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MAT2A (NM_005911) Human Untagged Clone

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SKU
SC116444
MAT2A (untagged)-Human methionine adenosyltransferase II, alpha (MAT2A)
  $686.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MAT2A
Synonyms MATA2; MATII; SAMS2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC116444 sequence for NM_005911 edited (data generated by NextGen Sequencing)
ATGAACGGACAGCTCAACGGCTTCCACGAGGCGTTCATCGAGGAGGGCACATTCCTTTTC
ACCTCAGAGTCGGTCGGGGAAGGCCACCCAGATAAGATTTGTGACCAAATCAGTGATGCT
GTCCTTGATGCCCACCTTCAGCAGGATCCTGATGCCAAAGTAGCTTGTGAAACTGTTGCT
AAAACTGGAATGATCCTTCTTGCTGGGGAAATTACATCCAGAGCTGCTGTTGACTACCAG
AAAGTGGTTCGTGAAGCTGTTAAACACATTGGATATGATGATTCTTCCAAAGGTTTTGAC
TACAAGACTTGTAACGTGCTGGTAGCCTTGGAGCAACAGTCACCAGATATTGCTCAAGGT
GTTCATCTTGACAGAAATGAAGAAGACATTGGTGCTGGAGACCAGGGCTTAATGTTTGGC
TATGCCACTGATGAAACTGAGGAGTGTATGCCTTTAACCATTGTCTTGGCACACAAGCTA
AATGCCAAACTGGCAGAACTACGCCGTAATGGCACTTTGCCTTGGTTACGCCCTGATTCT
AAAACTCAAGTTACTGTGCAGTATATGCAGGATCGAGGTGCTGTGCTTCCCATCAGAGTC
CACACAATTGTTATATCTGTTCAGCATGATGAAGAGGTTTGTCTTGATGAAATGAGGGAT
GCCCTAAAGGAGAAAGTCATCAAAGCAGTTGTGCCTGCGAAATACCTTGATGAGGATACA
ATCTACCACCTACAGCCAAGTGGCAGATTTGTTATTGGTGGGCCTCAGGGTGATGCTGGT
TTGACTGGACGCAAAATCATTGTGGACACTTATGGCGGTTGGGGTGCTCATGGAGGAGGT
GCCTTTTCAGGAAAGGATTATACCAAGGTCGACCGTTCAGCTGCTTATGCTGCTCGTTGG
GTGGCAAAATCCCTTGTTAAAGGAGGTCTGTGCCGGAGGGTTCTTGTTCAGGTCTCTTAT
GCTATTGGAGTTTCTCATCCATTATCTATCTCCATTTTCCATTATGGTACCTCTCAGAAG
AGTGAGAGAGAGCTATTAGAGATTGTGAAGAAGAATTTCGATCTCCGCCCTGGGGTCATT
GTCAGGGATCTGGATCTGAAGAAGCCAATTTATCAGAGGACTGCAGCCTATGGCCACTTT
GGTAGGGACAGCTTCCCATGGGAAGTGCCCAAAAAGCTTAAATATTGA

