SV2B (NM_014848) Human Untagged Clone

SKU SC108749

SV2B (untagged)-Human synaptic vesicle glycoprotein 2B (SV2B), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SV2B
• Synonyms: HsT19680
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014848, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGATGACTACAAGTATCAGGACAATTATGGGGGCTATGCTCCCAGTGATGGCTATTACCGCGGCAATG; AGTCCAACCCAGAAGAAGATGCACAGAGTGATGTCACCGAAGGCCATGATGAGGAAGACGAGATCTATGA; GGGCGAGTACCAGGGTATCCCTCACCCAGATGATGTCAAGGCCAAGCAGGCCAAGATGGCGCCCTCCAGA; ATGGACAGCCTTCGGGGCCAGACAGACCTGATGGCTGAGAGGCTGGAAGATGAGGAGCAGTTGGCCCACC; AGTACGAGACCATCATGGATGAGTGTGGCCATGGCCGCTTCCAGTGGATCCTCTTTTTCGTCTTGGGTTT; GGCCCTGATGGCCGATGGGGTGGAAGTGTTCGTGGTGAGTTTTGCCCTGCCCAGTGCAGAGAAGGACATG; TGTCTGTCCAGTTCCAAAAAAGGAATGCTAGGGATGATAGTCTACTTGGGAATGATGGCGGGCGCCTTCA; TCCTGGGAGGCCTGGCTGATAAGCTGGGAAGGAAGCGAGTCCTCAGCATGTCTCTGGCCGTCAATGCCTC; CTTCGCCTCCCTCTCTTCCTTCGTGCAGGGATATGGAGCCTTCCTCTTCTGCCGACTCATCTCAGGCATC; GGTATTGGGGGTGCTCTACCGATTGTTTTTGCCTATTTTTCTGAATTCTTGTCTCGGGAGAAGCGAGGAG; AACACCTCAGTTGGCTGGGCATCTTCTGGATGACTGGGGGCCTGTACGCATCTGCCATGGCCTGGAGCAT; CATCCCACACTATGGCTGGGGCTTCAGCATGGGGACCAATTACCACTTCCATAGCTGGAGAGTGTTTGTC; ATCGTCTGTGCTCTGCCCTGCACCGTGTCCATGGTGGCCCTGAAGTTCATGCCAGAGAGCCCAAGGTTTC; TGCTAGAGATGGGCAAACATGATGAAGCCTGGATGATTCTCAAGCAAGTCCATGACACCAACATGAGAGC; TAAGGGGACCCCAGAGAAAGTGTTCACGGTTTCCAACATCAAAACTCCCAAGCAAATGGATGAATTCATT; GAGATCCAAAGTTCAACAGGAACCTGGTACCAGCGCTGGCTGGTCAGATTCAAGACCATTTTCAAGCAGG; TCTGGGATAATGCCCTGTACTGTGTGATGGGGCCCTACAGAATGAATACACTGATTCTGGCCGTGGTTTG; GTTTGCCATGGCATTCAGTTACTATGGACTGACAGTTTGGTTTCCTGATATGATCCGCTATTTTCAAGAT; GAAGAATACAAGTCTAAAATGAAGGTGTTTTTTGGTGAGCATGTGTACGGCGCCACAATCAACTTCACGA; TGGAAAATCAGATCCACCAACATGGGAAACTTGTGAATGATAAGTTCACAAGAATGTACTTTAAACATGT; ACTCTTTGAGGACACATTCTTTGACGAGTGCTATTTTGAAGACGTAACATCAACAGATACCTACTTCAAA; AATTGTACCATTGAATCAACCATCTTTTACAACACAGACCTCTACGAGCACAAGTTCATCAACTGTCGGT; TTATCAACTCCACCTTCCTGGAGCAGAAGGAGGGCTGCCACATGGACTTGGAGCAAGATAATGACTTCCT; GATTTACCTCGTCAGCTTCCTGGGCAGCCTGTCTGTCTTACCCGGGAACATCATTTCTGCCCTGCTCATG; GATAGAATTGGAAGGCTCAAGATGATTGGTGGCTCCATGCTAATCTCTGCAGTCTGCTGCTTCTTCCTGT; TTTTTGGCAACAGTGAGTCTGCAATGATCGGCTGGCAGTGCCTGTTCTGTGGGACAAGCATTGCAGCCTG; GAATGCTCTGGATGTGATCACAGTGGAGCTGTATCCCACCAACCAGAGAGCAACAGCCTTCGGCATTCTC; AATGGATTATGCAAATTTGGCGCCATCCTGGGAAACACCATCTTTGCTTCTTTTGTTGGGATAACCAAAG; TGGTCCCCATCCTTCTGGCTGCTGCTTCTCTGGTTGGGGGTGGCCTGATTGCCCTTCGACTGCCAGAGAC; TCGAGAACAGGTCCTGATGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014848 