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Phlpp2 (NM_001122594) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR219729L3
Lenti ORF clone of Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,459.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Phlpp2
Synonyms AI481772; C130044A18Rik; Phlppl
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR219729).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001122594
ORF Size 4065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001122594.2, NP_001116066.2
RefSeq Size 7854 bp
RefSeq ORF 4068 bp
Locus ID 244650
UniProt ID Q8BXA7
Cytogenetics 8 D3
Summary Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001122594" in other vectors (4)
SKU Description Size Price
MC224335 Phlpp2 (untagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), (10ug) 10 ug
$1,276.00
MG219729 Phlpp2 (tGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), (10ug) 10 ug
$1,359.00
MR219729 Phlpp2 (Myc-DDK-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2) 10 ug
$589.00 $1,159.00
MR219729L4 Lenti ORF clone of Phlpp2 (mGFP-tagged) - Mouse PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2) 10 ug
$1,459.00

Plasmid Map for MR219729L3

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Cloning scheme for MR219729L3
Circular map for MR219729L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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