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Ptch2 (NM_008958) Mouse Untagged Clone

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SKU
MC223858
Ptch2 (untagged) - Mouse patched homolog 2 (Ptch2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,133.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ptch2
Synonyms ptc; ptc2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223858 representing NM_008958
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCGGCCACTGTCCCTTGGAGAGCTACCTCCCAGCTACACACCTCCAGCTCGGTCCTCCGCACCTC
ATATCCTAGCTGGGAGCCTGCAGGCTCCTCTCTGGCTTCGAGCTTACTTCCAAGGTCTACTCTTCTCCCT
GGGGTGCAGGATCCAGAAACACTGCGGCAAAGTGCTCTTCCTGGGACTGGTGGCCTTTGGGGCTCTGGCA
CTGGGTCTCCGAGTGGCTGTAATTGAGACAGACCTAGAACAGCTCTGGGTAGAAGTGGGCAGCAGGGTGA
GCCAGGAGCTGCACTACACCAAGGAGAAGCTGGGGGAAGAGGCTGCGTACACCTCCCAGATGTTGATTCA
GACTGCGCACCAGGAAGGGGGAAACGTCCTCACCCCCGAGGCACTTGACTTGCACCTCCAGGCGGCGCTC
ACTGCCAGTAAAGTCCAAGTATCACTCTATGGGAAATCCTGGGATTTGAACAAGATCTGCTACAAATCAG
GGGTTCCCCTTATTGAAAATGGGATGATCGAGCGGATGATTGAGAAGCTGTTTCCCTGTGTGATCCTCAC
CCCGCTTGACTGCTTCTGGGAAGGAGCCAAACTCCAAGGGGGCTCTGCCTACTTGCCGGGCCGCCCTGAT
ATCCAGTGGACCAACCTGGACCCTCAGCAGCTGCTCGAGGAGCTGGGCCCCTTTGCCTCTCTGGAGGGCT
TCCGGGAACTGCTAGATAAGGCACAGGTGGGCCAGGCCTATGTGGGGCGACCCTGTCTGGACCCTGATGA
CCCCCACTGTCCACCTAGTGCTCCCAACCGGCACAGCAGGCAGGCTCCCAATGTGGCTCAGGAGCTGAGT
GGGGGCTGCCATGGCTTCTCCCACAAGTTCATGCACTGGCAGGAGGAACTGCTACTAGGGGGCACAGCCA
GAGATCTCCAAGGACAGCTGCTGAGGGCAGAGGCCTTGCAGAGCACCTTCCTGCTCATGAGTCCCCGTCA
GCTGTACGAGCACTTCCGGGGCGACTACCAGACACATGACATCGGCTGGAGCGAGGAGCAGGCCAGCATG
GTGCTGCAGGCCTGGCAGAGGCGCTTTGTGCAGCTAGCCCAGGAGGCTCTGCCGGCCAACGCGTCCCAGC
AGATCCATGCCTTCTCCTCCACCACCCTGGATGACATCCTGCGCGCGTTCTCTGAAGTCAGCACCACCCG
TGTGGTAGGAGGCTATCTGCTTATGCTGGCCTATGCCTGCGTAACAATGCTGCGGTGGGACTGCGCCCAG
TCCCAGGGTGCCGTAGGTCTTGCTGGGGTGTTGCTGGTGGCCCTGGCGGTGGCCTCAGGTCTTGGGCTTT
GTGCCCTACTTGGCATCACTTTCAATGCTGCCACTACACAGGTGCTGCCCTTCTTGGCTCTGGGCATTGG
CGTGGATGACATCTTCCTGCTGGCGCATGCCTTCACAAAAGCCCCACCTGACACCCCTCTCCCAGAGCGT
ATGGGTGAGTGTTTGAGGAGCACGGGCACCAGCGTGGCACTCACATCCGTCAACAACATGGTCGCCTTTT
TCATGGCTGCCCTGGTTCCCATTCCTGCGCTGCGGGCCTTCTCCCTGCAGGCAGCCATAGTGGTCGGCTG
CAACTTTGCAGCGGTAATGCTCGTCTTCCCTGCCATCCTCAGCCTCGACCTGCGCCGGCGCCACCGCCAA
CGCCTGGACGTTCTCTGCTGCTTTTCTAGCCCCTGCTCCGCTCAGGTCATTCAGATGCTGCCCCAGGAGC
TAGGGGACAGAGCAGTACCAGTGGGCATTGCCCACCTGACTGCCACCGTGCAAGCCTTCACCCACTGCGA
AGCCAGCAGCCAGCATGTAGTCACCATTTTGCCTCCTCAAGCCCACCTGCTGTCTCCAGCTTCTGACCCA
CTGGGCTCCGAACTCTATAGCCCTGGAGGGTCTACACGGGACCTTCTCAGTCAGGAGGAGGGGACAGGGC
CACAGGCGGCCTGCAGGCCCCTGCTCTGTGCCCACTGGACTCTCGCCCATTTTGCCCGCTATCAGTTTGC
ACCTTTACTGCTCCAGACACGAGCCAAGGCCCTGGTGTTGCTGTTCTTTGGGGCTCTTTTGGGCCTGAGC
