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Gramd1b (BC070451) Mouse Untagged Clone

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SKU
MC220920
Gramd1b (untagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $740.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gramd1b
Synonyms KIAA1201, mKIAA1201, 3222402H23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC070451
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCCAGCACTGCCAGTAACTCCAACCGCAGCACGCCAGCCTGCTCTCCCATCCTCCGGAAGCGGT
CCCGCTCACCAACACCACAGAACCAGGACGGAGACACCATGGTGGAGAAGGGCTCAGATCACTCCTCGGA
CAAGTCCCCGTCCACCCCGGAGCAGGGTGTGCAACGCAGTTGCTCCTCACAGTCTGGTCGGAGCGGTGGT
AAAAATTCCAAGAAAAGCCAGAGTTGGTACAATGTGCTAAGCCCCACTTACAAGCAGAGAAATGAAGACT
TCAGAAAGCTCTTTAAGCAACTTCCAGACACGGAGCGCCTCATTGTTGATTACTCTTGTGCACTCCAAAG
AGACATTCTTCTTCAGGGCCGACTCTACCTCTCAGAAAATTGGATCTGCTTCTACAGCAACATCTTCCGC
TGGGAAACCCTGCTGACAGTCCGTTTGAAAGATATCTGCTCCATGACTAAAGAGAAAACAGCTCGCCTCA
TTCCCAATGCCATCCAAGTCTGCACTGATTCAGAAAAGCACTTTTTTACTTCCTTTGGAGCCCGGGACAG
GACGTACATGATGATGTTCCGGCTCTGGCAGAATGCTCTCCTTGAAAAGCCCCTATGTCCCAAGGAGCTC
TGGCACTTTGTCCACCAGTGCTACGGGAACGAGCTGGGCCTAACCAGTGATGATGAGGACTACGTGCCTC
CAGATGATGACTTCAACACAATGGGCTACTGTGAGGAGATCCCCATAGAAGAGAATGAAGTGAATGACAG
CTCATCCAAAAGTAGCATCGAGACCAAGCCGGATGCCAGCCCACAGCTGCCCAAGAAGTCCATTACCAAC
AGCACACTGACGTCTACGGGAAGCAGTGAAGCCCCTGTTTCGTTTGATGGCTTGCCATTGGAGGAGGAGG
TGATGGAGGGGGATGGTTCCCTAGAGAAGGAGCTTGCCATTGACAACATCATAGGGGAGAAGATCGAGAT
CATGGCGCCTGTGACCTCCCCTTCGCTGGACTTCAATGACAATGAGGATATCCCCACCGAGCTCAGTGAT
TCTTCAGATACCCACGATGAAGGAGAGGTCCAGGCCTTCTATGAGGACCTGAGTGGAAGACAATATGTGA
ATGAGGTCTTCAACTTCAGCGTGGACAAACTCTATGACCTGCTGTTCACCAACTCACCCTTCCTGCGGGA
TTTCATGGAGCAGCGGCGTTTCTCTGATATCATCTTCCATCCATGGAAAAAGGAGGAGAATGGAAACCAG
AGTCGAGTGATTCTCTACACTATCACCCTCACTAATCCTCTGGCTCCCAAAACAGCCACTGTCAGGGAGA
CACAGACCATGTACAAAGCGAGTCAGGAGAGCGAATGCTACGTGATAGATGCTGAAGTCCTCACCCACGA
TGTGCCGTACCATGACTACTTCTACACCATCAATCGCTACACACTCACCAGAGTGGCCCGGAACAAGAGC
AGACTCAGGGTCTCCACAGAGCTCCGCTATCGAAAACAGCCCTGGGGGTTTGTGAAAACGTTCATCGAGA
AGAACTTCTGGAGTGGACTGGAGGACTACTTCCGTCATTTAGAGACTGAACTAACCAAAACAGAGAGTAC
CTACTTGGCTGAGATACACAGGCAGTCACCCAAGGAGAAGGCTAGCAAGTCTTCAGCAGTCCGGAGGAGG
AAGCGTCCCCATGCCCACCTGCGGGTGCCTCACCTGGAAGAAGTGATGAGTCCTGTCACCACACCCACTG
ATGAAGATGTGGGCCACAGGATCAAGCACGTGGCAGGTTCCACGCAGACACGGCACATCCCGGAGGACAC
TCCTGATGGTTTTCACCTACAAAGTGTGTCCAAGCTGCTGCTGGTTATCAGCTGTGTGATCTGTTTCAGT
CTGGTGCTGCTGGTTGTCCTTAACATGATGCTCTTCTACAAGCTGTGGATGCTGGAATACACCACCCAGA
CCCTCACTGCCTGGCAGGGTCTCAGGCTCCAGGAAAGGTTACCCCAGTCTCAGACAGAATGGGCCCAGCT
ATTAGAATCCCAACAGAAGTACCACGACACCGAGCTCCAGAAGTGGAGGGAAATCATCAAATCCTCAGTG
TTGCTCCTGGACCAGATGAAGGACTCGCTTATCAATCTTCAGAATGGTATCAGGTCCCGAGACTACACAG
CTGAGAGCGACGAGAAGAGGAACCGCTATCATTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC070451
Insert Size 2205 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC070451, AAH70451
RefSeq Size 7363 bp
RefSeq ORF 2204 bp
Locus ID 235283
Cytogenetics 9 A5.1
Summary Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis in the adrenal gland and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461).UniProtKB/Swiss-Prot Function
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"BC070451" in other vectors (4)
SKU Description Size Price
MG210355 Gramd1b (tGFP-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) 10 ug
$872.00
MR210355 Gramd1b (Myc-DDK-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) 10 ug
$589.00 $672.00
MR210355L1 Lenti ORF clone of Gramd1b (Myc-DDK-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) 10 ug
$972.00
MR210355L2 Lenti ORF clone of Gramd1b (mGFP-tagged) - Mouse GRAM domain containing 1B (cDNA clone MGC:99432 IMAGE:30616509) 10 ug
$972.00

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