TPX2 (NM_012112) Human Tagged ORF Clone
SKU
RC205821
TPX2 (Myc-DDK-tagged)-Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2)
$684.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TPX2 |
| Synonyms | C20orf1; C20orf2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; p100; REPP86 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_012112 |
| ORF Size | 2241 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_012112.5 |
| RefSeq Size | 3685 bp |
| RefSeq ORF | 2244 bp |
| Locus ID | 22974 |
| UniProt ID | Q9ULW0 |
| Cytogenetics | 20q11.21 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| MW | 85.7 kDa |
| Summary | Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_012112" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205821L1 | Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), Myc-DDK-tagged | 10 ug |
$984.00
|
|
| RC205821L2 | Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), mGFP tagged | 10 ug |
$984.00
|
|
| RC205821L3 | Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), Myc-DDK-tagged | 10 ug |
$984.00
|
|
| RC205821L4 | Lenti ORF clone of Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2), mGFP tagged | 10 ug |
$984.00
|
|
| RG205821 | TPX2 (tGFP-tagged) - Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2) | 10 ug |
$789.00
$884.00
|
|
| SC115515 | TPX2 (untagged)-Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2) | 10 ug |
$685.00
|
|
Citations
The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles
Ma, Matsunaga, Morimoto et al
J Cell Sci (2011) 124 (Pt 3), 394-404 DOI: 10.1242/jcs.063347
Ma, Matsunaga, Morimoto et al
J Cell Sci (2011) 124 (Pt 3), 394-404 DOI: 10.1242/jcs.063347
Cited by 32
AI Summary: OriGene products, specifically the SAF-A (hnRNP-U) shRNA (SR304062) and SAF-A (hnRNP-U) antibody (TA500494), were used in this study to deplete SAF-A expression and detect SAF-A protein, enabling the authors to investigate its role in kinetochore-microtubule attachment and mitotic spindle organization.
Showing 1-1 of 1 papers.
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