Phlpp2 (NM_001122594) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR219729
Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2)
$589.00
$1,159.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Phlpp2 |
| Synonyms | AI481772; C130044A18Rik; Phlppl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001122594 |
| ORF Size | 4065 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001122594.2, NP_001116066.2 |
| RefSeq Size | 7854 bp |
| RefSeq ORF | 4068 bp |
| Locus ID | 244650 |
| UniProt ID | Q8BXA7 |
| Cytogenetics | 8 D3 |
| MW | 150.1 kDa |
| Summary | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001122594" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC224335 | Phlpp2 (untagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), (10ug) | 10 ug |
$1,276.00
|
|
| MG219729 | Phlpp2 (tGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), (10ug) | 10 ug |
$1,359.00
|
|
| MR219729L3 | Lenti ORF clone of Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2) | 10 ug |
$1,459.00
|
|
| MR219729L4 | Lenti ORF clone of Phlpp2 (mGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2) | 10 ug |
$1,459.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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