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SV2B (NM_014848) Human Tagged ORF Clone

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SKU
RC205345
SV2B (Myc-DDK-tagged)-Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $625.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SV2B
Synonyms HsT19680
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC205345 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGACTACAAGTATCAGGACAATTATGGGGGCTATGCTCCCAGTGATGGCTATTACCGCGGCAATG
AGTCCAACCCAGAAGAAGATGCACAGAGTGATGTCACCGAAGGCCATGATGAGGAAGACGAGATCTATGA
GGGCGAGTACCAGGGTATCCCTCACCCAGATGATGTCAAGGCCAAGCAGGCCAAGATGGCGCCCTCCAGA
ATGGACAGCCTTCGGGGCCAGACAGACCTGATGGCTGAGAGGCTGGAAGATGAGGAGCAGTTGGCCCATC
AGTACGAGACCATCATGGATGAGTGTGGCCATGGCCGCTTCCAGTGGATCCTCTTTTTCGTCTTGGGTTT
GGCCCTGATGGCCGATGGGGTGGAAGTGTTCGTGGTGAGTTTTGCCCTGCCCAGTGCAGAGAAGGACATG
TGTCTGTCCAGTTCCAAAAAAGGAATGCTAGGGATGATAGTCTACTTGGGAATGATGGCGGGCGCCTTCA
TCCTGGGAGGCCTGGCTGATAAGCTGGGAAGGAAGCGAGTCCTCAGCATGTCTCTGGCCGTCAATGCCTC
CTTCGCCTCCCTCTCTTCCTTCGTGCAGGGATATGGAGCCTTCCTCTTCTGCCGACTCATCTCAGGCATC
GGTATTGGGGGTGCTCTACCGATTGTTTTTGCCTATTTTTCTGAATTCTTGTCTCGGGAGAAGCGAGGAG
AACACCTCAGTTGGCTGGGCATCTTCTGGATGACTGGGGGCCTGTACGCATCTGCCATGGCCTGGAGCAT
CATCCCACACTATGGCTGGGGCTTCAGCATGGGGACCAATTACCACTTCCATAGCTGGAGAGTGTTTGTC
ATCGTCTGTGCTCTGCCCTGCACCGTGTCCATGGTGGCCCTGAAGTTCATGCCAGAGAGCCCAAGGTTTC
TGCTAGAGATGGGCAAACATGATGAAGCCTGGATGATTCTCAAGCAAGTCCATGACACCAACATGAGAGC
TAAGGGGACCCCAGAGAAAGTGTTCACGGTTTCCAACATCAAAACTCCCAAGCAAATGGATGAATTCATT
GAGATCCAAAGTTCAACAGGAACCTGGTACCAGCGCTGGCTGGTCAGATTCAAGACCATTTTCAAGCAGG
TCTGGGATAATGCCCTGTACTGTGTGATGGGGCCCTACAGAATGAATACACTGATTCTGGCCGTGGTTTG
GTTTGCCATGGCATTCAGTTACTATGGACTGACAGTTTGGTTTCCTGATATGATCCGCTATTTTCAAGAT
GAAGAATACAAGTCTAAAATGAAGGTGTTTTTTGGTGAGCATGTGTACGGCGCCACAATCAACTTCACGA
TGGAAAATCAGATCCACCAACATGGGAAACTTGTGAATGATAAGTTCACAAGAATGTACTTTAAACATGT
ACTCTTTGAGGACACATTCTTTGACGAGTGCTATTTTGAAGACGTAACATCAACAGATACCTACTTCAAA
AATTGTACCATTGAATCAACCATCTTTTACAACACAGACCTCTACGAGCACAAGTTCATCAACTGTCGGT
TTATCAACTCCACCTTCCTGGAGCAGAAGGAGGGCTGCCACATGGACTTGGAGCAAGATAATGACTTCCT
GATTTACCTCGTCAGCTTCCTGGGCAGCCTGTCTGTCTTACCCGGGAACATCATTTCTGCCCTGCTCATG
GATAGAATTGGAAGGCTCAAGATGATTGGTGGCTCCATGCTAATCTCTGCAGTCTGCTGCTTCTTCCTGT
TTTTTGGCAACAGTGAGTCTGCAATGATCGGCTGGCAGTGCCTGTTCTGTGGGACAAGCATTGCAGCCTG
GAATGCTCTGGATGTGATCACAGTGGAGCTGTATCCCACCAACCAGAGAGCAACAGCCTTCGGCATTCTC
AATGGATTATGCAAATTTGGCGCCATCCTGGGAAACACCATCTTTGCTTCTTTTGTTGGGATAACCAAAG
TGGTCCCCATCCTTCTGGCTGCTGCTTCTCTGGTTGGGGGTGGCCTGATTGCCCTTCGACTGCCAGAGAC
TCGAGAACAGGTCCTGATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC205345 protein sequence
Red=Cloning site Green=Tags(s)

