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TTI1 (NM_014657) Human Untagged Clone

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SKU
SC100424
TTI1 (untagged)-Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1)
  $949.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TTI1
Synonyms KIAA0406; smg-10
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC100424 sequence for NM_014657 edited (data generated by NextGen Sequencing)
ATGGCAGTTTTTGATACTCCTGAGGAGGCCTTTGGTGTCTTACGTCCAGTCTGTGTTCAG
CTCACAAAGACCCAGACAGTGGAGAATGTGGAGCATCTGCAGACACGACTACAAGCTGTG
AGTGACAGTGCCCTTCAGGAACTTCAGCAGTACATCCTCTTCCCTCTGCGATTTACCCTG
AAGACCCCAGGTCCCAAAAGAGAGCGTTTGATCCAAAGTGTGGTGGAATGCCTCACATTT
GTCCTTTCTTCAACATGTGTGAAAGAACAGGAGCTTCTCCAGGAACTCTTTTCAGAACTC
TCTGCTTGTCTGTATTCACCCAGCTCCCAAAAACCTGCGGCTGTGTCCGAGGAGTTGAAA
TTGGCTGTGATCCAGGGACTTAGCACATTAATGCACTCAGCTTATGGGGACATCATTCTG
ACTTTTTATGAGCCCTCCATTCTGCCACGTTTAGGATTTGCTGTATCTTTACTGTTAGGC
CTTGCAGAACAGGAGAAATCAAAGCAAATTAAAATTGCTGCCTTAAAATGTTTACAGGTT
CTACTCTTGCAGTGTGATTGTCAGGACCATCCAAGGTCATTGGATGAACTTGAACAAAAG
CAGCTGGGGGATTTGTTTGCCTCTTTTTTACCTGGAATCTCAACTGCACTGACCAGGCTT
ATCACAGGAGACTTTAAACAAGGTCACAGCATTGTCGTATCTTCCCTAAAGATCTTTTAC
AAGACAGTGAGCTTCATTATGGCTGATGAACAGCTCAAAAGAATCTCAAAGGTCCAAGCA
AAACCTGCAGTTGAGCACAGAGTAGCAGAGCTGATGGTTTACAGGGAAGCAGATTGGGTA
AAAAAGACTGGCGACAAGTTGACTATCCTTATTAAAAAGATAATTGAGTGTGTTTCTGTT
CACCCACACTGGAAGGTGAGACTGGAACTGGTAGAACTTGTGGAGGACCTTCTTTTGAAG
TGCAGTCAATCATTGGTCGAATGTGCTGGTCCCCTTCTGAAGGCCTTAGTGGGACTAGTA
AATGATGAGAGTCCTGAAATCCAAGCCCAGTGCAATAAAGTTCTGAGACATTTTGCAGAT
CAAAAAGTAGTGGTGGGCAACAAAGCCCTCGCTGACATCTTGTCAGAAAGCCTGCATTCC
CTTGCCACATCTCTTCCTCGCCTAATGAACTCCCAAGATGACCAGGGCAAATTCTCTACT
CTTTCCTTGTTACTTGGTTATCTGAAACTCTTGGGCCCAAAAATAAACTTTGTCCTCAAC
TCTGTGGCCCATCTCCAGCGGCTTTCCAAAGCACTCATCCAAGTTCTAGAGCTAGACGTG
GCTGACATCAAGATTGTTGAGGAACGGCGTTGGAACTCTGATGATCTGAATGCTTCTCCA
AAGACCTCAGCCACACAGCCTTGGAACCGCATCCAGAGGAGATATTTCCGCTTCTTCACT
GATGAGAGAATCTTCATGCTCTTGAGGCAGGTTTGTCAGCTACTTGGTTATTATGGGAAT
CTTTATTTGCTTGTGGATCACTTTATGGAACTTTACCATCAATCTGTGGTTTACCGGAAG
CAAGCTGCCATGATCCTTAATGAACTGGTTACAGGGGCTGCTGGGCTGGAGGTTGAGGAT
CTTCACGAAAAACATATTAAAACAAACCCAGAAGAACTGAGAGAGATTGTGACATCTATA
CTTGAAGAATACACAAGTCAAGAAAATTGGTATTTGGTTACCTGTCTTGAAACTGAGGAA
ATGGGAGAGGAGCTGATGATGGAGCACCCAGGCCTCCAAGCCATCACGTCTGGTGAACAC
ACCTGCCAAGTTACATCTTTTCTAGCCTTCTCAAAGCCAAGTCCCACTATTTGCTCCATG
