Stat3 (NM_213659) Mouse Untagged Clone
SKU
MC221487
Stat3 (untagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1, (10ug)
$1,059.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Stat3 |
| Synonyms | 1110034C02Rik; A; Aprf; AW109958 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_213659 |
| Insert Size | 2313 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC003806, AAH03806 |
| RefSeq Size | 2964 bp |
| RefSeq ORF | 2313 bp |
| Locus ID | 20848 |
| UniProt ID | P42227 |
| Cytogenetics | 11 63.82 cM |
| Summary | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Sep 2015 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
"NM_213659" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG227265 | Stat3 (tGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3) transcript variant 1, (10ug) | 10 ug |
$1,257.00
|
|
| MR227265 | Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,057.00
|
|
| MR227265L1 | Lenti ORF clone of Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
| MR227265L2 | Lenti ORF clone of Stat3 (mGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
| MR227265L3 | Lenti ORF clone of Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
| MR227265L4 | Lenti ORF clone of Stat3 (mGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 | 10 ug |
$1,357.00
|
|
Citations
Hypothalamic leptin action is mediated by histone deacetylase 5
Kabra, Pfuhlmann, García-Cáceres et al
Nat Commun (2016) 7, 10782 DOI: 10.1038/ncomms10782
Kabra, Pfuhlmann, García-Cáceres et al
Nat Commun (2016) 7, 10782 DOI: 10.1038/ncomms10782
Cited by 91
AI Summary: In this study, the OriGene mouse STAT3 overexpression plasmid was used to transfect cells, enabling the investigation of STAT3’s role in hypothalamic leptin signaling and its regulation by HDAC5.
Snippet: Proteins were transferred on nitrocellulose membranes using a Trans Blot Turbo transfer apparatus (Biorad, Hercules, CA, USA) and stained with either anti-HDAC5 (mouse monoclonal, 1:2,000, Cat # sc-133106, Santacruz, CA, USA), or anti-POMC (goat polyclonal, 1:2,000, Cat # ab32893, abcam) or anti-Ac-STAT3K685 (rabbit polyclonal, 1:500, Cat #2523,), anti-p-STAT3T705 (rabbit polyclonal, 1:500, Cat #9145), anti-STAT3 (mouse monoclonal, 1:2,000, Cat #9139), and anti-ACTIN (1:10,000, rabbit polyclonal, Cat #4970) from Cell Signaling Technology. …Adenoviral delivery of HDAC5 was conducted in CLU177 cells that were first transfected with overexpresser mouse STAT3 plasmid (OriGene, Rockville, MD, USA) using lipofectamine. After 24 h of transfection, cells were incubated with recombinant replication-deficient adenovirus expressing HDAC5–GFP or control adenovirus. …
A novel role of microRNA146b in promoting mammary alveolar progenitor cell maintenance
Elsarraj, Hong, Valdez et al
J. Cell. Sci. (2013) 126 (Pt 11), 2446-58 DOI: 10.1242/jcs.119214
Elsarraj, Hong, Valdez et al
J. Cell. Sci. (2013) 126 (Pt 11), 2446-58 DOI: 10.1242/jcs.119214
Cited by 20
AI Summary: In this study, OriGene’s STAT3 (Cat# TA313521) and β-casein (Cat# TA326524) antibodies were used for western blot analyses to assess protein expression changes following miR146b knockdown in mouse mammary epithelial cells, helping to elucidate the molecular mechanisms underlying progenitor cell maintenance.
Showing 1-2 of 2 papers.
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