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EIF3S2 (EIF3I) (NM_003757) Human Untagged Clone

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SKU
SC117777
EIF3I (untagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EIF3S2
Synonyms eIF3-beta; eIF3-p36; EIF3S2; PRO2242; TRIP-1; TRIP1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC117777 sequence for NM_003757 edited (data generated by NextGen Sequencing)
ATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTATAACCGC
GAAGGAGACCTCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTACTCTGTG
AATGGTGAGAGGCTGGGCACCTACATGGGCCATACCGGAGCTGTGTGGTGTGTGGACGCT
GACTGGGACACCAAGCATGTCCTCACTGGCTCAGCTGACAACAGCTGTCGTCTCTGGGAC
TGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATTCGGCTGTCCGGACCTGCGGT
TTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGGCTACCAGTGC
TTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCCCTACATG
AAGATCCCTTGCAATGACTCTAAAATCACCAGTGCTGTTTGGGGACCCCTGGGGGAGTGC
ATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGGAGAGGTG
TTGGTGAATGTTAAGGAGCACTCCCGGCAGATCAACGACATCCAGTTATCCAGGGACATG
ACCATGTTTGTGACCGCGTCCAAGGACAACACAGCCAAGCTTTTTGACTCCACAACTCTT
GAACATCAGAAGACTTTCCGGACAGAACGTCCTGTCAACTCAGCTGCCCTCTCCCCCAAC
TATGACCATGTGGTCCTGGGCGGTGGTCAGGAAGCCATGGATGTAACCACAACCTCCACC
AGGATTGGCAAGTTTGAGGCCAGGTTCTTCCATTTGGCCTTTGAAGAAGAGTTTGGAAGA
GTCAAGGGTCACTTTGGACCTATCAACAGTGTTGCCTTCCATCCTGATGGCAAGAGCTAC
AGCAGCGGCGGCGAAGATGGTTACGTCCGTATCCATTACTTCGACCCACAGTACTTCGAA
TTTGAGTTTGAGGCTTAA

Clone variation with respect to NM_003757.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003757 unedited
TCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCACGTTGCGGCCTTCCTCGCGTCAC
AGCCGGGATGAAGCCGATCCTACTGCAGGGCCATGAGCGGTCCATTACGCAGATTAAGTA
TAACCGCGAAGGAGACCTCCTCTTTACTGTGGCCAAGGACCCTATCGTCAATGTATGGTA
CTCTGTGAATGGTGAGAGGCTGGGCACCTACATGGGCCATACCGGAGCTGTGTGGTGTGT
GGACGCTGACTGGGACACCAAGCATGTCCTCACTGGCTCAGCTGACAACAGCTGTCGTCT
CTGGGACTGTGAAACAGGAAAGCAGCTGGCCCTTCTCAAGACCAATTCGGCTGTCCGGAC
CTGCGGTTTTGACTTTGGGGGCAACATCATCATGTTCTCCACGGACAAGCAGATGGGCTA
CCAGTGCTTTGTGAGCTTTTTTGACCTGCGGGATCCGAGCCAGATTGACAACAATGAGCC
CTACATGAAGATCCCTTGNCATGACTCTAANATCACCAGTGCTGTTTGGGGACCCCTGGG
GGAGTGCATCATCGCTGGCCATGAGAGTGGAGAGCTCAACCAGTATAGTGCCAAGTCTGG
AGAGGTGTTGTGAATGTTAAGAGCACTCCCGCAGATCACGACACCAGTATCCAGGACATG
ACATGTTGTGACCGCGTCAAGACACACAGCCAGCTTTTTGACNCACAACTCTTGACATAN
AAGACTTCCGGACAGACGTTCTGTCACTAGCTGCCCTCTCCCCAACTATGACATGTGTCC
TGGGCCGTGGTCNGAANCCATGGATGTACCACCACCTCCCCAGATTGCCAGTTGAAGGCA
GTTCTCCTTTTGCCTTGAAAAAAGTTGAAAATAAGGTCCTTTGGACTATCAAGGGTGCCT
CCTCTGAGCAAACTCACACGCGGAAAATGTACNTCGATCATATTGACCAGACTTGATGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003757 unedited
NNGGTAACTCTTGAACCGCGCCGCAATCTANGATCGATTTTTTTTTTTTTTTTTTGAGAC
GGAGTTTTGCTCTTGTTGCTCAGGGTGGAGTGCAATGACGTGATCTCAGCTCACTGCAAC
CTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTATA
GGCGTGTGCCACCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGGTTTCTCCAT
GTTGGTCAGGCTGGTCTTAAACTCCTGACCTCAGGTGATCCGCCTGCCTTAAAAAAAAAG
TGGTGGGATTACAGGCATGAGCCACCACGCCCGGCAGGAGATCCAGCTTCTTAAGCCTCA
AACTCAAATTCGAAGTACTGTGGGTCGAAGTAATGGATACGGACGTAACCATCTTCGCCG
CCGCTGCTGTAGCTCTTGCCATCAGGATGGAAGGCAACACTGTTGATAGGTCCAAAGTGA
CCCTTGACTCTTCCAAACTCTTCTTCAAAGGCCAAATGGAAGAACCTGGCCTCAAACTTG
CCAATCCTGGTGGAGGTTGTGGTTACATCCATGGCTTCCTGACCACCGCCCAGGACCACA
TGGTCATAGTTGGGGGAGAGGGCAGCTGAGTTGACAGGACGTTCTGTCCGGAAAGTCTTC
TGATGTTCAAGAGTTGTGGAGTCAAAAAGCTTGCCTGTGTTGTCCTTGGACGCGGTCACA
AACATGGGCATGTCCCTGGATAACTGGATGTCGTTGATCTGCCCGGAGTGCTCCCTACAT
TCACCAACACCTCTTCAGACTTGGCACTATCCTGGTTGACCTCTCACCTCCATGGCCAAC
GATGATGCACTCCCCCAGGGTCCCCAAAACCACTGTTGATTTTAAACCC
Restriction Sites NotI-NotI |
ACCN NM_003757
Insert Size 1370 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003757.1, NP_003748.1
RefSeq Size 1402 bp
RefSeq ORF 978 bp
Locus ID 8668
UniProt ID Q13347
Cytogenetics 1p35.2
Domains WD40
Summary Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniProtKB/Swiss-Prot Function
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"NM_003757" in other vectors (7)
SKU Description Size Price
RC201833 EIF3I (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I) 10 ug
$300.00
RC201833L1 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), Myc-DDK-tagged 10 ug
$600.00
RC201833L2 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), mGFP tagged 10 ug
$600.00
RC201833L3 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), Myc-DDK-tagged 10 ug
$600.00
RC201833L4 Lenti ORF clone of Human eukaryotic translation initiation factor 3, subunit I (EIF3I), mGFP tagged 10 ug
$600.00
RG201833 EIF3I (tGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit I (EIF3I) 10 ug
$489.00 $500.00
SC320585 EIF3I (untagged)-Human eukaryotic translation initiation factor 3, subunit I (EIF3I) 10 ug
$300.00

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