CTCTATGGAGCCACCTTGGTACAAGATGGGCTGGCCCTGACAGATGTGGTCCCTAGGGGCACCAAGGAAC
ATGCTTTCCTGAGCGCCCAGCTCAGGTACTTCTCCCTGTACGAGGTGGCTCTAGTGACACAGGGTGGCTT
TGACTACGCCCACTCCCAACGCGCCCTCTTTGATCTGCACCAGCGCTTCAGCTCCCTCAAGGCTGTGCTG
CCCCCACCTGCCACCCAGGCACCCCGCACCTGGCTTCACTACTACCGCAGCTGGCTACAGGGTATCCAGG
CTGCATTTGACCAAGACTGGGCTTCTGGGCGCATCACCTGCCACTCTTACCGCAACGGCTCAGAAGATGG
CGCCCTGGCCTACAAGCTGCTCATCCAAACCGGGAATGCCCAGGAGCCTCTGGATTTCAGCCAGCTGACC
ACAAGGAAACTGGTGGACAAGGAGGGACTCATTCCCCCAGAGCTCTTCTACATGGGGCTAACCGTGTGGG
TGAGCAGCGACCCCCTGGGCCTCGCAGCCTCTCAGGCCAACTTCTACCCCCCACCTCCAGAGTGGCTTCA
CGACAAATATGATACCACCGGGGAGAACCTTCGCATCCCGGCAGCCCAGCCCTTGGAGTTTGCCCAGTTC
CCCTTCCTGCTGCATGGACTCCAGAAGACTGCAGACTTTGTAGAAGCCATCGAAGGGGCCAGGGCGGCAT
GCACAGAGGCAGGCCAGGCAGGGGTGCATGCCTACCCCAGTGGCTCCCCCTTCCTCTTCTGGGAGCAGTA
TCTGGGTCTTCGGCGCTGCTTCCTGCTGGCAGTCTGCATCTTGCTGGTGTGCACCTTCCTCGTCTGTGCC
CTGCTTCTACTCAGCCCGTGGACAGCTGGCCTCATAGTGCTGGTCCTGGCAATGATGACTGTGGAGCTCT
TTGGTATCATGGGATTCCTGGGCATCAAACTGAGTGCCATCCCCGTGGTAATCCTCGTGGCCTCTATAGG
CATTGGTGTTGAATTCACAGTTCACGTGGCTCTGGGCTTCCTGACCAGCCATGGTAGCCGGAACCTGCGG
GCTGCTAGCGCTCTGGAACAGACCTTTGCCCCTGTGACTGATGGAGCTGTCTCCACCTTGCTGGGTCTGC
TCATGCTTGCTGGTTCTAACTTTGACTTCATCATAAGGTATTTCTTTGTGGTGCTGACGGTGCTGACACT
CTTGGGCCTGCTCCATGGACTCCTGCTGCTGCCTGTGCTGCTCTCTATCCTAGGCCCCCCGCCACAGGTG
GTGCAGGTATACAAGGAGAGTCCACAGACCCTAAACTCCGCTGCTCCACAGAGAGGTGGGCTCAGGTGGG
ACAGGCCCCCCACCCTGCCCCAGAGTTTTGCCAGAGTGACTACCTCCATGACTGTGGCCCTCCACCCACC
ACCTCTGCCTGGAGCATACGTCCACCCAGCCTCCGAGGAGCCCACATAG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_008958
Insert Size 3549 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008958.3, NP_032984.1
RefSeq Size 5119 bp
RefSeq ORF 3549 bp
Locus ID 19207
UniProt ID O35595
Cytogenetics 4 53.41 cM
Summary This gene encodes a member of the patched family of transmembrane receptor proteins. The encoded protein may be a functional receptor for the morphogen sonic hedgehog (Shh) and is reportedly involved in limb and skin development. Homozygous mutant mice for this gene exhibit hair loss and epidermal hyperplasia. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2015
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_008958" in other vectors (4)
SKU Description Size Price
MG224361 Ptch2 (tGFP-tagged) - Mouse patched homolog 2 (Ptch2), (10ug) 10 ug
$1,229.00
MR224361 Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2) 10 ug
$589.00 $1,029.00
MR224361L3 Lenti ORF clone of Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2) 10 ug
$1,329.00
MR224361L4 Lenti ORF clone of Ptch2 (mGFP-tagged) - Mouse patched homolog 2 (Ptch2) 10 ug
$1,329.00

file-alt Citations

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