Stat3 (NM_213659) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR227265
Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1
$1,057.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Stat3 |
| Synonyms | 1110034C02Rik; A; Aprf; AW109958 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_213659 |
| ORF Size | 2310 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_213659.3 |
| RefSeq Size | 4487 bp |
| RefSeq ORF | 2313 bp |
| Locus ID | 20848 |
| UniProt ID | P42227 |
| Cytogenetics | 11 63.82 cM |
| MW | 88.5 kDa |
| Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_213659" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC221487 | Stat3 (untagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1, (10ug) | 10 ug |
$1,059.00
|
|
| MG227265 | Stat3 (tGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3) transcript variant 1, (10ug) | 10 ug |
$1,257.00
|
|
| MR227265L1 | Lenti ORF clone of Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
| MR227265L2 | Lenti ORF clone of Stat3 (mGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
| MR227265L3 | Lenti ORF clone of Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
| MR227265L4 | Lenti ORF clone of Stat3 (mGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
Citations
linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses
Chen, He, Zhu et al
Cell Rep (2021) 34 (1), 108584 DOI: 10.1016/j.celrep.2020.108584
Chen, He, Zhu et al
Cell Rep (2021) 34 (1), 108584 DOI: 10.1016/j.celrep.2020.108584
Cited by 18
AI Summary: OriGene products, including Rela (Myc-DDK-tagged)-pCMV6 (Cat# MR227671), Stat3 (Myc-DDK-tagged)-pCMV6 (Cat# MR227265), and pCMV6-Entry (Cat# PS100001), were used as expression plasmids to investigate the molecular mechanisms underlying linc-AAM-mediated gene regulation in macrophage activation and immune responses.
Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Malt1 signaling in neuroinflammation
Cho, Xu, Parthasarathy et al
Nat Commun (2019) 10 (1), 701 DOI: 10.1038/s41467-019-08605-3
Cho, Xu, Parthasarathy et al
Nat Commun (2019) 10 (1), 701 DOI: 10.1038/s41467-019-08605-3
AI Summary: OriGene’s mouse Hectd3 clone (Cat#MC206119) was used as a template for PCR amplification of Hectd3, enabling the study of Hectd3’s role in Th17 cell differentiation and neuroinflammation.
Snippet: Taq Version 2.0) according to manufacturer’s protocol (Cat#RR003A, Takara Bio USA, CA, USA) with the Hectd3 primers listed in Supplementary Table 4, and template mouse Hectd3 clone (Cat#MC206119, Origene, MD, USA). Hectd3 PCR product containing 5′ BglII site and 3′ XhoI site were subcloned into pGEM-T vector following manufacturer’s protocol (Cat#A3600, Promega, WI, USA). Subcloned Hectd
Inflammation-induced Id2 promotes plasticity in regulatory T cells
Hwang, Sharma, Verma et al
Nat Commun (2018) 9 (1), 4736 DOI: 10.1038/s41467-018-07254-2
Hwang, Sharma, Verma et al
Nat Commun (2018) 9 (1), 4736 DOI: 10.1038/s41467-018-07254-2
AI Summary: OriGene products—STAT3 (MR227265), IRF4 (MR226642), BATF (MR222114), E2A (MG209745), and Id2 (MR200792) expression plasmids—were used in co-transfection experiments to investigate the regulatory mechanisms controlling Id2 and Foxp3 promoter activity in T cell plasticity.
Snippet: (Lonza) according to the manufacturer’s protocol and plated in 12 well plate. 0.5 μg of the _Id2_ promoter-luciferase constructs or pGL4.17 empty vector was co-transfected with STAT3 (MR227265;Origene), IRF4 (MR226642;Origene) and BATF (MR222114;Origene) expression plasmids (HEK-293 T) and 1 μg of the _Foxp3_ promoter-luciferase construct was co-transfected with E2A (MG209745;Origene);HEK-293 T cells or Jurkat cells were transfected using FuGENE HD (Promega) or NucleofectorTM (Lonza) according to the manufacturer’s protocol and plated in 12 well plate. 0.5 μg of the Id2 promoter-luciferase constructs or pGL4.17 empty vector was co-transfected with STAT3 (MR227265;Origene) expression plasmids (HEK-293 T) and 1 μg of the Foxp3 promoter-luciferase construct was co-transfected with E2A (MG209745;Origene) and Id2 (MR200792;Origene) expression plasmids (Jurkat). …
Showing 1-3 of 3 papers.
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