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BCL3 (NM_005178) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG209116
BCL3 (tGFP-tagged) - Human B-cell CLL/lymphoma 3 (BCL3)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BCL3
Synonyms BCL4; D19S37
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG209116 representing NM_005178
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGAGGGGCCCGTGGACCTGCGCACCCGGCCCAAGGCCGCCGGACTCCCGGGCGCCGCGCTGCCGC
TCCGCAAGCGCCCGCTGCGCGCGCCCTCCCCGGAGCCCGCCGCTCCCCGCGGCGCTGCGGGCCTTGTCGT
CCCCCTGGACCCTCTGCGCGGCGGCTGCGACCTGCCGGCGGTCCCCGGGCCCCCCCACGGCCTGGCCCGG
CCGGAGGCGCTTTACTACCCCGGAGCCTTACTGCCTTTGTACCCCACTCGGGCCATGGGCTCCCCGTTTC
CTCTGGTGAACCTGCCTACACCCCTATACCCCATGATGTGCCCCATGGAACACCCCCTTTCTGCTGACAT
CGCCATGGCCACCCGTGCAGATGAGGACGGAGACACGCCTCTCCATATTGCTGTGGTGCAGGGTAACCTG
CCAGCTGTGCACCGGCTGGTCAACCTCTTCCAGCAGGGGGGCCGGGAGCTCGACATCTACAACAACCTAC
GGCAGACACCGCTCCACCTGGCTGTGATCACCACATTACCGTCTGTGGTCCGGCTCCTGGTGACAGCTGG
TGCCAGCCCCATGGCGCTGGACCGCCATGGCCAGACGGCCGCTCACCTGGCGTGCGAGCACCGCAGCCCG
ACCTGCCTGCGAGCCCTGCTGGACAGCGCAGCTCCGGGCACGTTGGACCTGGAGGCCCGCAATTATGACG
GGCTCACCGCCCTGCACGTGGCAGTGAACACCGAGTGCCAAGAAACCGTGCAGCTCTTGCTAGAGCGCGG
TGCCGACATCGACGCAGTGGACATTAAGAGCGGCCGCTCCCCGCTCATCCACGCCGTGGAAAACAACAGC
CTTAGCATGGTGCAGCTGCTGCTGCAGCACGGCGCCAACGTGAACGCGCAAATGTACTCCGGCAGCTCCG
CCCTGCACTCAGCGTCCGGCCGCGGGCTCCTCCCGCTGGTGCGCACGCTGGTCCGCAGCGGCGCTGACAG
CAGCCTCAAGAACTGCCACAACGACACGCCGCTCATGGTGGCGCGCAGCCGCAGGGTCATCGACATCCTG
AGGGGGAAGGCCACCCGGCCTGCTTCCACCTCCCAGCCAGACCCCTCCCCTGACCGGAGCGCCAACACCT
CCCCCGAGAGCAGCAGCCGCCTCAGCTCCAATGGTCTTCTCTCCGCATCACCATCCTCCTCACCCTCCCA
GTCTCCCCCCAGGGACCCCCCTGGATTCCCCATGGCTCCTCCCAATTTCTTCCTTCCTTCCCCATCTCCA
CCCGCCTTCCTGCCCTTTGCTGGGGTCCTCCGAGGCCCTGGCCGGCCGGTGCCCCCCTCCCCAGCTCCAG
GAGGCAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG209116 representing NM_005178
Red=Cloning site Green=Tags(s)

MDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLAR
PEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNL
PAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSP
TCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNS
LSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDIL
RGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSP
PAFLPFAGVLRGPGRPVPPSPAPGGS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005178
ORF Size 1338 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005178.3, NP_005169.1
RefSeq Size 1879 bp
RefSeq ORF 1365 bp
Locus ID 602
UniProt ID P20749
Cytogenetics 19q13.32
Domains ANK
Protein Families Druggable Genome, Transcription Factors
Summary This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. provided by RefSeq, Jul 2008
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"NM_005178" in other vectors (7)
SKU Description Size Price
RC209116 BCL3 (Myc-DDK-tagged)-Human B-cell CLL/lymphoma 3 (BCL3) 10 ug
$457.00
RC209116L1 Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), Myc-DDK-tagged 10 ug
$757.00
RC209116L2 Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), mGFP tagged 10 ug
$757.00
RC209116L3 Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), Myc-DDK-tagged 10 ug
$757.00
RC209116L4 Lenti ORF clone of Human B-cell CLL/lymphoma 3 (BCL3), mGFP tagged 10 ug
$757.00
SC128102 BCL3 (untagged)-Human B-cell CLL/lymphoma 3 (BCL3) 10 ug
$457.00
SC317743 BCL3 (untagged)-Human B-cell CLL/lymphoma 3 (BCL3) 10 ug
$503.00

Plasmid Map for RG209116

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG209116
Circular map for RG209116

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