Clone variation with respect to NM_005911.5
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005911 unedited
TTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCTACGAGTAGAACGCT
GTCCGCAGCTTGCGCATTTCGCAGCCGCTGCCGCCTCGCCGCTGCTCCTTCGTAAGGCCA
CTTCCGCACACCGACACCAACATGAACGGACAGCTCAACGGCTTCCACGAGGCGTTCATC
GAGGAGGGCACATTCCTTTTCACCTCAGAGTCGGTCGGGGAAGGCCACCCAGATAAGATT
TGTGACCAAATCAGTGATGCTGTCCTTGATGCCCACCTTCAGCAGGATCCTGATGCCAAA
GTAGCTTGTGAAACTGTTGCTAAAACTGGAATGATCCTTCTTGCTGGGGAAATTACATCC
AGAGCTGCTGTTGACTACCAGAAAGTGGTTCGTGAAGCTGTTAAACACATTGGATATGAT
GATTCTTCCAAAGGTTTTGACTACAAGACTTGTAACGTGCTGGTAGCCTTGGAGCAACAG
TCACCAGATATTGCTCAAGGTGTTCATCTTGACAGAAATGAAGAAGACATTGGTGCTGGA
GACCAGGGCTTAATGTTTGGCTATGCCACTGATGAAACTGAGGAGTGTATGCCTTTAACC
ATTGTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGGCACTTTG
CCTTGGTTACGCCCTGATTCTAAACTCAATTACTGTGCAGATATGCAGGATCGAGGTGCT
GTGCTTCCATCAGAGTCACACAATTGTATATCTGTTAGCATGTAAAGAAGGTTTGTCTGA
TGAATGAGGGGATGCCCTAAGGGAGAAGTCTCAAAGCGNGTGGTCCTGCGGAATACCTGA
TGAGATACATNCTACACCTACAGNCAGTGGNANATTGTTTATGGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005911 unedited
ATGCACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGTAGAACCTAGC
ATTTTATTTAGATCTTCATTAAACTGTTGGAATTGAGAACCAGACATACGTAATAAACCT
CCAAAAATAGATCCTGAAAGGCACTTTCTGCTTAGGGCAAGCAGTCATGGAATAAGCATG
TAAACAAGCTGGCTTCTCTGTACCACACCAGCCAAGTCAGCTTTCTCCATGGCCAGCCGC
ACCAGCTCTGCCCTCCCTTCTGTTAACACCAGCCAGACCCCCTGTAGGTCTAACCCAAGG
TTTTTCTGTAGGACACCTTGGCCTACCTGGGAATGCTGGAAACATGTTGTTAAAGGAATC
ATGGTGTTGAGAGAAAAAAAAAAAATCTTTTAAAAGCTGCCATCTGAGGTGATGGCTTCT
CTGTACTTACGCCATACCCCAGAATACAATAAATAAGCAATTAGAAAACGTTCAAGTATG
AAGGGATTTCCTCCTCCCCGCCAAAAGCACTGCTCTCTGAAGGAAGCTGGTTTCTCTGTA
CCTACACCAGCTGTTCAAAAAGCTCATTGGACCTGGTTTTGAAAATAAAACAAAGTTAAA
ACCCTGGGAGGAGTTATTGTGCAGTGTGGAGTACTCAGGCTTTCTTATAAAGAAAAAAAA
AGTTATCTGGTACCAAAGTGTGCACCCTACAGACCCCTCAGTACTGCCCTGTGACTTCTC
TGTATGACATCACAAGGCTGCCAAGTGCCTGTTTTTCTAGACTAAGAGTTGNTGAGGTTT
GCTAGTGCTGAAACATGCATAGAATTGGTTACTAACTAAAACCTTATACGTACGTCTCCA
AAGAAGCTGAAAGTGTAGAGATTTGATTTGCGCTGTTAGAGTGGCATTACGACATATCCC
CAGGGAGAGGTTAATGGTACTGCCTCTTTGAGCCAGGCTGAGCTCCACTGGAACTTGGT
Restriction Sites NotI-NotI |
ACCN NM_005911
Insert Size 2820 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005911.4, NP_005902.1
RefSeq Size 2835 bp
RefSeq ORF 1188 bp
Locus ID 4144
UniProt ID P31153
Cytogenetics 2p11.2
Domains S-AdoMet_synt
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
Summary The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. provided by RefSeq, Jun 2011
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Documents
Resources
"NM_005911" in other vectors (7)
SKU Description Size Price
RC200926 MAT2A (Myc-DDK-tagged)-Human methionine adenosyltransferase II, alpha (MAT2A) 10 ug
$686.00
RC200926L1 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), Myc-DDK-tagged 10 ug
$986.00
RC200926L2 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), mGFP tagged 10 ug
$986.00
RC200926L3 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), Myc-DDK-tagged 10 ug
$986.00
RC200926L4 Lenti ORF clone of Human methionine adenosyltransferase II, alpha (MAT2A), mGFP tagged 10 ug
$986.00
RG200926 MAT2A (tGFP-tagged) - Human methionine adenosyltransferase II, alpha (MAT2A) 10 ug
$886.00
SC324396 MAT2A (untagged)-Human methionine adenosyltransferase II, alpha (MAT2A) 10 ug
$686.00

file-alt Citations

Methionine adenosyltransferase 2A/2B and methylation: gene sequence variation and functional genomics
Nordgren, Peng, Pelleymounter et al
Drug Metab. Dispos. (2011) 39 (11), 2135-47 DOI: 10.1124/dmd.111.040857
Cited by 27
AI Summary: OriGene products contributed to this study by providing the MAT2A (SC116444), MAT2B V1 (SC110397), and MAT2B V2 (SC110398) clones, which were used as templates to amplify open reading frames for functional genomic analyses.
Snippet: The open reading frames (ORFs) of MAT2A, MAT2B V1, and MAT2B V2 were amplified from Origene clones SC116444, SC110397, and SC110398 (Origene, Rockville, MD), respectively, and each amplicon was subcloned into pcDNA3.1D/V5-His-TOPO (Invitrogen, Carlsbad
Showing 1-1 of 1 papers.
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