DUSP1 (NM_004417) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DUSP1 |
| Synonyms | CL100; HVH1; MKP-1; MKP1; PTPN10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004417 |
| ORF Size | 1101 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004417.4 |
| RefSeq Size | 2015 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 1843 |
| UniProt ID | P28562, B4DU40 |
| Cytogenetics | 5q35.1 |
| Domains | DSPc, PTPc_motif, RHOD |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | MAPK signaling pathway |
| MW | 39.1 kDa |
| Summary | The protein encoded by this gene is a phosphatase with dual specificity for tyrosine and threonine. The encoded protein can dephosphorylate MAP kinase MAPK1/ERK2, which results in its involvement in several cellular processes. This protein appears to play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. Finally, the encoded protein can make some solid tumors resistant to both chemotherapy and radiotherapy, making it a target for cancer therapy. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004417" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205220L1 | Lenti ORF clone of Human dual specificity phosphatase 1 (DUSP1), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC205220L2 | Lenti ORF clone of Human dual specificity phosphatase 1 (DUSP1), mGFP tagged | 10 ug |
$757.00
|
|
| RC205220L3 | Lenti ORF clone of Human dual specificity phosphatase 1 (DUSP1), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC205220L4 | Lenti ORF clone of Human dual specificity phosphatase 1 (DUSP1), mGFP tagged | 10 ug |
$757.00
|
|
| RG205220 | DUSP1 (tGFP-tagged) - Human dual specificity phosphatase 1 (DUSP1) | 10 ug |
$489.00
$657.00
|
|
| SC117410 | DUSP1 (untagged)-Human dual specificity phosphatase 1 (DUSP1) | 10 ug |
$457.00
|
|
Citations
PDGF-R inhibition induces glioblastoma cell differentiation via DUSP1/p38MAPK signalling
Lane, Cilibrasi, Chen et al
Oncogene (2022) 41 (19), 2749-2763 DOI: 10.1038/s41388-022-02294-x
Lane, Cilibrasi, Chen et al
Oncogene (2022) 41 (19), 2749-2763 DOI: 10.1038/s41388-022-02294-x
Cited by 48
AI Summary: In this study, the OriGene DUSP1 plasmid (RC205220) was used to overexpress DUSP1 in glioblastoma cells, enabling the authors to investigate the role of DUSP1 in PDGF-R inhibition-induced differentiation.
Snippet: AGTTGTATGTTTGCTGATTA-3′ Qiagen Control siRNA Annealed, double stranded Qiagen DUSP1 overexpression Plasmid transfection was performed with 1 ng/µl pCMV6 empty vector or DUSP1 plasmid (Origene RC205220) for 48 h using transfection reagent HiPerFect. … Xenograft model in vivo Fo;lasmid transfection was performed with 1 ng/µl pCMV6 empty vector or DUSP1 plasmid (Origene RC205220) for 48 h using transfection reagent HiPerFect. …
Activation of AMPKα2 in adipocytes is essential for nicotine-induced insulin resistance in vivo
Wu, Song, Zhang et al
Nat. Med. (2015) 21 (4), 373-82 DOI: 10.1038/nm.3826
Wu, Song, Zhang et al
Nat. Med. (2015) 21 (4), 373-82 DOI: 10.1038/nm.3826
Cited by 170
AI Summary: OriGene contributed to this study by providing the wild-type MKP1 cDNA, which was used to generate DNA constructs for investigating the molecular mechanisms underlying nicotine-induced insulin resistance.
Snippet: Generation of DNA constructs. Wild-type MKP1 cDNA was purchased from Origene Company. S131, S334, or S359 of MKP1 was mutated to alanine using the QuikChange kit (Stratagene), according to the manufacturer’s instructions20,66. Oligonucleotides used for point mutation are listed in Supplementary Table 2. All mutations were confirmed by DNA sequencing. Plasmid DNA was extracted on a large scale usin...
Showing 1-2 of 2 papers.
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