BCL3 (NM_005178) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BCL3 |
| Synonyms | BCL4; D19S37 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005178 |
| ORF Size | 1338 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005178.3, NP_005169.1 |
| RefSeq Size | 1864 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 602 |
| UniProt ID | P20749 |
| Cytogenetics | 19q13.32 |
| Domains | ANK |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 47.4 kDa |
| Summary | This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005178" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209116L1 | Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC209116L2 | Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), mGFP tagged | 10 ug |
$757.00
|
|
| RC209116L3 | Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC209116L4 | Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), mGFP tagged | 10 ug |
$757.00
|
|
| RG209116 | BCL3 (tGFP-tagged) - Human B-cell CLL/lymphoma 3 (BCL3) | 10 ug |
$489.00
$657.00
|
|
| SC128102 | BCL3 (untagged)-Human B-cell CLL/lymphoma 3 (BCL3) | 10 ug |
$457.00
|
|
| SC317743 | BCL3 (untagged)-Human B-cell CLL/lymphoma 3 (BCL3) | 10 ug |
$503.00
|
|
Citations
Bcl3: a regulator of NF-κB inducible by TWEAK in acute kidney injury with anti-inflammatory and antiapoptotic properties in tubular cells
Poveda, Sanz, Carrasco et al
Exp. Mol. Med. (2017) 49 (7), e352 DOI: 10.1038/emm.2017.89
Poveda, Sanz, Carrasco et al
Exp. Mol. Med. (2017) 49 (7), e352 DOI: 10.1038/emm.2017.89
AI Summary: OriGene's Bcl3 overexpressing plasmid (pCMV6 vector) was used to transfect tubular cells, enabling the study of Bcl3's role in regulating inflammation and cell death during acute kidney injury.
Snippet: Cells were grown in six-well plates (Costar) and transfected with a mixture of 0.5 μ g Bcl3 overexpressing plasmid in the pCMV6 vector (OriGene, Rockville, MD, USA), Opti-MEM I Reduced Serum Medium and Lipofectamine 3000 transfection reagent (Invitrogen) following the manufacturer ’ s instructions
Bcl-3, induced by Tax and HTLV-1, inhibits NF-κB activation and promotes autophagy
Wang, Niu, Shi et al
Cell. Signal. (2013)
Wang, Niu, Shi et al
Cell. Signal. (2013)
Cited by 16
AI Summary: OriGene’s pCMV-entry-Bcl-3 plasmid (RC209116) was used as the source of Bcl-3 cDNA to construct expression vectors for studying Bcl-3’s role in NF-κB regulation and autophagy in HTLV-1-infected cells.
Snippet: To construct a plasmid expressing full-length Bcl-3 without a tag, the cDNA of Bcl-3 was subcloned from pCMV-entry-Bcl-3 (Origene, RC209116) into the EcoR I and Xho I sites of the pcDNA3.0 vector using the following primers: 5'-cgggaattcGTCGACTGGATCCGGTACC-3'and 5
Showing 1-2 of 2 papers.
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