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POU3F2 (NM_005604) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC205330
POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $732.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POU3F2
Synonyms brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POUF3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005604
ORF Size 1329 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005604.4
RefSeq Size 4172 bp
RefSeq ORF 1332 bp
Locus ID 5454
UniProt ID P20265
Cytogenetics 6q16.1
Domains homeobox, POU
Protein Families Transcription Factors
MW 46.7 kDa
Summary This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. provided by RefSeq, Mar 2012
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"NM_005604" in other vectors (6)
SKU Description Size Price
RC205330L1 Lenti-ORF clone of POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L2 Lenti-ORF clone of POU3F2 (mGFP-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L3 Lenti-ORF clone of POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L4 Lenti-ORF clone of POU3F2 (mGFP-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RG205330 POU3F2 (tGFP-tagged) - Human POU class 3 homeobox 2 (POU3F2) 10 ug
$932.00
SC116625 POU3F2 (untagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$686.00

Plasmid Map for RC205330

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file-download TXT (FASTA format)

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Cloning scheme for RC205330
Circular map for RC205330

file-alt Citations

Resistance to neoadjuvant talazoparib in triple-negative breast cancer by BRN2-induced ATR/STAT3 pathways or SHLD2 subclone expansion
Abdulkareem, Jiang, Qi et al
Proc Natl Acad Sci U S A (2026) 123 (16), e2513742123 DOI: 10.1073/pnas.2513742123
Cited by 1
AI Summary: OriGene products, including lentiviruses encoding human BRN2 (Cat. No. RC205330) and BRN2 shRNAs (Cat. No. TRCN0000019329 and TRCN0000019332), were used in this study to manipulate BRN2 expression and investigate its role in PARP inhibitor resistance in triple-negative breast cancer.
Snippet: mlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="pmc:entrez-nucleotide" xlink:href="PS100092">PS100092) and lentiviruses encoding human BRN2 (Cat. No. RC205330) were purchased from OriGene. For knockdown experiments, lentivirus containing BRN2 shRNAs (Cat. No. TRCN0000019329, shRNA #1;and Cat. No. TRCN0000019332, shRNA #2) and a nontargeting control (Cat. No. SHC007) were obtai
Showing 1-1 of 1 papers.
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