POU3F2 (NM_005604) Human Tagged ORF Clone

  • TrueORF®
SKU
RG205330
POU3F2 (tGFP-tagged) - Human POU class 3 homeobox 2 (POU3F2)
  $932.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POU3F2
Synonyms brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POUF3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205330 representing NM_005604
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCGCAGCGTCTAACCACTACAGCCTGCTCACCTCCAGCGCCTCCATCGTGCACGCCGAGCCGC
CCGGCGGCATGCAGCAGGGCGCGGGGGGCTACCGCGAAGCGCAGAGCCTGGTGCAGGGCGACTACGGCGC
TCTGCAGAGCAACGGACACCCGCTCAGCCACGCTCACCAGTGGATCACAGCGCTGTCCCACGGCGGCGGC
GGCGGGGGCGGTGGCGGCGGCGGGGGGGGCGGGGGCGGCGGCGGGGGCGGCGGCGACGGCTCCCCGTGGT
CCACCAGCCCCCTGGGCCAGCCGGACATCAAGCCCTCGGTGGTGGTGCAGCAGGGCGGCCGCGGAGACGA
GCTGCACGGGCCAGGCGCCCTGCAGCAGCAGCATCAGCAGCAGCAACAGCAACAGCAGCAGCAACAGCAG
CAACAGCAGCAGCAGCAGCAGCAACAGCGGCCGCCGCATCTGGTGCACCACGCCGCTAACCACCACCCGG
GACCCGGGGCATGGCGGAGCGCGGCGGCTGCAGCGCACCTCCCACCCTCCATGGGAGCGTCCAACGGCGG
CTTGCTCTACTCGCAGCCCAGCTTCACGGTGAACGGCATGCTGGGCGCCGGCGGGCAGTCGGCCGGGCTG
CACCACCACGGCCTGCGGGACGCGCACGACGAGCCACACCATGCCGACCACCACCCGCACCCGCACTCGC
ACCCACACCAGCAGCCGCCGCCCCCGCCGCCCCCGCAGGGTCCGCCTGGCCACCCAGGCGCGCACCACGA
CCCGCACTCGGACGAGGACACGCCGACCTCGGACGACCTGGAGCAGTTCGCCAAGCAGTTCAAGCAGCGG
CGGATCAAACTGGGATTTACACAAGCGGACGTGGGGCTGGCTCTGGGCACCCTGTATGGCAACGTGTTCT
CGCAGACCACCATCTGCAGGTTTGAGGCCCTGCAGCTGAGCTTCAAGAACATGTGCAAGCTGAAGCCTTT
GTTGAACAAGTGGTTGGAGGAGGCGGACTCGTCCTCGGGCAGCCCCACGAGCATAGACAAGATCGCAGCG
CAAGGGCGCAAGCGGAAAAAGCGGACCTCCATCGAGGTGAGCGTCAAGGGGGCTCTGGAGAGCCATTTCC
TCAAATGCCCCAAGCCCTCGGCCCAGGAGATCACCTCCCTCGCGGACAGCTTACAGCTGGAGAAGGAGGT
GGTGAGAGTTTGGTTTTGTAACAGGAGACAGAAAGAGAAAAGGATGACCCCTCCCGGAGGGACTCTGCCG
GGCGCCGAGGATGTGTACGGGGGGAGTAGGGACACTCCACCACACCACGGGGTGCAGACGCCCGTCCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205330 representing NM_005604
Red=Cloning site Green=Tags(s)

MATAASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQSLVQGDYGALQSNGHPLSHAHQWITALSHGGG
GGGGGGGGGGGGGGGGGGDGSPWSTSPLGQPDIKPSVVVQQGGRGDELHGPGALQQQHQQQQQQQQQQQQ
QQQQQQQQQRPPHLVHHAANHHPGPGAWRSAAAAAHLPPSMGASNGGLLYSQPSFTVNGMLGAGGQSAGL
HHHGLRDAHDEPHHADHHPHPHSHPHQQPPPPPPPQGPPGHPGAHHDPHSDEDTPTSDDLEQFAKQFKQR
RIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTSIDKIAA
QGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGGTLP
GAEDVYGGSRDTPPHHGVQTPVQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005604
ORF Size 1329 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005604.2, NP_005595.2
RefSeq Size 4172 bp
RefSeq ORF 1332 bp
Locus ID 5454
UniProt ID P20265
Cytogenetics 6q16.1
Domains homeobox, POU
Protein Families Transcription Factors
Summary This gene encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an increase in the proliferation of melanoma cells. provided by RefSeq, Mar 2012
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Documents
Resources
"NM_005604" in other vectors (6)
SKU Description Size Price
RC205330 POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$732.00
RC205330L1 Lenti-ORF clone of POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L2 Lenti-ORF clone of POU3F2 (mGFP-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L3 Lenti-ORF clone of POU3F2 (Myc-DDK-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
RC205330L4 Lenti-ORF clone of POU3F2 (mGFP-tagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$1,032.00
SC116625 POU3F2 (untagged)-Human POU class 3 homeobox 2 (POU3F2) 10 ug
$686.00

Plasmid Map for RG205330

Download sequence for

Cloning scheme for RG205330

Circular map for RG205330

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