AACAGTAACATCTGGCAAATATGCATTCAGTTGGAAGGAATTGGCCAGTTTGCATATGCA
CTAGGAAAAGACTTCTGTTTGCTCTTGATGTCAGCCCTTTATCCAGTACTGGAGAAGGCT
GGAGACCAAACCCTACTCATTAGTCAGGTGGCTACCAGCACCATGATGGACGTTTGCCGT
GCTTGTGGCTACGACTCCCTGCAGCACCTGATCAATCAAAATTCAGACTATTTAGTGAAT
GGGATCTCTTTAAATCTGCGTCATCTGGCTCTGCATCCTCATACCCCAAAGGTCCTGGAA
GTCATGCTGCGGAACTCAGATGCTAACCTGCTTCCTTTGGTGGCAGATGTGGTTCAAGAT
GTCTTGGCCACCCTGGACCAATTTTACGATAAGAGAGCTGCTTCCTTTGTCAGCGTTCTG
CATGCTCTGATGGCAGCATTAGCCCAGTGGTTCCCAGACACAGGTAATCTTGGGCACCTC
CAAGAGCAAAGTTTAGGAGAAGAGGGAAGTCATTTGAACCAAAGACCAGCAGCTCTTGAG
AAGAGCACCACCACAGCTGAAGACATCGAACAGTTTTTGCTGAACTACCTCAAAGAGAAG
GATGTGGCAGATGGAAATGTCTCGGATTTTGATAATGAAGAAGAGGAACAGTCAGTCCCT
CCCAAAGTGGATGAGAATGACACCCGTCCAGATGTGGAGCCACCACTGCCATTGCAGATC
CAAATAGCCATGGACGTGATGGAACGCTGCATCCACTTGTTGTCAGATAAAAATCTGCAA
ATCCGCCTGAAGGTCTTGGATGTGCTGGATCTGTGTGTGGTTGTTCTTCAGTCCCACAAA
AACCAGCTGCTTCCCTTGGCTCATCAGGCCTGGCCCTCGCTCGTTCACCGACTCACACGG
GACGCCCCCCTGGCAGTGCTTAGAGCCTTCAAGGTTTTACGTACCCTGGGAAGCAAGTGT
GGTGACTTTCTTCGCAGCCGGTTCTGCAAAGATGTCCTGCCAAAGCTGGCTGGCTCCCTA
GTCACCCAGGCCCCCATCAGTGCCAGGGCTGGACCAGTTTACTCGCACACGCTGGCCTTC
AAGTTGCAGCTGGCTGTCTTACAGGGCCTGGGCCCCCTCTGTGAGAGACTGGACCTAGGT
GAGGGTGACCTGAATAAAGTGGCTGATGCCTGCTTGATTTACCTCAGTGTCAAACAGCCC
GTGAAATTACAAGAGGCTGCCAGGAGCGTCTTCCTCCACTTGATGAAGGTGGACCCAGAC
TCCACCTGGTTCCTCCTGAACGAGCTTTACTGCCCCGTGCAGTTCACACCTCCCCACCCC
AGCCTCCACCCTGTGCAGCTGCACGGGGCCAGCGGGCAGCAGAACCCCTACACGACCAAC
GTGCTCCAGCTGCTCAAGGAGCTGCAGTGA

Clone variation with respect to NM_014657.1
5' Read Nucleotide Sequence
>OriGene 5' read for NM_014657 unedited
GGGTTGCAACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGAGAAAG
CTGGAAGACGAGCCTGCAGGATGTTTCCTCAATGAGGGGAAGGCTGCTGCACAATGGCAG
TTTTTGATACTCCTGAGGAGGCCTTTGGTGTCTTACGTCCAGTCTGTGTTCAGCTCACAA
AGACCCAGACAGTGGAGAATGTGGAGCATCTGCAGACACGACTACAAGCTGTGAGTGACA
GTGCCCTTCAGGAACTTCAGCAGTACATCCTCTTCCCTCTGCGATTTACCCTGAAGACCC
CAGGTCCCAAAAGAGAGCGTTTGATCCAAAGTGTGGTGGAATGCCTCACATTTGTCCTTT
CTTCAACATGTGTGAAAGAACAGGAGCTTCTCCAGGAACTCTTTTCAGAACTCTCTGCTT
GTCTGTATTCACCCAGCTCCCAAAAACCTGCGGCTGTGTCCGAGGAGTTGAAATTGGCTG
TGATCCAGGGACTTAGCACATTAATGCACTCAGCTTATGGGGACATCATTCTGACTTTTT
ATGAGCCCTCCATTCTGCCACGTTTAGGATTTGCTGTATCTTTACTGTTAGGCCTTGCAG
AACAGGAGAAATCAAAGCAAATTAAAATTGCTGCCTTAAAATGTTTACAGGTTCTACTCT
TGCAGTGTGATTGTCAGGACCATCCAAGGTCATTGGATGAACTTGAACAAAAGCAGCTGG
GGGATTTGTTTGCCTCTTTTTACCTGGAATCTCAACTGCACTGACCANGCTTATCACANG
