SV2B (NM_014848) Human Tagged ORF Clone

SKU RG205345

SV2B (tGFP-tagged) - Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SV2B
• Synonyms: HsT19680
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205345 representing NM_014848; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATGACTACAAGTATCAGGACAATTATGGGGGCTATGCTCCCAGTGATGGCTATTACCGCGGCAATG; AGTCCAACCCAGAAGAAGATGCACAGAGTGATGTCACCGAAGGCCATGATGAGGAAGACGAGATCTATGA; GGGCGAGTACCAGGGTATCCCTCACCCAGATGATGTCAAGGCCAAGCAGGCCAAGATGGCGCCCTCCAGA; ATGGACAGCCTTCGGGGCCAGACAGACCTGATGGCTGAGAGGCTGGAAGATGAGGAGCAGTTGGCCCATC; AGTACGAGACCATCATGGATGAGTGTGGCCATGGCCGCTTCCAGTGGATCCTCTTTTTCGTCTTGGGTTT; GGCCCTGATGGCCGATGGGGTGGAAGTGTTCGTGGTGAGTTTTGCCCTGCCCAGTGCAGAGAAGGACATG; TGTCTGTCCAGTTCCAAAAAAGGAATGCTAGGGATGATAGTCTACTTGGGAATGATGGCGGGCGCCTTCA; TCCTGGGAGGCCTGGCTGATAAGCTGGGAAGGAAGCGAGTCCTCAGCATGTCTCTGGCCGTCAATGCCTC; CTTCGCCTCCCTCTCTTCCTTCGTGCAGGGATATGGAGCCTTCCTCTTCTGCCGACTCATCTCAGGCATC; GGTATTGGGGGTGCTCTACCGATTGTTTTTGCCTATTTTTCTGAATTCTTGTCTCGGGAGAAGCGAGGAG; AACACCTCAGTTGGCTGGGCATCTTCTGGATGACTGGGGGCCTGTACGCATCTGCCATGGCCTGGAGCAT; CATCCCACACTATGGCTGGGGCTTCAGCATGGGGACCAATTACCACTTCCATAGCTGGAGAGTGTTTGTC; ATCGTCTGTGCTCTGCCCTGCACCGTGTCCATGGTGGCCCTGAAGTTCATGCCAGAGAGCCCAAGGTTTC; TGCTAGAGATGGGCAAACATGATGAAGCCTGGATGATTCTCAAGCAAGTCCATGACACCAACATGAGAGC; TAAGGGGACCCCAGAGAAAGTGTTCACGGTTTCCAACATCAAAACTCCCAAGCAAATGGATGAATTCATT; GAGATCCAAAGTTCAACAGGAACCTGGTACCAGCGCTGGCTGGTCAGATTCAAGACCATTTTCAAGCAGG; TCTGGGATAATGCCCTGTACTGTGTGATGGGGCCCTACAGAATGAATACACTGATTCTGGCCGTGGTTTG; GTTTGCCATGGCATTCAGTTACTATGGACTGACAGTTTGGTTTCCTGATATGATCCGCTATTTTCAAGAT; GAAGAATACAAGTCTAAAATGAAGGTGTTTTTTGGTGAGCATGTGTACGGCGCCACAATCAACTTCACGA; TGGAAAATCAGATCCACCAACATGGGAAACTTGTGAATGATAAGTTCACAAGAATGTACTTTAAACATGT; ACTCTTTGAGGACACATTCTTTGACGAGTGCTATTTTGAAGACGTAACATCAACAGATACCTACTTCAAA; AATTGTACCATTGAATCAACCATCTTTTACAACACAGACCTCTACGAGCACAAGTTCATCAACTGTCGGT; TTATCAACTCCACCTTCCTGGAGCAGAAGGAGGGCTGCCACATGGACTTGGAGCAAGATAATGACTTCCT; GATTTACCTCGTCAGCTTCCTGGGCAGCCTGTCTGTCTTACCCGGGAACATCATTTCTGCCCTGCTCATG; GATAGAATTGGAAGGCTCAAGATGATTGGTGGCTCCATGCTAATCTCTGCAGTCTGCTGCTTCTTCCTGT; TTTTTGGCAACAGTGAGTCTGCAATGATCGGCTGGCAGTGCCTGTTCTGTGGGACAAGCATTGCAGCCTG; GAATGCTCTGGATGTGATCACAGTGGAGCTGTATCCCACCAACCAGAGAGCAACAGCCTTCGGCATTCTC; AATGGATTATGCAAATTTGGCGCCATCCTGGGAAACACCATCTTTGCTTCTTTTGTTGGGATAACCAAAG; TGGTCCCCATCCTTCTGGCTGCTGCTTCTCTGGTTGGGGGTGGCCTGATTGCCCTTCGACTGCCAGAGAC; TCGAGAACAGGTCCTGATG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205345 representing NM_014848; Red=Cloning site Green=Tags(s); ; MDDYKYQDNYGGYAPSDGYYRGNESNPEEDAQSDVTEGHDEEDEIYEGEYQGIPHPDDVKAKQAKMAPSR; MDSLRGQTDLMAERLEDEEQLAHQYETIMDECGHGRFQWILFFVLGLALMADGVEVFVVSFALPSAEKDM; CLSSSKKGMLGMIVYLGMMAGAFILGGLADKLGRKRVLSMSLAVNASFASLSSFVQGYGAFLFCRLISGI; GIGGALPIVFAYFSEFLSREKRGEHLSWLGIFWMTGGLYASAMAWSIIPHYGWGFSMGTNYHFHSWRVFV; IVCALPCTVSMVALKFMPESPRFLLEMGKHDEAWMILKQVHDTNMRAKGTPEKVFTVSNIKTPKQMDEFI; EIQSSTGTWYQRWLVRFKTIFKQVWDNALYCVMGPYRMNTLILAVVWFAMAFSYYGLTVWFPDMIRYFQD; EEYKSKMKVFFGEHVYGATINFTMENQIHQHGKLVNDKFTRMYFKHVLFEDTFFDECYFEDVTSTDTYFK; NCTIESTIFYNTDLYEHKFINCRFINSTFLEQKEGCHMDLEQDNDFLIYLVSFLGSLSVLPGNIISALLM; DRIGRLKMIGGSMLISAVCCFFLFFGNSESAMIGWQCLFCGTSIAAWNALDVITVELYPTNQRATAFGIL; NGLCKFGAILGNTIFASFVGITKVVPILLAAASLVGGGLIALRLPETREQVLM; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014848
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014848.2
• RefSeq Size: 5311 bp
• RefSeq ORF: 2052 bp
• Locus ID: 9899
• UniProt ID: Q7L1I2
• Cytogenetics: 15q26.1
• Domains: sugar_tr
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: ECM-receptor interaction
• Summary: This gene encodes a member of the synaptic vesicle proteins 2 (SV2) family and major facilitator superfamily of proteins. This protein and other members of the family are localized to synaptic vesicles and may function in the regulation of vesicle trafficking and exocytosis. Studies in mice suggest that the encoded protein may act as a protein receptor for botulinum neurotoxin E in neurons, and that this protein may be important for the integrity of the glomerular filtration barrier. This gene shows reduced expression in areas of synaptic loss in the hippocampus of human temporal lobe epilepsy patients. [provided by RefSeq, Sep 2016]