Ptch2 (NM_008958) Mouse Tagged ORF Clone
$589.00
$1,029.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ptch2 |
| Synonyms | ptc; ptc2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008958 |
| ORF Size | 3546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008958.3, NP_032984.1 |
| RefSeq Size | 3549 bp |
| RefSeq ORF | 3549 bp |
| Locus ID | 19207 |
| UniProt ID | O35595 |
| Cytogenetics | 4 53.41 cM |
| MW | 129 kDa |
| Summary | This gene encodes a member of the patched family of transmembrane receptor proteins. The encoded protein may be a functional receptor for the morphogen sonic hedgehog (Shh) and is reportedly involved in limb and skin development. Homozygous mutant mice for this gene exhibit hair loss and epidermal hyperplasia. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008958" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223858 | Ptch2 (untagged) - Mouse patched homolog 2 (Ptch2), (10ug) | 10 ug |
$1,133.00
|
|
| MG224361 | Ptch2 (tGFP-tagged) - Mouse patched homolog 2 (Ptch2), (10ug) | 10 ug |
$1,229.00
|
|
| MR224361L3 | Lenti ORF clone of Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2) | 10 ug |
$1,329.00
|
|
| MR224361L4 | Lenti ORF clone of Ptch2 (mGFP-tagged) - Mouse patched homolog 2 (Ptch2) | 10 ug |
$1,329.00
|
|
Citations
Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration
Kim, Lee, Seppala et al
Nat Commun (2020) 11 (1), 1994 DOI: 10.1038/s41467-020-15897-3
Kim, Lee, Seppala et al
Nat Commun (2020) 11 (1), 1994 DOI: 10.1038/s41467-020-15897-3
Cited by 29
AI Summary: OriGene’s pCMV6-mPtch2-MycDDK plasmid was used for overexpression of Ptch2 in NIH3T3 cells, enabling the authors to investigate the distinct roles of Ptch2 in Hedgehog signalling during murine primordial germ cell migration.
Snippet: …For overexpression in NIH3T3 cells, the vector pcDNA3 (Addgene), pcDNA3-mPtch1 and pCMV6-mPtch2-MycDDK (Origene) were transiently transfected using Polyfect. … For overexpression in NIH3T3 cells, the vector pcDNA3 (Addgene), pcDNA3-mPtch1 and pCMV6-mPtch2-MycDDK (Origene) were transiently transfected using Polyfect. …
Showing 1-1 of 1 papers.
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