Hsd17b1 (NM_010475) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR222239
Hsd17b1 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1)
$289.00
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hsd17b1 |
| Synonyms | 17beta-HSD; 17HSDB1; E2DH; Hsd17ba |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_010475 |
| ORF Size | 1032 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_010475.2 |
| RefSeq Size | 1317 bp |
| RefSeq ORF | 1035 bp |
| Locus ID | 15485 |
| UniProt ID | P51656 |
| Cytogenetics | 11 64.17 cM |
| MW | 36.8 kDa |
| Summary | Favors the reduction of estrogens and androgens. Uses preferentially NADH.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_010475" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208711 | Hsd17b1 (untagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10ug) | 10 ug |
$457.00
|
|
| MG222239 | Hsd17b1 (tGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10ug) | 10 ug |
$657.00
|
|
| MR222239L3 | Lenti ORF clone of Hsd17b1 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) | 10 ug |
$757.00
|
|
| MR222239L4 | Lenti ORF clone of Hsd17b1 (mGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) | 10 ug |
$757.00
|
|
Citations
Development of potential preclinical candidates with promising in vitro ADME profile for the inhibition of type 1 and type 2 17β-Hydroxysteroid dehydrogenases: Design, synthesis, and biological evaluation
Abdelsamie, Salah, Siebenbürger et al
Eur J Med Chem (2019) 178, 93-107 DOI: 10.1016/j.ejmech.2019.05.084
Abdelsamie, Salah, Siebenbürger et al
Eur J Med Chem (2019) 178, 93-107 DOI: 10.1016/j.ejmech.2019.05.084
AI Summary: In this study, OriGene’s recombinant mouse 17β-HSD1 cDNA was used to transiently express the enzyme in HEK293 cells for evaluating the inhibitory effects of potential drug candidates.
Snippet: Human and mouse 17β-HSD2 preparations were obtained by isolating the microsomal fractions of human placenta and mouse liver homogenates, respectively, according to the described methods [44]. Incubations were run with [3H]-E2 (human: 500 nM;mouse: 10 nM), cofactor NAD+, and inhibitor. Human 17β-HSD1 was prepared from the cytosolic fractions of human placenta, whereas recombinant mouse 17β-HSD1 cDN...
Targeted Endocrine Therapy: Design, Synthesis, and Proof-of-Principle of 17β-Hydroxysteroid Dehydrogenase Type 2 Inhibitors in Bone Fracture Healing
Abdelsamie, Herath, Biskupek et al
J. Med. Chem. (2019)
Abdelsamie, Herath, Biskupek et al
J. Med. Chem. (2019)
AI Summary: In this study, OriGene's recombinant mouse 17β-HSD1 cDNA was used to transiently express the enzyme in HEK293 cells, enabling the evaluation of inhibitor specificity and activity in the development of targeted 17β-HSD2 inhibitors for bone fracture healing.
Snippet: Human and mouse 17β-HSD2 preparations were obtained by isolating the microsomal fractions of human placenta and mouse liver homogenates, respectively, according to the described methods.19 Incubations were run with [3 H]-E2 (human: 500 nM;mouse: 10 nM), cofactor NAD+ , and inhibitor. Human 17βHSD1 was prepared from the cytosolic fractions of human placenta, whereas recombinant mouse 17β-HSD1 cDNA ...
Highly Potent 17β-HSD2 Inhibitors with a Promising Pharmacokinetic Profile for Targeted Osteoporosis Therapy
Siebenbuerger, Hernandez-Olmos, Abdelsamie et al
J. Med. Chem. (2018)
Siebenbuerger, Hernandez-Olmos, Abdelsamie et al
J. Med. Chem. (2018)
AI Summary: OriGene's pCMV6-Entry vector containing the 17β-HSD1 (NM_010475) coding sequence was used to produce recombinant m17β-HSD1 enzyme in HEK293 cells for inhibitor testing in this study.
Snippet: … Recombinant m17β-HSD1 enzyme was produced by transfection of HEK293 cells with an 17β-HSD1 expression plasmid (coding sequence of NM_010475 in pCMV6Entry vector, OriGene Technologies, Inc.) according to a described procedure.33 Transfected cells were homogenized after 48 h by sonication (3 × 10 s) in a 40 mM Tris buffer containing 250 mM saccharose, 5 mM ethylenediaminetetraacetic acid (EDTA), 7 mM DTT, 1 mM phenylmethylsulfonyl fluoride, pH 7.5. …
17β-Hydroxysteroid Dehydrogenase Type 2 Inhibition: Discovery of Selective and Metabolically Stable Compounds Inhibiting Both the Human Enzyme and Its Murine Ortholog
Gargano, Allegretta, Perspicace et al
PLoS ONE (2015) 10 (7), e0134754 DOI: 10.1371/journal.pone.0134754
Gargano, Allegretta, Perspicace et al
PLoS ONE (2015) 10 (7), e0134754 DOI: 10.1371/journal.pone.0134754
Cited by 10
AI Summary: OriGene’s pCMV6-Entry m17β-HSD1 expression plasmid (NM_010475) was used to produce recombinant m17β-HSD1 enzyme in HEK 293 cells for inhibitor testing in this study.
Snippet: Recombinant m17β-HSD1 enzyme was produced by transfection of HEK 293 cells with a m17β-HSD1 expression plasmid (coding sequence of NM_010475 in pCMV6Entry vector, OriGene Technologies, Inc.) according to a described procedure[28]
Showing 1-4 of 4 papers.
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