Stat3 (NM_213659) Mouse Tagged Lenti ORF Clone

  • LentiORF®
SKU
MR227265L3
Lenti ORF clone of Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1
  $1,357.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Stat3
Synonyms 1110034C02Rik; A; Aprf; AW109958
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR227265).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_213659
ORF Size 2310 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_213659.2
RefSeq Size 4487 bp
RefSeq ORF 2313 bp
Locus ID 20848
UniProt ID P42227
Cytogenetics 11 63.82 cM
Summary The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Sep 2015
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"NM_213659" in other vectors (6)
SKU Description Size Price
MC221487 Stat3 (untagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1, (10ug) 10 ug
$1,059.00
MG227265 Stat3 (tGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3) transcript variant 1, (10ug) 10 ug
$1,257.00
MR227265 Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 10 ug
$1,057.00
MR227265L1 Lenti ORF clone of Stat3 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 10 ug
$1,357.00
MR227265L2 Lenti ORF clone of Stat3 (mGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 10 ug
$1,357.00
MR227265L4 Lenti ORF clone of Stat3 (mGFP-tagged) - Mouse signal transducer and activator of transcription 3 (Stat3), transcript variant 1 10 ug
$1,357.00

Plasmid Map for MR227265L3

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Cloning scheme for MR227265L3

Circular map for MR227265L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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