unedited; CAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGAAATAGCC; CAAACCTCTACCACAGAGCGAGGGATATAGCTCAAGGGGCAACCAGGCAGTCGCAGAACC; AAGGAATGGATGACTACAAGTATCAGGACAATTATGGGGGCTATGCTCCCAGTGATGGCT; ATTACCGCGGCAATGAGTCCAACCCAGAAGAAGATGCACAGAGTGATGTCACCGAAGGCC; ATGATGAGGAAGACGAGATCTATGAGGGCGAGTACCAGGGTATCCCTCACCCAGATGATG; TCAAGGCCAAGCAGGCCAAGATGGCGCCCTCCAGAATGGACAGCCTTCGGGGCCAGACAG; ACCTGATGGCTGAGAGGCTGGAAGATGAGGAGCAGTTGGCCCACCAGTACGAGACCATCA; TGGATGAGTGTGGCCATGGCCGCTTCCAGTGGATCCTCTTTTTCGTCTTGGGTTTGGCCC; TGATGGCCGATGGGGTGGAAGTGTTCGTGGTGAGTTTTGCCCTGCCCAGTGCAGAGAAGG; ACATGTGTCTGTCCAGTTCCAAAAAAGGAATGCTAGGGATGATAGTCTACTTGGGAATGA; TGGCGGGCGCCTTCATCCTGNGAGGCCTGGCTGATAAGCTGGGAAGGAAGCGAGTCCTCA; GCATGTCTCTGGCCGTCAATGCCTCCTTCGCCTCCCTCTCTTCCTTCGTGCAGGGATATG; GAGCCTTCCTCTTCTGCCGACTCATCTCANGCATCGGTATTGGNNGGTGCTCTACCGATT; GTTTTTGCCTATNTTTCTGAATTCTTGTCTCGGGAGAAGCGAGGAGACACCTCAGNTGGC; TGGGCATCTTCTGGATGACTGGGGGCCTGTACGCATCTGCCATGGCCTGNAGCATCATCC; ACACTATGGCTGGNGCTTAGCATGGGGACCAATACACTTCTACNTGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014848 unedited; TTTTAGCTTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTT; TTTCACTAATTATTTTTAATAAAACAAAAAATCAAAAATCAGCATCAGGAGATGACACAA; TACAGTTGAGACTATGGAAAGCAACTATTTCCAAGCTGGGCATCATAAAATACATCATCA; AGAACTGTGAACAGATTTGCTAAACAAAGGAAAGCCTCATCCCTTTAACCCTCCACAGCC; GACATCGGGAGTCTCTTGGGGTAAAACATGAAGTGAAAAAAAAGTGGATGGCCAAAATGC; TGTACATTGTCCCCCAGTGACCTGCCACAGAGACTATTTTTAAAGACGCATTTATCTCAG; CAGGAGTGACGGCATTTAAAAAGTAGCCCGGTACTCAGGCTAGACAATCCTGCAAAAGTC; TGCAAAGCTTGGCTGCACCCTTTACAAAGACCTGAGTGATGGATGAAATGGGAATTGCCC; TTGATTGGCTAGGTGGCTGGTTTAAAGAGCCATAAAGATAATGCAGAAGTGAGATCTTGG; AAACCAAATGGAGTTAGCCCCCAAATTATTGCAGCCACCCCAGCACTCCAATGTTAATCC; AATTTTAAATTTACAAGATCCATCTTAACTAGAAATGCAGGCTCTAACTGTCCTGCAAGT; TTTTTAAGTGGTAGCTGCTCAATGAATTGTATTATCCCATTAAAGAATAATGTTTTAGAT; GTGGTAATCCCAGACTCACACAATTCTCTTTTATGAACCTAGCCACTGTATTCATATGGA; AAGTAAATTACAGCTATATTACATCGAGCCTTTCANATTGACAGAGCATTAATGGTAGTA; CTCCTATAAACACTGAGAGAAGATTATACCCAGTGTTATATATATTTGAAGGAATAGGNT; GTCCAGTCAGACCAATAGTCTTCTCTCCCTGACAGAAAAAGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_014848
• Insert Size: 5700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014848.1, NP_055663.1
• RefSeq Size: 11361 bp
• RefSeq ORF: 11361 bp
• Locus ID: 9899
• UniProt ID: Q7L1I2
• Cytogenetics: 15q26.1
• Domains: sugar_tr
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: ECM-receptor interaction
• Summary: This gene encodes a member of the synaptic vesicle proteins 2 (SV2) family and major facilitator superfamily of proteins. This protein and other members of the family are localized to synaptic vesicles and may function in the regulation of vesicle trafficking and exocytosis. Studies in mice suggest that the encoded protein may act as a protein receptor for botulinum neurotoxin E in neurons, and that this protein may be important for the integrity of the glomerular filtration barrier. This gene shows reduced expression in areas of synaptic loss in the hippocampus of human temporal lobe epilepsy patients. [provided by RefSeq, Sep 2016]; Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.