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Hsd17b1 (NM_010475) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG222239
Hsd17b1 (tGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hsd17b1
Synonyms 17beta-HSD; 17HSDB1; E2DH; Hsd17ba
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222239 representing NM_010475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCCACGGTAGTGCTCATTACCGGTTGCTCCTCTGGAATCGGCATGCACTTGGCTGTTCGCCTAG
CTTCTGACCGTTCCCAGAGCTTCAAAGTTTATGCCACATTGCGGGACCTGAAGGCACAGGGACCATTGTT
GGAGGCCGCCAGGACTCAAGGATGCCCTCCTGGCTCCTTGGAGATACTGGAATTGGATGTCAGAGACTCC
AAATCTGTGGCTGCTGCCCAGGCATGCGTGACTGAGGGTCGTGTGGATGTACTGGTCTGTAATGCTGGCC
GCGGCTTGTTTGGGCCGCTAGAAGCACATGAATTGAACGCTGTGGGTGCTGTGTTGGATGTGAATGTGCT
TGGGACCATTCGGATGCTGCAGGCCTTTCTGCCAGACATGAAGAGGCGCCACTCTGGGCGTGTGCTGGTG
ACCGCGAGTGTGGGAGGCTTGATGGGACTGCCATTCCACGAAGTGTACTGTGCCAGCAAGTTTGCGCTCG
AAGGTTTGTGCGAGAGTCTGGCGATCCTGCTGCCGCTCTTTGGAGTCCATGTGAGCCTCATCGAGTGTGG
GGCAGTGCACACAGCCTTCTATGAAAAGCTGGTGGGCGGCCCCGGCGGGGCTCTGGAACGAGCAGATGCC
CAGACCCGCCACCTCTTTGCGCACTACCTGCGTGGTTATGAGCAAGCCCTGAGCGAGGCGCAGGACCCAG
AGGAGGTGACGGAGCTCTTCTTGACTGCAATGCGAGCCCCGCAGCCCGCGCTGCGCTACTTCTCCACGAA
CCGCTTCCTACCCCTGGCCCGAATGCGCACAGAGGATCCCAGTGGCAGCAGCTATGTCGCGGCCATGCAC
CAAGAAGCCTTCTCTAATCTGCAGACCCAGGAGAATGCCAAGGCTGGGGCCCAAGTCCCGGGGGTCTCGG
ACACGGCCTCCAGCGCCCTCATTTGCCTCCCTGAATGTGCAATCCCTAGGGTGGCATCTGAATTGGGGTG
GTCTGCATCGGATAAACCGGGTCAGGACAATTCATGTTACCAGCAAAAGATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222239 representing NM_010475
Red=Cloning site Green=Tags(s)

MDPTVVLITGCSSGIGMHLAVRLASDRSQSFKVYATLRDLKAQGPLLEAARTQGCPPGSLEILELDVRDS
KSVAAAQACVTEGRVDVLVCNAGRGLFGPLEAHELNAVGAVLDVNVLGTIRMLQAFLPDMKRRHSGRVLV
TASVGGLMGLPFHEVYCASKFALEGLCESLAILLPLFGVHVSLIECGAVHTAFYEKLVGGPGGALERADA
QTRHLFAHYLRGYEQALSEAQDPEEVTELFLTAMRAPQPALRYFSTNRFLPLARMRTEDPSGSSYVAAMH
QEAFSNLQTQENAKAGAQVPGVSDTASSALICLPECAIPRVASELGWSASDKPGQDNSCYQQKI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010475
ORF Size 1032 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010475.2
RefSeq Size 1317 bp
RefSeq ORF 1035 bp
Locus ID 15485
UniProt ID P51656
Cytogenetics 11 64.17 cM
Summary Favors the reduction of estrogens and androgens. Uses preferentially NADH.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010475" in other vectors (4)
SKU Description Size Price
MC208711 Hsd17b1 (untagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1), (10ug) 10 ug
$457.00
MR222239 Hsd17b1 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) 10 ug
$289.00 $457.00
MR222239L3 Lenti ORF clone of Hsd17b1 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) 10 ug
$757.00
MR222239L4 Lenti ORF clone of Hsd17b1 (mGFP-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 1 (Hsd17b1) 10 ug
$757.00

Plasmid Map for MG222239

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG222239
Circular map for MG222239

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