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MAT2A (NM_005911) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200926
MAT2A (Myc-DDK-tagged)-Human methionine adenosyltransferase II, alpha (MAT2A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAT2A
Synonyms MATA2; MATII; SAMS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200926 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGGACAGCTCAACGGCTTCCACGAGGCGTTCATCGAGGAGGGCACATTCCTTTTCACCTCAGAGT
CGGTCGGGGAAGGCCACCCAGATAAGATTTGTGACCAAATCAGTGATGCTGTCCTTGATGCCCACCTTCA
GCAGGATCCTGATGCCAAAGTAGCTTGTGAAACTGTTGCTAAAACTGGAATGATCCTTCTTGCTGGGGAA
ATTACATCCAGAGCTGCTGTTGACTACCAGAAAGTGGTTCGTGAAGCTGTTAAACACATTGGATATGATG
ATTCTTCCAAAGGTTTTGACTACAAGACTTGTAACGTGCTGGTAGCCTTGGAGCAACAGTCACCAGATAT
TGCTCAAGGTGTTCATCTTGACAGAAATGAAGAAGACATTGGTGCTGGAGACCAGGGCTTAATGTTTGGC
TATGCCACTGATGAAACTGAGGAGTGTATGCCTTTAACCATTGTCTTGGCACACAAGCTAAATGCCAAAC
TGGCAGAACTACGCCGTAATGGCACTTTGCCTTGGTTACGCCCTGATTCTAAAACTCAAGTTACTGTGCA
GTATATGCAGGATCGAGGTGCTGTGCTTCCCATCAGAGTCCACACAATTGTTATATCTGTTCAGCATGAT
GAAGAGGTTTGTCTTGATGAAATGAGGGATGCCCTAAAGGAGAAAGTCATCAAAGCAGTTGTGCCTGCGA
AATACCTTGATGAGGATACAATCTACCACCTACAGCCAAGTGGCAGATTTGTTATTGGTGGGCCTCAGGG
TGATGCTGGTTTGACTGGACGCAAAATCATTGTGGACACTTATGGCGGTTGGGGTGCTCATGGAGGAGGT
GCCTTTTCAGGAAAGGATTATACCAAGGTCGACCGTTCAGCTGCTTATGCTGCTCGTTGGGTGGCAAAAT
CCCTTGTTAAAGGAGGTCTGTGCCGGAGGGTTCTTGTTCAGGTCTCTTATGCTATTGGAGTTTCTCATCC
ATTATCTATCTCCATTTTCCATTATGGTACCTCTCAGAAGAGTGAGAGAGAGCTATTAGAGATTGTGAAG
AAGAATTTCGATCTCCGCCCTGGGGTCATTGTCAGGGATCTGGATCTGAAGAAGCCAATTTATCAGAGGA
CTGCAGCCTATGGCCACTTTGGTAGGGACAGCTTCCCATGGGAAGTGCCCAAAAAGCTTAAATAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200926 protein sequence
Red=Cloning site Green=Tags(s)

MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGE
ITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFG
YATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHD
EEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGG
AFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVK
KNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005911
ORF Size 1185 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005911.6
RefSeq Size 3022 bp
RefSeq ORF 1188 bp
Locus ID 4144
UniProt ID P31153, A0A140VJP5
Cytogenetics 2p11.2
Domains S-AdoMet_synt
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
MW 43.7 kDa
Summary The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. provided by RefSeq, Jun 2011
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Documents
Resources
"NM_005911" in other vectors (7)
SKU Description Size Price
RC200926L1 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), Myc-DDK-tagged 10 ug
$986.00
RC200926L2 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), mGFP tagged 10 ug
$986.00
RC200926L3 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), Myc-DDK-tagged 10 ug
$986.00
RC200926L4 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), mGFP tagged 10 ug
$986.00
RG200926 MAT2A (tGFP-tagged) - Human methionine adenosyltransferase II, alpha (MAT2A) 10 ug
$886.00
SC116444 MAT2A (untagged)-Human methionine adenosyltransferase II, alpha (MAT2A) 10 ug
$686.00
SC324396 MAT2A (untagged)-Human methionine adenosyltransferase II, alpha (MAT2A) 10 ug
$686.00

Plasmid Map for RC200926

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200926
Circular map for RC200926

file-alt Citations

Role of Methionine Adenosyltransferase α2 and β Phosphorylation and Stabilization in Human Hepatic Stellate Cell Trans-Differentiation
Ramani, Donoyan, Tomasi et al
J. Cell. Physiol. (2014)
Cited by 32
AI Summary: In this study, OriGene’s MAT2A-DDK (human MAT2A-DDK-tag in pCMV6-entry) and MEF2A antibodies were used to investigate the phosphorylation and stabilization of MAT proteins during hepatic stellate cell trans-differentiation, helping to elucidate their role in liver fibrogenesis.
Snippet: MAT2A-DDK (human MAT2A-DDK-tag in pCMV6-entry) (Origene);MAT2B-HA (MAT2B-3X-HA-tag human in pReceiver-M06) (Genecopoeia, Rockville, MD). ... (Novus), MEF2A (Origene), Anti-Phosphorylated Proteins (Pan) antibody (Invitrogen), p-ERK1/2 and B- ... Cite SaveSaving
Showing 1-1 of 1 papers.
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