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EIF3S2 (EIF3I) (NM_003757) Human Tagged ORF Clone

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SKU
RC201833
EIF3I (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF3S2
Synonyms eIF3-beta; eIF3-p36; EIF3S2; PRO2242; TRIP-1; TRIP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201833 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTATAACCGCGAAGGAGACC
TCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTACTCTGTGAATGGTGAGAGGCTGGGCAC
CTACATGGGCCATACCGGAGCTGTGTGGTGTGTGGACGCTGACTGGGACACCAAGCATGTCCTCACTGGC
TCAGCTGACAACAGCTGTCGTCTCTGGGACTGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATT
CGGCTGTCCGGACCTGCGGTTTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGG
CTACCAGTGCTTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCCCTACATG
AAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTTGGGGACCCCTGGGGGAGTGCATCATCGCTG
GCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGGAGAGGTGTTGGTGAATGTTAAGGAGCA
CTCCCGGCAGATCAACGACATCCAGTTATCCAGGGACATGACCATGTTTGTGACCGCGTCCAAGGACAAC
ACAGCCAAGCTTTTTGACTCCACAACTCTTGAACATCAGAAGACTTTCCGGACAGAACGTCCTGTCAACT
CAGCTGCCCTCTCCCCCAACTATGACCATGTGGTCCTGGGCGGTGGTCAGGAAGCCATGGATGTAACCAC
AACCTCCACCAGGATTGGCAAGTTTGAGGCCAGGTTCTTCCATTTGGCCTTTGAAGAAGAGTTTGGAAGA
GTCAAGGGTCACTTTGGACCTATCAACAGTGTTGCCTTCCATCCTGATGGCAAGAGCTACAGCAGCGGCG
GCGAAGATGGTTACGTCCGTATCCATTACTTCGACCCACAGTACTTCGAATTTGAGTTTGAGGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201833 protein sequence
Red=Cloning site Green=Tags(s)

MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTG
SADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDNNEPYM
KIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDN
TAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGR
VKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003757
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003757.3
RefSeq Size 1458 bp
RefSeq ORF 978 bp
Locus ID 8668
UniProt ID Q13347, Q5U0F4
Cytogenetics 1p35.2
Domains WD40
MW 36.5 kDa
Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniProtKB/Swiss-Prot Function
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"NM_003757" in other vectors (7)
SKU Description Size Price
RC201833L1 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), Myc-DDK-tagged 10 ug
$600.00
RC201833L2 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), mGFP tagged 10 ug
$600.00
RC201833L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), Myc-DDK-tagged 10 ug
$600.00
RC201833L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), mGFP tagged 10 ug
$600.00
RG201833 EIF3I (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit I (EIF3I) 10 ug
$489.00 $500.00
SC117777 EIF3I (untagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I) 10 ug
$300.00
SC320585 EIF3I (untagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I) 10 ug
$300.00

Plasmid Map for RC201833

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201833
Circular map for RC201833

file-alt Citations

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