Adrb3 (NM_013462) Mouse Tagged ORF Clone
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Adrb3 |
| Synonyms | Adrb-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013462 |
| ORF Size | 1200 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013462.3, NP_038490.2 |
| RefSeq Size | 2795 bp |
| RefSeq ORF | 1203 bp |
| Locus ID | 11556 |
| UniProt ID | P25962 |
| Cytogenetics | 8 15.94 cM |
| MW | 43.5 kDa |
| Summary | Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013462" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208113 | Adrb3 (untagged) - Mouse adrenergic receptor, beta 3 (Adrb3), (10ug) | 10 ug |
$457.00
|
|
| MG225687 | Adrb3 (tGFP-tagged) - Mouse adrenergic receptor beta 3 (Adrb3), (10ug) | 10 ug |
$489.00
$657.00
|
|
| MR225687L3 | Lenti ORF clone of Adrb3 (Myc-DDK-tagged) - Mouse adrenergic receptor, beta 3 (Adrb3) | 10 ug |
$757.00
|
|
| MR225687L4 | Lenti ORF clone of Adrb3 (mGFP-tagged) - Mouse adrenergic receptor, beta 3 (Adrb3) | 10 ug |
$757.00
|
|
Citations
Adaptive thermogenesis in brown adipose tissue involves activation of pannexin-1 channels
Senthivinayagam, Serbulea, Upchurch et al
Mol Metab (2020) 44, 101130 DOI: 10.1016/j.molmet.2020.101130
Senthivinayagam, Serbulea, Upchurch et al
Mol Metab (2020) 44, 101130 DOI: 10.1016/j.molmet.2020.101130
Cited by 36
AI Summary: In this study, the OriGene cDNA clone for mouse myc-DDK-adrenergic receptor beta-3 (ADRB3, MR225687) was used to generate expression constructs for investigating the role of β3-adrenergic signaling in brown adipocyte thermogenesis.
Snippet: … cDNA clone for mouse myc-DDK-adrenergic receptor beta-3 (ADRB3) (MR225687) was purchased from Origene (Rockville, MD). To generate mCherry-tagged-mADRB3, mADRB3 was polymerase chain reaction (PCR) amplified with SpeI-Not restriction sites from the Origene clone without myc-DDK and cloned into;Panx1 mutant lacking putative Gβγ binding site (Panx1-∆βγ) was made with the following primers (Forward-CAAATCTTTGAACTCCACGTCCTCTCCCTTGGTCT;ReverseAGACCAAGGGA GAGGACGTGG AGTTCAAAGATTTG) using Quick-change site directed mutagenesis kit (Agilent Technologies, Santa Clara CA). cDNA clone for mouse myc-DDKadrenergic receptor beta-3 (ADRB3) (MR225687) was purchased from Origene (Rockville, MD). To generate mCherry-tagged-mADRB3, mADRB3 was PCR amplified with SpeI-Not restriction sites from the Origene clone without myc-DDK and cloned into pcDNA3.1 E5
Adipocyte β-arrestin-2 is essential for maintaining whole body glucose and energy homeostasis
Pydi, Jain, Tung et al
Nat Commun (2019) 10 (1), 2936 DOI: 10.1038/s41467-019-11003-4
Pydi, Jain, Tung et al
Nat Commun (2019) 10 (1), 2936 DOI: 10.1038/s41467-019-11003-4
AI Summary: OriGene’s mouse β3-adrenergic receptor expression construct (pCMV6 vector) was used to study β3-AR signaling in cells, enabling the authors to elucidate the regulatory role of β-arrestin-2 in adipocyte function and metabolism.
Snippet: β2-AR (vector backbone: pcDNA 3.1) was kindly provided by Dr. Vsevolod Gurevich (Vanderbilt Univesity). The expression construct encoding the mouse β3-AR (vector backbone: pCMV6) was purchased from OriGene Technologies Inc. Six hour after transfections, 1 × 105 cells were plated in collagen-coated 24-well plates. Cells were allowed to grow for 48 h and then used for experiments. BODY COM
Showing 1-2 of 2 papers.
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