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TTI1 (NM_014657) Human Tagged ORF Clone

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SKU
RC201622
TTI1 (Myc-DDK-tagged)-Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $948.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TTI1
Synonyms KIAA0406; smg-10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC201622 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGTTTTTGATACTCCTGAGGAGGCCTTTGGTGTCTTACGTCCAGTCTGTGTTCAGCTCACAAAGA
CCCAGACAGTGGAGAATGTGGAGCATCTGCAGACACGACTACAAGCTGTGAGTGACAGTGCCCTTCAGGA
ACTTCAGCAGTACATCCTCTTCCCTCTGCGATTTACCCTGAAGACCCCAGGTCCCAAAAGAGAGCGTTTG
ATCCAAAGTGTGGTGGAATGCCTCACATTTGTCCTTTCTTCAACATGTGTGAAAGAACAGGAGCTTCTCC
AGGAACTCTTTTCAGAACTCTCTGCTTGTCTGTATTCACCCAGCTCCCAAAAACCTGCGGCTGTGTCCGA
GGAGTTGAAATTGGCTGTGATCCAGGGACTTAGCACATTAATGCACTCAGCTTATGGGGACATCATTCTG
ACTTTTTATGAGCCCTCCATTCTGCCACGTTTAGGATTTGCTGTATCTTTACTGTTAGGCCTTGCAGAAC
AGGAGAAATCAAAGCAAATTAAAATTGCTGCCTTAAAATGTTTACAGGTTCTACTCTTGCAGTGTGATTG
TCAGGACCATCCAAGGTCATTGGATGAACTTGAACAAAAGCAGCTGGGGGATTTGTTTGCCTCTTTTTTA
CCTGGAATCTCAACTGCACTGACCAGGCTTATCACAGGAGACTTTAAACAAGGTCACAGCATTGTCGTAT
CTTCCCTAAAGATCTTTTACAAGACAGTGAGCTTCATTATGGCTGATGAACAGCTCAAAAGAATCTCAAA
GGTCCAAGCAAAACCTGCAGTTGAGCACAGAGTAGCAGAGCTGATGGTTTACAGGGAAGCAGATTGGGTA
AAAAAGACTGGCGACAAGTTGACTATCCTTATTAAAAAGATAATTGAGTGTGTTTCTGTTCACCCACACT
GGAAGGTGAGACTGGAACTGGTAGAACTTGTGGAGGACCTTCTTTTGAAGTGCAGTCAATCATTGGTCGA
ATGTGCTGGTCCCCTTCTGAAGGCCTTAGTGGGACTAGTAAATGATGAGAGTCCTGAAATCCAAGCCCAG
TGCAATAAAGTTCTGAGACATTTTGCAGATCAAAAAGTAGTGGTGGGCAACAAAGCCCTCGCTGACATCT
TGTCAGAAAGCCTGCATTCCCTTGCCACATCTCTTCCTCGCCTAATGAACTCCCAAGATGACCAGGGCAA
ATTCTCTACTCTTTCCTTGTTACTTGGTTATCTGAAACTCTTGGGCCCAAAAATAAACTTTGTCCTCAAC
TCTGTGGCCCATCTCCAGCGGCTTTCCAAAGCACTCATCCAAGTTCTAGAGCTAGACGTGGCTGACATCA
AGATTGTTGAGGAACGGCGTTGGAACTCTGATGATCTGAATGCTTCTCCAAAGACCTCAGCCACACAGCC
TTGGAACCGCATCCAGAGGAGATATTTCCGCTTCTTCACTGATGAGAGAATCTTCATGCTCTTGAGGCAG
GTTTGTCAGCTACTTGGTTATTATGGGAATCTTTATTTGCTTGTGGATCACTTTATGGAACTTTACCATC
AATCTGTGGTTTACCGGAAGCAAGCTGCCATGATCCTTAATGAACTGGTTACAGGGGCTGCTGGGCTGGA
GGTTGAGGATCTTCACGAAAAACATATTAAAACAAACCCAGAAGAACTGAGAGAGATTGTGACATCTATA
CTTGAAGAATACACAAGTCAAGAAAATTGGTATTTGGTTACCTGTCTTGAAACTGAGGAAATGGGAGAGG
AGCTGATGATGGAGCACCCAGGCCTCCAAGCCATCACGTCTGGTGAACACACCTGCCAAGTTACATCTTT
TCTAGCCTTCTCAAAGCCAAGTCCCACTATTTGCTCCATGAACAGTAACATCTGGCAAATATGCATTCAG
TTGGAAGGAATTGGCCAGTTTGCATATGCACTAGGAAAAGACTTCTGTTTGCTCTTGATGTCAGCCCTTT
ATCCAGTACTGGAGAAGGCTGGAGACCAAACCCTACTCATTAGTCAGGTGGCTACCAGCACCATGATGGA
CGTTTGCCGTGCTTGTGGCTACGACTCCCTGCAGCACCTGATCAATCAAAATTCAGACTATTTAGTGAAT
GGGATCTCTTTAAATCTGCGTCATCTGGCTCTGCATCCTCATACCCCAAAGGTCCTGGAAGTCATGCTGC
GGAACTCAGATGCTAACCTGCTTCCTTTGGTGGCAGATGTGGTTCAAGATGTCTTGGCCACCCTGGACCA
ATTTTACGATAAGAGAGCTGCTTCCTTTGTCAGCGTTCTGCATGCTCTGATGGCAGCATTAGCCCAGTGG
TTCCCAGACACAGGTAATCTTGGGCACCTCCAAGAGCAAAGTTTAGGAGAAGAGGGAAGTCATTTGAACC
AAAGACCAGCAGCTCTTGAGAAGAGCACCACCACAGCTGAAGACATCGAACAGTTTTTGCTGAACTACCT
CAAAGAGAAGGATGTGGCAGATGGAAATGTCTCGGATTTTGATAATGAAGAAGAGGAACAGTCAGTCCCT
CCCAAAGTGGATGAGAATGACACCCGTCCAGATGTGGAGCCACCACTGCCATTGCAGATCCAAATAGCCA
TGGACGTGATGGAACGCTGCATCCACTTGTTGTCAGATAAAAATCTGCAAATCCGCCTGAAGGTCTTGGA
TGTGCTGGATCTGTGTGTGGTTGTTCTTCAGTCCCACAAAAACCAGCTGCTTCCCTTGGCTCATCAGGCC
TGGCCCTCGCTCGTTCACCGACTCACACGGGACGCCCCCCTGGCAGTGCTTAGAGCCTTCAAGGTTTTAC
GTACCCTGGGAAGCAAGTGTGGTGACTTTCTTCGCAGCCGGTTCTGCAAAGATGTCCTGCCAAAGCTGGC
TGGCTCCCTAGTCACCCAGGCCCCCATCAGTGCCAGGGCTGGACCAGTTTACTCGCACACGCTGGCCTTC
AAGTTGCAGCTGGCTGTCTTACAGGGCCTGGGCCCCCTCTGTGAGAGACTGGACCTAGGTGAGGGTGACC
TGAATAAAGTGGCTGATGCCTGCTTGATTTACCTCAGTGTCAAACAGCCCGTGAAATTACAAGAGGCTGC
CAGGAGCGTCTTCCTCCACTTGATGAAGGTGGACCCAGACTCCACCTGGTTCCTCCTGAACGAGCTTTAC
TGCCCCGTGCAGTTCACACCTCCCCACCCCAGCCTCCACCCTGTGCAGCTGCACGGGGCCAGCGGGCAGC
AGAACCCCTACACGACCAACGTGCTCCAGCTGCTCAAGGAGCTGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC201622 protein sequence
Red=Cloning site Green=Tags(s)

