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POU3F2 (NM_005604) Human Untagged Clone

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SKU
SC116625
POU3F2 (untagged)-Human POU class 3 homeobox 2 (POU3F2)
  $686.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol POU3F2
Synonyms brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POUF3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_005604, the custom clone sequence may differ by one or more nucleotides


ATGGCGACCGCAGCGTCTAACCACTACAGCCTGCTCACCTCCAGCGCCTCCATCGTGCACGCCGAGCCGC
CCGGCGGCATGCAGCAGGGCGCGGGGGGCTACCGCGAAGCGCAGAGCCTGGTGCAGGGCGACTACGGCGC
TCTGCAGAGCAACGGACACCCGCTCAGCCACGCTCACCAGTGGATCACCGCGCTGTCCCACGGCGGCGGC
GGCGGGGGCGGTGGCGGCGGCGGGGGGGGCGGGGGCGGCGGCGGGGGCGGCGGCGACGGCTCCCCGTGGT
CCACCAGCCCCCTGGGCCAGCCGGACATCAAGCCCTCGGTGGTGGTGCAGCAGGGCGGCCGCGGAGACGA
GCTGCACGGGCCAGGCGCCCTGCAGCAGCAGCATCAGCAGCAGCAACAGCAACAGCAGCAGCAACAGCAG
CAACAGCAGCAGCAGCAGCAGCAACAGCGGCCGCCGCATCTGGTGCACCACGCCGCTAACCACCACCCGG
GACCCGGGGCATGGCGGAGCGCGGCGGCTGCAGCGCACCTCCCACCCTCCATGGGAGCGTCCAACGGCGG
CTTGCTCTACTCGCAGCCCAGCTTCACGGTGAACGGCATGCTGGGCGCCGGCGGGCAGCCGGCCGGTCTG
CACCACCACGGCCTGCGGGACGCGCACGACGAGCCACACCATGCCGACCACCACCCGCACCCGCACTCGC
ACCCACACCAGCAGCCGCCGCCCCCGCCGCCCCCGCAGGGTCCGCCTGGCCACCCAGGCGCGCACCACGA
CCCGCACTCGGACGAGGACACGCCGACCTCGGACGACCTGGAGCAGTTCGCCAAGCAGTTCAAGCAGCGG
CGGATCAAACTGGGATTTACCCAAGCGGACGTGGGGCTGGCTCTGGGCACCCTGTATGGCAACGTGTTCT
CGCAGACCACCATCTGCAGGTTTGAGGCCCTGCAGCTGAGCTTCAAGAACATGTGCAAGCTGAAGCCTTT
GTTGAACAAGTGGTTGGAGGAGGCGGACTCGTCCTCGGGCAGCCCCACGAGCATAGACAAGATCGCAGCG
CAAGGGCGCAAGCGGAAAAAGCGGACCTCCATCGAGGTGAGCGTCAAGGGGGCTCTGGAGAGCCATTTCC
TCAAATGCCCCAAGCCCTCGGCCCAGGAGATCACCTCCCTCGCGGACAGCTTACAGCTGGAGAAGGAGGT
GGTGAGAGTTTGGTTTTGTAACAGGAGACAGAAAGAGAAAAGGATGACCCCTCCCGGAGGGACTCTGCCG
GGCGCCGAGGATGTGTACGGGGGGAGTAGGGACACTCCACCACACCACGGGGTGCAGACGCCCGTCCAGT
GA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_005604 unedited
NATTATGTNATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGAGAGGGGGAGC
GCCGAGCTAGTCAGAGAGTGAGCGAGAGCGAGAAGGAGGGAGAGGAGGAGAAAGAGAGCG
AGGGCGGGCGGGAGGCGGCGGCGGCGGCAGCAGCAGCAGTAATAGCAGGAGCAGCAACAG
AAGGCGTCGGAGCGGGCGTCGGAGCTGCCCGCTGTGGGAGAGAGAGGAGACAGAAAGAGC
GAGCGAGGAGAGGGAGCCCGAGGCGAAAAAGTAACTGTCAAATGCGCGGCTCCTTTAACC
GGAGCGCTCAGTCCGGCTCCGAGAGTCATGGCGACCGCAGCGTCTAACCACTACAGCCTG
CTCACCTCCAGCGCCTCCATCGTGCACGCCGAGCCGCCCGGCGGCATGCAGCAGGGCGCG
GGGGGCTACCGCGAAGCGCAGAGCCTGGTGCAGGGCGACTACGGCGCTCTGCAGAGCAAC
GGACACCCGCTCAGCCACGCTCACCAGTGGATCACCGCGCTGTCCCACGGCGGCGGCGGC
GGGGGCGGTGGCGGCGGCGGGGGGGCCGGTCCTCTTTTCTCTCTCTATTCTCATCTCTTC
CCGCATTTCCCACCCCCCCCCCCCCTCTATTTTTTCTCTCTTCCAGCGCCCCTCCTCCTC
TCACTACCTTCTTCCCTCTCCCTCCTTTTCTTTTTTCCCCTTCACTTATTATAACACCCC
TCTCCCCCCCTCCCTTCTAACTATTTCTCCCCTCCCTCTCTATCCTCGCACCCCTCCCCA
CCCCTCCACTCGTATCTCCCTTCCTCTCGCTGTTATTTATTCCTTCTATTGTACAATCAT
CTCTACTCTTCTATTCCTTTCTTTCATCTCCCACCCANTTTTACTCTTGGTTCCGCCCAC
