Ptch2 (NM_008958) Mouse Tagged Lenti ORF Clone

  • LentiORF®
SKU
MR224361L3
Lenti ORF clone of Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2)
  $1,329.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptch2
Synonyms ptc; ptc2
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR224361).
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_008958
ORF Size 3546 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008958.2, NP_032984.1
RefSeq Size 3549 bp
RefSeq ORF 3549 bp
Locus ID 19207
UniProt ID O35595
Cytogenetics 4 53.41 cM
Summary This gene encodes a member of the patched family of transmembrane receptor proteins. The encoded protein may be a functional receptor for the morphogen sonic hedgehog (Shh) and is reportedly involved in limb and skin development. Homozygous mutant mice for this gene exhibit hair loss and epidermal hyperplasia. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_008958" in other vectors (4)
SKU Description Size Price
MC223858 Ptch2 (untagged) - Mouse patched homolog 2 (Ptch2), (10ug) 10 ug
$1,133.00
MG224361 Ptch2 (tGFP-tagged) - Mouse patched homolog 2 (Ptch2), (10ug) 10 ug
$1,229.00
MR224361 Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2) 10 ug
$589.00 $1,029.00
MR224361L4 Lenti ORF clone of Ptch2 (mGFP-tagged) - Mouse patched homolog 2 (Ptch2) 10 ug
$1,329.00

Plasmid Map for MR224361L3

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Cloning scheme for MR224361L3

Circular map for MR224361L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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