AGACTTNTAACAAGGTCACAGCATTGTCGTATCTTCCCTAAGATCTTTTACAAGACGTGA
GCTTCATTATGGCTGATGAACAGCTCAAAAGGATCTCCAAGGTCCAAGCAAAACTGCAGN
TGAGCACAGAG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_014657 unedited
TGACCGCGGCCGCTTTTAGAGTCGAGTTTTTTTTTTTTTTTTTTTACTTTTCATCCAAAA
AGTGTCTTTATTTCCGTTTGTTTCCCAAAGACAGGTTAGGTAAAACAAATTAGTTCCATC
TCATTATTTGAAGACAGGGAGGTGTATGCAGATGGAGGGGAACTGACCTCTTGTGTGTGT
GTGTGTGTGGCCTGTGAGGGAGCTGGGGCTATAGAGATGGTAGGCTAAGGGGTTAAACCC
TTAGAGCCACCAGACAATGTCACTTGACAACACAAGGTATGAAACATAAATAATAGTCAG
CTACTTTCCTTCAATCCATTTCTAAGCAGTTGTGTGATCGATCAATTTATAAATCGATTG
GCTAACTAATTCACCTTCTCTGCTGCCGCTGCCACCGCCTATGGCCGGGGTGGGGTCAGC
CCAGCTTCTGGCTGGCAGTACGGGAGGGATCGGTGGCCTCTGTGGTGGGGGAGCAGGGTC
ACTGCAGCTCCTTGAGCAGCTGGAGCCACGTTGGTCGTGTAGGGGTTCTGCTGCCCGCTG
GCCCCGTGCAGCTGCACAGGGTGGAGGCTGGCGTGGGGAGGTGTGAACTGTACGGGGCAG
TAAAGCTCGTTCATGAGGAACCACGCGGAGTCTGGCTCCACTTCATCAAGTGGAGGAAGA
CCCTCCTGCCAGCCCTCTTGTATTTTTACGGCCTGTTTGACCCTGAGGTAAATCAAGCAG
GCCTCCCCCACTTTATTCAGGTCACCCTCCCCTAAGTCCAGCCTTTTCAAGACGGGGCCC
AGGCCCTGCAGGAATCCAGCTGCACCTTGAGGCCCACGGTCGCACTAACCGCCCAACCCT
GCACTTTGGGGCCCGGGGGACTCAGGACCATTATCTTGTACGACATTTTTCCCAACGTTG
TCACAAAGTACCAATTTGTTTCAGGGCCCTAAATCGGAAACAAGCTCACTGGGGGCGACT
TGCC
Restriction Sites NotI-NotI |
ACCN NM_014657
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014657.1, NP_055472.1
RefSeq Size 3931 bp
RefSeq ORF 3270 bp
Locus ID 9675
UniProt ID O43156
Cytogenetics 20q11.23
Summary Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform.
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Documents
Resources
"NM_014657" in other vectors (7)
SKU Description Size Price
RC201622 TTI1 (Myc-DDK-tagged)-Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1) 10 ug
$948.00
RC201622L1 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), Myc-DDK-tagged 10 ug
$1,248.00
RC201622L2 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), mGFP tagged 10 ug
$1,248.00
RC201622L3 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), Myc-DDK-tagged 10 ug
$1,248.00
RC201622L4 Lenti ORF clone of Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1), mGFP tagged 10 ug
$1,248.00
RG201622 TTI1 (tGFP-tagged) - Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1) 10 ug
$789.00 $1,148.00
SC324266 TTI1 (untagged)-Human Tel2 interacting protein 1 homolog (S. pombe) (TTI1) 10 ug
$949.00

file-alt Citations

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