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Gramd1b (BC070451) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210355
Gramd1b (Myc-DDK-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP
AAV Particle:
DDK
$589.00 $672.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gramd1b
Synonyms KIAA1201, mKIAA1201, 3222402H23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210355 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCCAGCACTGCCAGTAACTCCAACCGCAGCACGCCAGCCTGCTCTCCCATCCTCCGGAAGCGGT
CCCGCTCACCAACACCACAGAACCAGGACGGAGACACCATGGTGGAGAAGGGCTCAGATCACTCCTCGGA
CAAGTCCCCGTCCACCCCGGAGCAGGGTGTGCAACGCAGTTGCTCCTCACAGTCTGGTCGGAGCGGTGGT
AAAAATTCCAAGAAAAGCCAGAGTTGGTACAATGTGCTAAGCCCCACTTACAAGCAGAGAAATGAAGACT
TCAGAAAGCTCTTTAAGCAACTTCCAGACACGGAGCGCCTCATTGTTGATTACTCTTGTGCACTCCAAAG
AGACATTCTTCTTCAGGGCCGACTCTACCTCTCAGAAAATTGGATCTGCTTCTACAGCAACATCTTCCGC
TGGGAAACCCTGCTGACAGTCCGTTTGAAAGATATCTGCTCCATGACTAAAGAGAAAACAGCTCGCCTCA
TTCCCAATGCCATCCAAGTCTGCACTGATTCAGAAAAGCACTTTTTTACTTCCTTTGGAGCCCGGGACAG
GACGTACATGATGATGTTCCGGCTCTGGCAGAATGCTCTCCTTGAAAAGCCCCTATGTCCCAAGGAGCTC
TGGCACTTTGTCCACCAGTGCTACGGGAACGAGCTGGGCCTAACCAGTGATGATGAGGACTACGTGCCTC
CAGATGATGACTTCAACACAATGGGCTACTGTGAGGAGATCCCCATAGAAGAGAATGAAGTGAATGACAG
CTCATCCAAAAGTAGCATCGAGACCAAGCCGGATGCCAGCCCACAGCTGCCCAAGAAGTCCATTACCAAC
AGCACACTGACGTCTACGGGAAGCAGTGAAGCCCCTGTTTCGTTTGATGGCTTGCCATTGGAGGAGGAGG
TGATGGAGGGGGATGGTTCCCTAGAGAAGGAGCTTGCCATTGACAACATCATAGGGGAGAAGATCGAGAT
CATGGCGCCTGTGACCTCCCCTTCGCTGGACTTCAATGACAATGAGGATATCCCCACCGAGCTCAGTGAT
TCTTCAGATACCCACGATGAAGGAGAGGTCCAGGCCTTCTATGAGGACCTGAGTGGAAGACAATATGTGA
ATGAGGTCTTCAACTTCAGCGTGGACAAACTCTATGACCTGCTGTTCACCAACTCACCCTTCCTGCGGGA
TTTCATGGAGCAGCGGCGTTTCTCTGATATCATCTTCCATCCATGGAAAAAGGAGGAGAATGGAAACCAG
AGTCGAGTGATTCTCTACACTATCACCCTCACTAATCCTCTGGCTCCCAAAACAGCCACTGTCAGGGAGA
CACAGACCATGTACAAAGCGAGTCAGGAGAGCGAATGCTACGTGATAGATGCTGAAGTCCTCACCCACGA
TGTGCCGTACCATGACTACTTCTACACCATCAATCGCTACACACTCACCAGAGTGGCCCGGAACAAGAGC
AGACTCAGGGTCTCCACAGAGCTCCGCTATCGAAAACAGCCCTGGGGGTTTGTGAAAACGTTCATCGAGA
AGAACTTCTGGAGTGGACTGGAGGACTACTTCCGTCATTTAGAGACTGAACTAACCAAAACAGAGAGTAC
CTACTTGGCTGAGATACACAGGCAGTCACCCAAGGAGAAGGCTAGCAAGTCTTCAGCAGTCCGGAGGAGG
AAGCGTCCCCATGCCCACCTGCGGGTGCCTCACCTGGAAGAAGTGATGAGTCCTGTCACCACACCCACTG
ATGAAGATGTGGGCCACAGGATCAAGCACGTGGCAGGTTCCACGCAGACACGGCACATCCCGGAGGACAC
TCCTGATGGTTTTCACCTACAAAGTGTGTCCAAGCTGCTGCTGGTTATCAGCTGTGTGATCTGTTTCAGT
CTGGTGCTGCTGGTTGTCCTTAACATGATGCTCTTCTACAAGCTGTGGATGCTGGAATACACCACCCAGA
CCCTCACTGCCTGGCAGGGTCTCAGGCTCCAGGAAAGGTTACCCCAGTCTCAGACAGAATGGGCCCAGCT
ATTAGAATCCCAACAGAAGTACCACGACACCGAGCTCCAGAAGTGGAGGGAAATCATCAAATCCTCAGTG
TTGCTCCTGGACCAGATGAAGGACTCGCTTATCAATCTTCAGAATGGTATCAGGTCCCGAGACTACACAG
CTGAGAGCGACGAGAAGAGGAACCGCTATCAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210355 protein sequence
Red=Cloning site Green=Tags(s)

