OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Fezf2 (NM_080433) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR207270
Fezf2 (Myc-DDK-tagged) - Mouse Fez family zinc finger 2 (Fezf2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Fezf2 or NM_080433"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fezf2
Synonyms AI451466; AI852056; Fez; Fezl; Zfp312
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR207270 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCTCAGCTTCCCTGGAGACCATGGTGCCCCCGGCCTGCCCGCGCGCTGGAGCGTCACCGGCCA
CTTCTAAAACACTAGCTTTCTCTATCGAGCGCATCATGGCCAAGACGTCCGAGCCCCGAGCGCCTTTCGA
GCCCCGGCCTGCTGCGTTAGAGGCAGACAGCAGCCAGAGCAAGAAACTGCTCAACCTCTGCTCGCCGCTG
CCCTGTATGATCCCTCTCCAGCCTCTAGGCTACGAGGTGCCGTCCAAGACGCTGCTCAGTTACTCGGAGT
TCTGGAAGAGCAGCCTCCGGGCGGGCGGCGGTGGAGGAGGAGGCAGCGGCGGGGGGGCCCCAGTGTGCGG
CGCCAGCGGCTTGTGCAAAACCAACTGTGGCGTGTGCTGCAAGGCCGAACTGGGCCTCGCGCCTTCTGCG
CTGCCCGCCGGCAGGGTCATCAAACCGCAGGTCATCAACCAGGCTGTGGGGCTGCCTGCCAGCGGCTCTC
TCTACTACTTCAACTACCTGGACTCCACCGCTTACCCACCTTCGGAGCTCCTCGGAGGCCACCTTTTCCC
ATCCGGCCTCCTCAACGCACAGGCCCCCACTTCCCTGGCTGCTCACCCCAAGCTTTTTCTGCTGGAGAAC
GCCAAACTGGCCAGCCTGGCTGCGGACAAGTTCCCCCACCCAGCTTCCTATCCCCATAAGGAGCGCTTGC
ATGCGCCACTGGAGCAGGTGCTGAAGGAGAACTCGGCCTTGACAGCTGAACGAGGGGGAGTCAAGAGCCA
CAGCAAACTACCGGGGGGCTCTACTGACAGCAAACCCAAAAACTTCACCTGCGAAGTGTGCGGCAAGGTG
TTCAATGCTCACTATAACCTCACCCGCCACATGCCTGTCCACACCGGAGCTAGACCGTTTGTGTGCAAAG
TCTGTGGCAAAGGCTTCCGCCAGGCCAGCACTCTCTGCAGACACAAAATTATCCATACCCAGGAAAAACC
ACATAAGTGTAACCAGTGCGGCAAAGCCTTCAATCGCAGCTCCACGCTCAACACGCACATCCGCATCCAC
GCGGGCTACAAGCCCTTCGTCTGCGAGTTTTGTGGCAAAGGCTTTCACCAAAAAGGGAACTACAAGAATC
ACAAGCTCACCCACAGCGGCGAGAAGCAGTACAAATGCACTATCTGTAACAAGGCCTTCCATCAGGTCTA
CAATCTGACCTTCCACATGCACACCCACAACGACAAGAAGCCTTTCACGTGTGCCACTTGCGGCAAAGGT
TTTTGCAGAAACTTTGACTTAAAGAAACATGTGCGCAAACTTCATGACAGCGTGGGTCCCACCGCCACCC
CCTCAGCAAAGGACCTAGCCAGGACAGTTCAGAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR207270 protein sequence
Red=Cloning site Green=Tags(s)

MASSASLETMVPPACPRAGASPATSKTLAFSIERIMAKTSEPRAPFEPRPAALEADSSQSKKLLNLCSPL
PCMIPLQPLGYEVPSKTLLSYSEFWKSSLRAGGGGGGGSGGGAPVCGASGLCKTNCGVCCKAELGLAPSA
LPAGRVIKPQVINQAVGLPASGSLYYFNYLDSTAYPPSELLGGHLFPSGLLNAQAPTSLAAHPKLFLLEN
AKLASLAADKFPHPASYPHKERLHAPLEQVLKENSALTAERGGVKSHSKLPGGSTDSKPKNFTCEVCGKV
FNAHYNLTRHMPVHTGARPFVCKVCGKGFRQASTLCRHKIIHTQEKPHKCNQCGKAFNRSSTLNTHIRIH
AGYKPFVCEFCGKGFHQKGNYKNHKLTHSGEKQYKCTICNKAFHQVYNLTFHMHTHNDKKPFTCATCGKG
FCRNFDLKKHVRKLHDSVGPTATPSAKDLARTVQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080433
ORF Size 1365 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080433.3
RefSeq Size 2304 bp
RefSeq ORF 1368 bp
Locus ID 54713
UniProt ID Q9ESP5
Cytogenetics 14 A1
MW 48.9 kDa
Summary Transcription repressor. Required for the specification of corticospinal motor neurons and other subcerebral projection neurons. May play a role in layer and neuronal subtype-specific patterning of subcortical projections and axonal fasciculation. Controls the development of dendritic arborization and spines of large layer V pyramidal neurons. Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_080433" in other vectors (4)
SKU Description Size Price
MC202571 Fezf2 (untagged) - Mouse Fez family zinc finger 2 (Fezf2), (10ug) 10 ug
$457.00
MG207270 Fezf2 (tGFP-tagged) - Mouse Fez family zinc finger 2 (Fezf2) 10 ug
$657.00
MR207270L3 Lenti ORF clone of Fezf2 (Myc-DDK-tagged) - Mouse Fez family zinc finger 2 (Fezf2) 10 ug
$757.00
MR207270L4 Lenti ORF clone of Fezf2 (mGFP-tagged) - Mouse Fez family zinc finger 2 (Fezf2) 10 ug
$757.00

Plasmid Map for MR207270

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR207270
Circular map for MR207270

file-alt Citations

Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture
Lattke, Goldstone, Guillemot
bioRxiv (2020)
Cited by 1
Snippet: Rorb, Dbx2, Lhx2, and Fezf2 open reading frame (ORF) constructs from Origene (MR225771, MR215327, MR206390, MR207270) were re-cloned into a modified version of the TetO-FUWEGFP lentiviral expression plasmid (Addgene #30130, (Vierbuchen et al., 2010)). … We then replaced Nr1d1 with Rorb, Dbx2, Lhx2 or Fezf2 to generate V5-tagged doxycycline-inducible lentiviral expression vectors for these transcription factors
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products