACVQLEKDKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTRE
EIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTS
ASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSA
VPTPACPYAPPTPPYVYRDTCNSSLASLRLKAKQHSSFGYASVQNPASNLSACQYAVDRPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153427
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153427.2, NP_700476.1
RefSeq Size 2122 bp
RefSeq ORF 816 bp
Locus ID 5308
UniProt ID Q99697
Cytogenetics 4q25
Protein Families Transcription Factors
Protein Pathways TGF-beta signaling pathway
MW 30.2 kDa
Summary This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. The encoded protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. This protein plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this gene are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development. Alternatively spliced transcript variants encoding distinct isoforms have been described. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_153427" in other vectors (6)
SKU Description Size Price
RC210612L1 Lenti ORF clone of Human paired-like homeodomain 2 (PITX2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC210612L2 Lenti ORF clone of Human paired-like homeodomain 2 (PITX2), transcript variant 1, mGFP tagged 10 ug
$600.00
RC210612L3 Lenti ORF clone of Human paired-like homeodomain 2 (PITX2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC210612L4 Lenti ORF clone of Human paired-like homeodomain 2 (PITX2), transcript variant 1, mGFP tagged 10 ug
$600.00
RG210612 PITX2 (tGFP-tagged) - Human paired-like homeodomain 2 (PITX2), transcript variant 1 10 ug
$489.00 $500.00
SC306602 PITX2 (untagged)-Human paired-like homeodomain 2 (PITX2), transcript variant 1 10 ug
$330.00

Plasmid Map for RC210612

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210612
Circular map for RC210612

file-alt Citations

PITX2 and non-canonical Wnt pathway interaction in metastatic prostate cancer
Vela, Morrissey, Zhang et al
Clin. Exp. Metastasis (2013)
Cited by 25
AI Summary: OriGene contributed MYC- and FLAG-tagged pCMV6 entry vector human PITX2 cDNA clones (TrueORF) for overexpression of individual PITX2A/B/C isoforms, enabling the study of their roles in prostate cancer cell motility and metastasis.
Snippet: Plasmids for over expression of individual Pitx2 iso- forms (human cDNA clones of transcript variants 1 (NM_153427), 2 (NM_153426) and 3 (NM_000325)) in a MYC- and FLAG-tagged pCMV6 entry vector were pur- chased from Origene (TrueORF, Origene
Showing 1-1 of 1 papers.
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