GCCCCCTCGCTCTCCTTCCTCTCTTATTTCTCCCTCTACTCCCCACCTATCTCTTCCTTC
CCCGCTTATCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005604 unedited
TATGGACCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATAAGATCTCT
GCGTTTTTATTTTCCCCACAAGCACAACCAAATGTACCAAAACAAAGTATTTTTTAAGAG
ACTGAACAAAAAAATTACATCCCATATAGATTTTGCTCACCTATTCATTTAAAGCTACAG
AAAAACAGTTTCCAGAACCTTTTTGTTTCAAAAGAAATAAATATACATTGAGGCAGGCCA
CTTAAAAATGATATGAAAATATTTCCCTGGGTAGCATTAAAAAAAAAGAAAAATAGACAA
AGAAACATTTNNAAACTATTTTNCTGGCATTCTTCAAAAGACAGATATCTAACATATATA
CATAAGTGGTAAAGGTTCAAGTGGGTAACTTTTCATTTCTTAGTAAACCCTATTNAGAGC
CAGGAGAAAGCTGTTAGACACATTTCTCTTGGAACTCCCAACCTTTTTGGAACCTTTACC
CTTAACTTGGGGGAATAAAAGGATATTCTGGGGGAAAATTCAATTTTCCCTCCCCTTTAA
CCAAAGGGGTATTTTTGGCCCCTGCGCTTGGGGAAAAGCCTTCCCCTTAAAGGGGCGCCA
TTTCCCCCTTCCCCCCTTTGGGNNNNNNNAAGCCNAACGGGTNTNCCACCCCGGAAANAN
NNTCTCCNGGGGCCACATTTTCCTTTGGGGAGACATTTTTTGTGGGGGGGGAAAGGGAAA
AAACCCGCGCGGAATTAATATTTGGCCCCCCTTATAAAGGGAACCCCCTTTTTTTCTTGG
GGCGAAAACAGCGGGAGAGAGGAAGAAACCCCCCCGGGGTCGTGTGAGTAAGAAGCATAT
TTTTAGGGCTTTTTTTTTCTCTAAAACCCTTTAAAGAACCTCGCGGGGGGGGAGCGCTGA
TTNTGTGGTCGCGGCACCCACAATTTTTTGGTGGGGGGGGGGACTCTCTCTTTTTCTTTA
TATAAAAAAAAATATCTCTTTCCCCCCCCCCGGGCGGGAAAAAACCCCCCCCCCTCTTCT
TTTAAA
Restriction Sites NotI-NotI |
ACCN NM_005604
Insert Size 3410 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation A trueClone.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005604.2, NP_005595.2
RefSeq Size 4108 bp
RefSeq ORF 1332 bp
Locus ID 5454
UniProt ID P20265
Cytogenetics 6q16.1
Domains homeobox, POU
Protein Families Transcription Factors
Summary This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. provided by RefSeq, Mar 2012
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Documents
Resources
"NM_005604" in other vectors (6)
SKU Description Size Price
RC205330 POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$732.00
RC205330L1 Lenti-ORF clone of POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L2 Lenti-ORF clone of POU3F2 (mGFP-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L3 Lenti-ORF clone of POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L4 Lenti-ORF clone of POU3F2 (mGFP-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RG205330 POU3F2 (tGFP-tagged) - Human POU class 3 homeobox 2 (POU3F2) 10 ug
$932.00

file-alt Citations

Targeted search for genetic causes of the Prader Willi like phenotype Gerichte zoektocht naar genetische oorzaken van het Prader Willi like fenotype
Geets, Aerts, Verrijken et al
Book (2018)
Snippet: Primer sequences for HRM and sequencing are available upon request. 2.4. POU3F2 construct and mutagenesis Wild type (WT) human POU3F2 (Genbank NM_005604. 2) cloned into a pCMV6-XL4 vector was obtained from Origene Technologies (SC116625) View as HTML
Showing 1-1 of 1 papers.
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