OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

TPX2 (NM_012112) Human Tagged Lenti ORF Clone

  • LentiORF®
Be the first to review this product
SKU
RC205821L1
Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $984.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00
GE100111 Forward primer to sequence targets cloned in expression vectors.
1 nmol
$71.00
GE100122 Reverse primer to sequence targets cloned in expression vectors.
1 nmol
$71.00
TR30037 Lenti-vpak packaging kit - packaging plasmids and transfection reagent
10 reactions
$720.00

Other products for "TPX2 or NM_012112"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TPX2
Synonyms C20orf1; C20orf2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; p100; REPP86
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC205821).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012112
ORF Size 2241 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012112.4
RefSeq Size 3685 bp
RefSeq ORF 2244 bp
Locus ID 22974
UniProt ID Q9ULW0
Cytogenetics 20q11.21
Protein Families Druggable Genome, Stem cell - Pluripotency
MW 85.7 kDa
Summary Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_012112" in other vectors (6)
SKU Description Size Price
RC205821 TPX2 (Myc-DDK-tagged)-Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2) 10 ug
$684.00
RC205821L2 Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), mGFP tagged 10 ug
$984.00
RC205821L3 Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), Myc-DDK-tagged 10 ug
$984.00
RC205821L4 Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), mGFP tagged 10 ug
$984.00
RG205821 TPX2 (tGFP-tagged) - Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2) 10 ug
$789.00 $884.00
SC115515 TPX2 (untagged)-Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2) 10 ug
$685.00

Plasmid Map for RC205821L1

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205821L1
Circular map for RC205821L1

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products