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Hsf1 (NM_008296) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208087
Hsf1 (tGFP-tagged) - Mouse heat shock factor 1 (Hsf1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $668.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hsf1
Synonyms AA960185; Hsf1alpha; Hsf1beta; HSTF
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208087 representing NM_008296
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTGGCCGTGGGCCCCGGTGCAGCGGGGCCCAGCAACGTCCCGGCCTTCCTAACCAAGCTGTGGA
CCCTCGTGAGCGACCCGGACACAGACGCGCTCATCTGCTGGAGCCCGAGTGGGAACAGCTTCCACGTGTT
TGACCAGGGCCAGTTTGCCAAGGAGGTGCTGCCCAAGTACTTCAAGCACAACAACATGGCTAGCTTCGTG
CGGCAGCTCAACATGTATGGCTTCCGAAAAGTAGTCCACATTGAGCAGGGTGGCCTGGTCAAGCCTGAGA
GAGATGACACCGAGTTCCAGCATCCTTGTTTCTTGCGTGGACAGGAACAGCTCCTTGAGAACATCAAGAG
GAAAGTGACCAGCGTGTCCACCCTGAAGAGTGAGGACATAAAAATACGCCAGGACAGTGTCACCCGGCTG
TTGACAGATGTGCAGCTGATGAAGGGGAAACAGGAGTGTATGGACTCCAAGCTCCTGGCCATGAAGCACG
AGAACGAGGCCCTGTGGCGGGAGGTGGCCAGCCTTCGGCAGAAGCATGCCCAGCAGCAAAAAGTTGTCAA
CAAGCTCATTCAGTTCCTGATCTCACTGGTGCAGTCGAACCGGATCCTGGGGGTGAAGAGAAAGATCCCT
CTGATGTTGAGTGACAGCAACTCAGCACACTCTGTGCCCAAGTATGGTCGACAGTACTCCCTGGAGCATG
TCCATGGTCCTGGCCCATACTCAGCTCCATCTCCAGCCTACAGCAGCTCTAGCCTTTACTCCTCTGATGC
TGTCACCAGCTCTGGACCCATAATCTCCGATATCACTGAGCTGGCTCCCACCAGCCCTTTGGCCTCCCCA
GGCAGGAGCATAGATGAGAGGCCTCTGTCCAGCAGCACTCTGGTCCGTGTCAAGCAAGAGCCCCCCAGCC
CACCTCACAGCCCTCGGGTACTGGAGGCGAGCCCTGGGCGCCCATCCTCCATGGATACCCCTTTGTCCCC
AACTGCCTTCATTGACTCCATCCTTCGAGAGAGCGAGCCTACCCCTGCTGCCTCAAACACAGCCCCTATG
GACACAACCGGAGCCCAAGCCCCCGCACTCCCGACCCCCTCCACCCCTGAGAAGTGCCTCAGCGTAGCCT
GCCTAGACAAGAACGAGCTAAGTGATCACCTGGATGCCATGGACTCCAACCTGGACAACCTGCAGACCAT
GCTGACAAGCCACGGCTTCAGTGTGGACACCAGTGCCCTGCTGGACATTCAGGAGCTTCTGTCTCCACAA
GAGCCTCCCAGGCCTATTGAGGCAGAGAACAGTAACCCCGACTCAGGAAAGCAGCTGGTGCACTACACGG
CTCAGCCTCTGTTCCTGCTGGATCCTGATGCTGTGGACACAGGGAGCAGTGAGCTGCCTGTGCTCTTTGA
GCTGGGGGAGAGCTCCTACTTCTCTGAGGGGGATGACTACACGGATGATCCCACCATCTCTCTTCTGACA
GGCACTGAACCCCATAAAGCCAAGGACCCCACTGTCTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208087 representing NM_008296
Red=Cloning site Green=Tags(s)

MDLAVGPGAAGPSNVPAFLTKLWTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFV
RQLNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSVSTLKSEDIKIRQDSVTRL
LTDVQLMKGKQECMDSKLLAMKHENEALWREVASLRQKHAQQQKVVNKLIQFLISLVQSNRILGVKRKIP
LMLSDSNSAHSVPKYGRQYSLEHVHGPGPYSAPSPAYSSSSLYSSDAVTSSGPIISDITELAPTSPLASP
GRSIDERPLSSSTLVRVKQEPPSPPHSPRVLEASPGRPSSMDTPLSPTAFIDSILRESEPTPAASNTAPM
DTTGAQAPALPTPSTPEKCLSVACLDKNELSDHLDAMDSNLDNLQTMLTSHGFSVDTSALLDIQELLSPQ
EPPRPIEAENSNPDSGKQLVHYTAQPLFLLDPDAVDTGSSELPVLFELGESSYFSEGDDYTDDPTISLLT
GTEPHKAKDPTVS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008296
ORF Size 1509 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008296.3
RefSeq Size 2018 bp
RefSeq ORF 1512 bp
Locus ID 15499
UniProt ID P38532
Cytogenetics 15 35.95 cM
Summary Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner.UniProtKB/Swiss-Prot Function
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"NM_008296" in other vectors (6)
SKU Description Size Price
MC200965 Hsf1 (untagged) - Mouse heat shock factor 1 (Hsf1), (10ug) 10 ug
$469.00
MR208087 Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) 10 ug
$289.00 $468.00
MR208087L1 Lenti ORF clone of Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) 10 ug
$768.00
MR208087L2 Lenti ORF clone of Hsf1 (mGFP-tagged) - Mouse heat shock factor 1 (Hsf1) 10 ug
$768.00
MR208087L3 Lenti ORF clone of Hsf1 (Myc-DDK-tagged) - Mouse heat shock factor 1 (Hsf1) 10 ug
$768.00
MR208087L4 Lenti ORF clone of Hsf1 (mGFP-tagged) - Mouse heat shock factor 1 (Hsf1) 10 ug
$768.00

Plasmid Map for MG208087

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208087
Circular map for MG208087

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