MAVFDTPEEAFGVLRPVCVQLTKTQTVENVEHLQTRLQAVSDSALQELQQYILFPLRFTLKTPGPKRERL
IQSVVECLTFVLSSTCVKEQELLQELFSELSACLYSPSSQKPAAVSEELKLAVIQGLSTLMHSAYGDIIL
TFYEPSILPRLGFAVSLLLGLAEQEKSKQIKIAALKCLQVLLLQCDCQDHPRSLDELEQKQLGDLFASFL
PGISTALTRLITGDFKQGHSIVVSSLKIFYKTVSFIMADEQLKRISKVQAKPAVEHRVAELMVYREADWV
KKTGDKLTILIKKIIECVSVHPHWKVRLELVELVEDLLLKCSQSLVECAGPLLKALVGLVNDESPEIQAQ
CNKVLRHFADQKVVVGNKALADILSESLHSLATSLPRLMNSQDDQGKFSTLSLLLGYLKLLGPKINFVLN
SVAHLQRLSKALIQVLELDVADIKIVEERRWNSDDLNASPKTSATQPWNRIQRRYFRFFTDERIFMLLRQ
VCQLLGYYGNLYLLVDHFMELYHQSVVYRKQAAMILNELVTGAAGLEVEDLHEKHIKTNPEELREIVTSI
LEEYTSQENWYLVTCLETEEMGEELMMEHPGLQAITSGEHTCQVTSFLAFSKPSPTICSMNSNIWQICIQ
LEGIGQFAYALGKDFCLLLMSALYPVLEKAGDQTLLISQVATSTMMDVCRACGYDSLQHLINQNSDYLVN
GISLNLRHLALHPHTPKVLEVMLRNSDANLLPLVADVVQDVLATLDQFYDKRAASFVSVLHALMAALAQW
FPDTGNLGHLQEQSLGEEGSHLNQRPAALEKSTTTAEDIEQFLLNYLKEKDVADGNVSDFDNEEEEQSVP
PKVDENDTRPDVEPPLPLQIQIAMDVMERCIHLLSDKNLQIRLKVLDVLDLCVVVLQSHKNQLLPLAHQA
WPSLVHRLTRDAPLAVLRAFKVLRTLGSKCGDFLRSRFCKDVLPKLAGSLVTQAPISARAGPVYSHTLAF
KLQLAVLQGLGPLCERLDLGEGDLNKVADACLIYLSVKQPVKLQEAARSVFLHLMKVDPDSTWFLLNELY
CPVQFTPPHPSLHPVQLHGASGQQNPYTTNVLQLLKELQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014657
ORF Size 3267 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014657.3
RefSeq Size 3931 bp
RefSeq ORF 3270 bp
Locus ID 9675
UniProt ID O43156
Cytogenetics 20q11.23
MW 122.1 kDa
Summary Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014657" in other vectors (7)
SKU Description Size Price
RC201622L1 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), Myc-DDK-tagged 10 ug
$1,248.00
RC201622L2 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), mGFP tagged 10 ug
$1,248.00
RC201622L3 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), Myc-DDK-tagged 10 ug
$1,248.00
RC201622L4 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), mGFP tagged 10 ug
$1,248.00
RG201622 TTI1 (tGFP-tagged) - Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1) 10 ug
$789.00 $1,148.00
SC100424 TTI1 (untagged)-Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1) 10 ug
$949.00
SC324266 TTI1 (untagged)-Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1) 10 ug
$949.00

Plasmid Map for RC201622

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC201622
Circular map for RC201622

file-alt Citations

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