Hsd17b1 (NM_010475) Mouse Untagged Clone

SKU
MC208711
Hsd17b1 (untagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10ug)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Hsd17b1
Synonyms 17beta-HSD; 17HSDB1; E2DH; Hsd17ba
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208711 representing NM_010475
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCCACGGTAGTGCTCATTACCGGTTGCTCCTCTGGAATCGGCATGCACTTGGCTGTTCGCCTAG
CTTCTGACCGTTCCCAGAGCTTCAAAGTTTATGCCACATTGCGGGACCTGAAGGCACAGGGACCATTGTT
GGAGGCCGCCAGGACTCAAGGATGCCCTCCTGGCTCCTTGGAGATACTGGAATTGGATGTCAGAGACTCC
AAATCTGTGGCTGCTGCCCAGGCATGCGTGACTGAGGGTCGTGTGGATGTACTGGTCTGTAATGCTGGCC
GCGGCTTGTTTGGGCCGCTAGAAGCACATGAATTGAACGCTGTGGGTGCTGTGTTGGATGTGAATGTGCT
TGGGACCATTCGGATGCTGCAGGCCTTTCTGCCAGACATGAAGAGGCGCCACTCTGGGCGTGTGCTGGTG
ACCGCGAGTGTGGGAGGCTTGATGGGACTGCCATTCCACGAAGTGTACTGTGCCAGCAAGTTTGCGCTCG
AAGGTTTGTGCGAGAGTCTGGCGATCCTGCTGCCGCTCTTTGGAGTCCATGTGAGCCTCATCGAGTGTGG
GGCAGTGCACACAGCCTTCTATGAAAAGCTGGTGGGCGGCCCCGGCGGGGCTCTGGAACGAGCAGATGCC
CAGACCCGCCACCTCTTTGCGCACTACCTGCGTGGTTATGAGCAAGCCCTGAGCGAGGCGCAGGACCCAG
AGGAGGTGACGGAGCTCTTCTTGACTGCAATGCGAGCCCCGCAGCCCGCGCTGCGCTACTTCTCCACGAA
CCGCTTCCTACCCCTGGCCCGAATGCGCACAGAGGATCCCAGTGGCAGCAGCTATGTCGCGGCCATGCAC
CAAGAAGCCTTCTCTAATCTGCAGACCCAGGAGAATGCCAAGGCTGGGGCCCAAGTCCCGGGGGTCTCGG
ACACGGCCTCCAGCGCCCTCATTTGCCTCCCTGAATGTGCAATCCCTAGGGTGGCATCTGAATTGGGGTG
GTCTGCATCGGATAAACCGGGTCAGGACAATTCATGTTACCAGCAAAAGATCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_010475
Insert Size 1035 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC125657, AAI25658
RefSeq Size 1317 bp
RefSeq ORF 1035 bp
Locus ID 15485
UniProt ID P51656
Cytogenetics 11 64.17 cM
Summary Favors the reduction of estrogens and androgens. Uses preferentially NADH.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010475" in other vectors (4)
SKU Description Size Price
MG222239 Hsd17b1 (tGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10ug) 10 ug
$657.00
MR222239 Hsd17b1 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) 10 ug
$289.00 $457.00
MR222239L3 Lenti ORF clone of Hsd17b1 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) 10 ug
$757.00
MR222239L4 Lenti ORF clone of Hsd17b1 (mGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) 10 ug
$757.00

file-alt Citations

Towards the evaluation in an animal disease model: Fluorinated 17β-HSD1 inhibitors showing strong activity towards both the human and the rat enzyme
Abdelsamie, Bey, Gargano et al
Eur J Med Chem (2015) 103, 56-68 DOI: 10.1016/j.ejmech.2015.08.030
Cited by 12
AI Summary: OriGene products contributed to this study by providing mouse and rat 17β-HSD1 expression plasmids, which were used to produce recombinant enzymes for inhibitor testing.
Snippet: Recombinant mouse and rat 17β-HSD1 enzymes were produced by transfection of HEK 293 cells with a mouse or rat 17β-HSD1 expression plasmid purchased from OriGene Technologies, Inc. (Rockville, USA)
Showing 1-1 of 1 papers.
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