MASSTASNSNRSTPACSPILRKRSRSPTPQNQDGDTMVEKGSDHSSDKSPSTPEQGVQRSCSSQSGRSGG
KNSKKSQSWYNVLSPTYKQRNEDFRKLFKQLPDTERLIVDYSCALQRDILLQGRLYLSENWICFYSNIFR
WETLLTVRLKDICSMTKEKTARLIPNAIQVCTDSEKHFFTSFGARDRTYMMMFRLWQNALLEKPLCPKEL
WHFVHQCYGNELGLTSDDEDYVPPDDDFNTMGYCEEIPIEENEVNDSSSKSSIETKPDASPQLPKKSITN
STLTSTGSSEAPVSFDGLPLEEEVMEGDGSLEKELAIDNIIGEKIEIMAPVTSPSLDFNDNEDIPTELSD
SSDTHDEGEVQAFYEDLSGRQYVNEVFNFSVDKLYDLLFTNSPFLRDFMEQRRFSDIIFHPWKKEENGNQ
SRVILYTITLTNPLAPKTATVRETQTMYKASQESECYVIDAEVLTHDVPYHDYFYTINRYTLTRVARNKS
RLRVSTELRYRKQPWGFVKTFIEKNFWSGLEDYFRHLETELTKTESTYLAEIHRQSPKEKASKSSAVRRR
KRPHAHLRVPHLEEVMSPVTTPTDEDVGHRIKHVAGSTQTRHIPEDTPDGFHLQSVSKLLLVISCVICFS
LVLLVVLNMMLFYKLWMLEYTTQTLTAWQGLRLQERLPQSQTEWAQLLESQQKYHDTELQKWREIIKSSV
LLLDQMKDSLINLQNGIRSRDYTAESDEKRNRYH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC070451
ORF Size 2202 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC070451, AAH70451
RefSeq Size 7363 bp
RefSeq ORF 2204 bp
Locus ID 235283
Cytogenetics 9 A5.1
MW 84.8 kDa
Summary Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461).UniProtKB/Swiss-Prot Function
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"BC070451" in other vectors (4)
SKU Description Size Price
MC220920 Gramd1b (untagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509), (10ug) 10 ug
$740.00
MG210355 Gramd1b (tGFP-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) 10 ug
$872.00
MR210355L1 Lenti ORF clone of Gramd1b (Myc-DDK-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) 10 ug
$972.00
MR210355L2 Lenti ORF clone of Gramd1b (mGFP-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) 10 ug
$972.00

Plasmid Map for MR210355

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210355
Circular map for MR210355

file-alt Citations

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