Adrb3 (NM_013462) Mouse Tagged ORF Clone
SKU
MR225687
Adrb3 (Myc-DDK-tagged) - Mouse adrenergic receptor, beta 3 (Adrb3)
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Target Symbol | Adrb3 |
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Synonyms | Adrb-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>MR225687 representing NM_013462
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCGTGGCCTCACAGAAACGGCTCTCTGGCTTTGTGGTCGGACGCCCCTACCCTGGACCCCAGTG CAGCCAACACCAGTGGGTTGCCAGGAGTACCATGGGCAGCGGCATTGGCTGGGGCATTGCTGGCGCTGGC CACGGTGGGAGGCAACCTGCTGGTAATCATAGCCATCGCCCGCACGCCGAGACTACAGACCATAACCAAC GTGTTCGTGACTTCACTGGCCGCAGCTGACTTGGTAGTGGGACTCCTCGTAATGCCACCAGGGGCCACAT TGGCGCTGACTGGCCATTGGCCCTTGGGCGAAACTGGTTGCGAACTGTGGACGTCAGTGGACGTGCTCTG TGTAACTGCTAGCATCGAGACCTTGTGCGCCCTGGCTGTGGACCGCTACCTAGCTGTCACCAACCCTTTG CGTTACGGCACGCTGGTTACCAAGCGCCGCGCCCGCGCGGCAGTTGTCCTGGTGTGGATCGTGTCCGCTG CCGTGTCCTTTGCGCCCATCATGAGCCAGTGGTGGCGTGTAGGGGCAGATGCCGAGGCACAGGAATGCCA CTCCAATCCGCGCTGCTGTTCCTTTGCCTCCAACATGCCCTATGCGCTGCTCTCCTCCTCCGTCTCCTTC TACCTTCCCCTCCTTGTGATGCTCTTCGTCTATGCTCGAGTGTTCGTTGTGGCTAAGCGCCAACGGCATT TGCTGCGCCGGGAACTGGGCCGCTTCTCGCCCGAGGAGTCTCCGCCGTCTCCGTCGCGCTCTCCGTCCCC TGCCACAGGCGGGACACCCGCGGCACCGGATGGAGTGCCCCCCTGCGGCCGGCGGCCTGCGCGCCTCCTG CCACTCCGGGAACACCGCGCCCTGCGCACCTTAGGTCTCATTATGGGCATCTTCTCTCTGTGCTGGCTGC CCTTCTTCCTGGCCAACGTGCTGCGCGCACTCGCGGGGCCCTCTCTAGTTCCCAGCGGAGTTTTCATCGC CCTGAACTGGCTGGGCTATGCCAACTCCGCCTTCAACCCGGTCATCTACTGCCGCAGCCCGGACTTTCGC GACGCCTTCCGTCGTCTTCTGTGTAGCTACGGTGGCCGTGGACCGGAGGAGCCACGCGCAGTCACCTTCC CAGCCAGCCCTGTTGAAGCCAGGCAGAGTCCACCGCTCAACAGGTTTGATGGCTATGAAGGTGCGCGTCC GTTTCCCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR225687 representing NM_013462
Red=Cloning site Green=Tags(s) MAPWPHRNGSLALWSDAPTLDPSAANTSGLPGVPWAAALAGALLALATVGGNLLVIIAIARTPRLQTITN VFVTSLAAADLVVGLLVMPPGATLALTGHWPLGETGCELWTSVDVLCVTASIETLCALAVDRYLAVTNPL RYGTLVTKRRARAAVVLVWIVSAAVSFAPIMSQWWRVGADAEAQECHSNPRCCSFASNMPYALLSSSVSF YLPLLVMLFVYARVFVVAKRQRHLLRRELGRFSPEESPPSPSRSPSPATGGTPAAPDGVPPCGRRPARLL PLREHRALRTLGLIMGIFSLCWLPFFLANVLRALAGPSLVPSGVFIALNWLGYANSAFNPVIYCRSPDFR DAFRRLLCSYGGRGPEEPRAVTFPASPVEARQSPPLNRFDGYEGARPFPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013462 |
ORF Size | 1200 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013462.3, NP_038490.2 |
RefSeq Size | 2795 bp |
RefSeq ORF | 1203 bp |
Locus ID | 11556 |
UniProt ID | P25962 |
Cytogenetics | 8 15.94 cM |
MW | 43.5 kDa |
Summary | Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.[UniProtKB/Swiss-Prot Function] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
MC208113 | Adrb3 (untagged) - Mouse adrenergic receptor, beta 3 (Adrb3), (10ug) | 10 ug |
$457.00
|
|
MG225687 | Adrb3 (tGFP-tagged) - Mouse adrenergic receptor beta 3 (Adrb3), (10ug) | 10 ug |
$489.00
MSRP
$657.00
MSRP
$657.00
|
|
MR225687L3 | Lenti ORF clone of Adrb3 (Myc-DDK-tagged) - Mouse adrenergic receptor, beta 3 (Adrb3) | 10 ug |
$757.00
|
|
MR225687L4 | Lenti ORF clone of Adrb3 (mGFP-tagged) - Mouse adrenergic receptor, beta 3 (Adrb3) | 10 ug |
$757.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.