Hsf1 (NM_008296) Mouse Tagged ORF Clone
$289.00
$468.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hsf1 |
| Synonyms | AA960185; Hsf1alpha; Hsf1beta; HSTF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008296 |
| ORF Size | 1509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008296.3 |
| RefSeq Size | 2018 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 15499 |
| UniProt ID | P38532 |
| Cytogenetics | 15 35.95 cM |
| MW | 55.4 kDa |
| Summary | Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008296" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC200965 | Hsf1 (untagged) - Mouse heat shock factor 1 (Hsf1), (10ug) | 10 ug |
$469.00
|
|
| MG208087 | Hsf1 (tGFP-tagged) - Mouse heat shock factor 1 (Hsf1) | 10 ug |
$489.00
$668.00
|
|
| MR208087L1 | Lenti ORF clone of Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) | 10 ug |
$768.00
|
|
| MR208087L2 | Lenti ORF clone of Hsf1 (mGFP-tagged) - Mouse heat shock factor 1 (Hsf1) | 10 ug |
$768.00
|
|
| MR208087L3 | Lenti ORF clone of Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) | 10 ug |
$768.00
|
|
| MR208087L4 | Lenti ORF clone of Hsf1 (mGFP-tagged) - Mouse heat shock factor 1 (Hsf1) | 10 ug |
$768.00
|
|
Citations
RBM4-mediated intron excision of Hsf1 induces BDNF for cerebellar foliation
Shen, Tsai, Chou et al
Commun Biol (2024) 7 (1), 1712 DOI: 10.1038/s42003-024-07328-6
Shen, Tsai, Chou et al
Commun Biol (2024) 7 (1), 1712 DOI: 10.1038/s42003-024-07328-6
Cited by 4
AI Summary: In this study, the HSF1 expression plasmid (OriGene, MR208087) was used to investigate the role of HSF1 in BDNF expression and cerebellar development.
Snippet: cDNA3.1-based vectors for expression of FLAG-tagged RBM4 (human RBM4A gene), RBM4-SA mutant, PTBP1, and PTBP2 have been previously described14,15,34,35. The HSF1 expression plasmid was purchased from Origene (MR208087). The pLKO.1-shluc-puro-based Rbm4 or Hsf1 shRNA expression vectors were obtained from the National RNAi core facility in Academia Sinica. To generate the Hsf1 minigene, a genomic DN
Activation of TrkB signaling mitigates cerebellar anomalies caused by Rbm4-Bdnf deficiency
Tsai, Shen, D et al
Commun Biol (2023) 6 (1), 910 DOI: 10.1038/s42003-023-05294-z
Tsai, Shen, D et al
Commun Biol (2023) 6 (1), 910 DOI: 10.1038/s42003-023-05294-z
Cited by 5
AI Summary: OriGene products contributed to this study by providing pCMV6-Entry based vectors (empty, PS100001; Nsmf, MR221711; Hsf1, MR208087), which were used to assess BDNF gene expression and investigate the molecular mechanisms underlying cerebellar anomalies in Rbm4-deficient mice.
Snippet: Rbm4 knockdown GC (see above) using primers (Supplementary Table 1). BDNF gene expression assay Three pCMV6-Entry based vectors (empty, PS100001;Nsmf, MR221711;Hsf1, MR208087) were obtained from Origene. NSMF(∆E6) was generated by deleting exon 6 using a PCR-based method;the sequence was verified by Sanger sequencing. Rbm4 was depleted in P7 GCs at DIV1 by transient transfection of pLKO.1
Decreased ZO1 expression causes loss of time-dependent tight junction function in the liver of ob/ob mice
Tsurudome, Morita, Horiguchi et al
Research Square (2021)
Tsurudome, Morita, Horiguchi et al
Research Square (2021)
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