MDDYKYQDNYGGYAPSDGYYRGNESNPEEDAQSDVTEGHDEEDEIYEGEYQGIPHPDDVKAKQAKMAPSR
MDSLRGQTDLMAERLEDEEQLAHQYETIMDECGHGRFQWILFFVLGLALMADGVEVFVVSFALPSAEKDM
CLSSSKKGMLGMIVYLGMMAGAFILGGLADKLGRKRVLSMSLAVNASFASLSSFVQGYGAFLFCRLISGI
GIGGALPIVFAYFSEFLSREKRGEHLSWLGIFWMTGGLYASAMAWSIIPHYGWGFSMGTNYHFHSWRVFV
IVCALPCTVSMVALKFMPESPRFLLEMGKHDEAWMILKQVHDTNMRAKGTPEKVFTVSNIKTPKQMDEFI
EIQSSTGTWYQRWLVRFKTIFKQVWDNALYCVMGPYRMNTLILAVVWFAMAFSYYGLTVWFPDMIRYFQD
EEYKSKMKVFFGEHVYGATINFTMENQIHQHGKLVNDKFTRMYFKHVLFEDTFFDECYFEDVTSTDTYFK
NCTIESTIFYNTDLYEHKFINCRFINSTFLEQKEGCHMDLEQDNDFLIYLVSFLGSLSVLPGNIISALLM
DRIGRLKMIGGSMLISAVCCFFLFFGNSESAMIGWQCLFCGTSIAAWNALDVITVELYPTNQRATAFGIL
NGLCKFGAILGNTIFASFVGITKVVPILLAAASLVGGGLIALRLPETREQVLM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014848
ORF Size 2049 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014848.2
RefSeq Size 11361 bp
RefSeq ORF 2052 bp
Locus ID 9899
UniProt ID Q7L1I2
Cytogenetics 15q26.1
Domains sugar_tr
Protein Families Secreted Protein, Transmembrane
Protein Pathways ECM-receptor interaction
MW 77.4 kDa
Summary This gene encodes a member of the synaptic vesicle proteins 2 (SV2) family and major facilitator superfamily of proteins. This protein and other members of the family are localized to synaptic vesicles and may function in the regulation of vesicle trafficking and exocytosis. Studies in mice suggest that the encoded protein may act as a protein receptor for botulinum neurotoxin E in neurons, and that this protein may be important for the integrity of the glomerular filtration barrier. This gene shows reduced expression in areas of synaptic loss in the hippocampus of human temporal lobe epilepsy patients. provided by RefSeq, Sep 2016
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"NM_014848" in other vectors (6)
SKU Description Size Price
RC205345L1 Lenti ORF clone of Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1, Myc-DDK-tagged 10 ug
$925.00
RC205345L2 Lenti ORF clone of Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1, mGFP tagged 10 ug
$925.00
RC205345L3 Lenti ORF clone of Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1, Myc-DDK-tagged 10 ug
$925.00
RC205345L4 Lenti ORF clone of Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1, mGFP tagged 10 ug
$925.00
RG205345 SV2B (tGFP-tagged) - Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1 10 ug
$789.00 $825.00
SC108749 SV2B (untagged)-Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1 10 ug
$3,011.00

Plasmid Map for RC205345

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC205345
Circular map for RC205345

file-alt Citations

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