Fezf2 (NM_080433) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG207270
Fezf2 (tGFP-tagged) - Mouse Fez family zinc finger 2 (Fezf2)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fezf2
Synonyms AI451466; AI852056; Fez; Fezl; Zfp312
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207270 representing NM_080433
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCTCAGCTTCCCTGGAGACCATGGTGCCCCCGGCCTGCCCGCGCGCTGGAGCGTCACCGGCCA
CTTCTAAAACACTAGCTTTCTCTATCGAGCGCATCATGGCCAAGACGTCCGAGCCCCGAGCGCCTTTCGA
GCCCCGGCCTGCTGCGTTAGAGGCAGACAGCAGCCAGAGCAAGAAACTGCTCAACCTCTGCTCGCCGCTG
CCCTGTATGATCCCTCTCCAGCCTCTAGGCTACGAGGTGCCGTCCAAGACGCTGCTCAGTTACTCGGAGT
TCTGGAAGAGCAGCCTCCGGGCGGGCGGCGGTGGAGGAGGAGGCAGCGGCGGGGGGGCCCCAGTGTGCGG
CGCCAGCGGCTTGTGCAAAACCAACTGTGGCGTGTGCTGCAAGGCCGAACTGGGCCTCGCGCCTTCTGCG
CTGCCCGCCGGCAGGGTCATCAAACCGCAGGTCATCAACCAGGCTGTGGGGCTGCCTGCCAGCGGCTCTC
TCTACTACTTCAACTACCTGGACTCCACCGCTTACCCACCTTCGGAGCTCCTCGGAGGCCACCTTTTCCC
ATCCGGCCTCCTCAACGCACAGGCCCCCACTTCCCTGGCTGCTCACCCCAAGCTTTTTCTGCTGGAGAAC
GCCAAACTGGCCAGCCTGGCTGCGGACAAGTTCCCCCACCCAGCTTCCTATCCCCATAAGGAGCGCTTGC
ATGCGCCACTGGAGCAGGTGCTGAAGGAGAACTCGGCCTTGACAGCTGAACGAGGGGGAGTCAAGAGCCA
CAGCAAACTACCGGGGGGCTCTACTGACAGCAAACCCAAAAACTTCACCTGCGAAGTGTGCGGCAAGGTG
TTCAATGCTCACTATAACCTCACCCGCCACATGCCTGTCCACACCGGAGCTAGACCGTTTGTGTGCAAAG
TCTGTGGCAAAGGCTTCCGCCAGGCCAGCACTCTCTGCAGACACAAAATTATCCATACCCAGGAAAAACC
ACATAAGTGTAACCAGTGCGGCAAAGCCTTCAATCGCAGCTCCACGCTCAACACGCACATCCGCATCCAC
GCGGGCTACAAGCCCTTCGTCTGCGAGTTTTGTGGCAAAGGCTTTCACCAAAAAGGGAACTACAAGAATC
ACAAGCTCACCCACAGCGGCGAGAAGCAGTACAAATGCACTATCTGTAACAAGGCCTTCCATCAGGTCTA
CAATCTGACCTTCCACATGCACACCCACAACGACAAGAAGCCTTTCACGTGTGCCACTTGCGGCAAAGGT
TTTTGCAGAAACTTTGACTTAAAGAAACATGTGCGCAAACTTCATGACAGCGTGGGTCCCACCGCCACCC
CCTCAGCAAAGGACCTAGCCAGGACAGTTCAGAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207270 representing NM_080433
Red=Cloning site Green=Tags(s)

MASSASLETMVPPACPRAGASPATSKTLAFSIERIMAKTSEPRAPFEPRPAALEADSSQSKKLLNLCSPL
PCMIPLQPLGYEVPSKTLLSYSEFWKSSLRAGGGGGGGSGGGAPVCGASGLCKTNCGVCCKAELGLAPSA
LPAGRVIKPQVINQAVGLPASGSLYYFNYLDSTAYPPSELLGGHLFPSGLLNAQAPTSLAAHPKLFLLEN
AKLASLAADKFPHPASYPHKERLHAPLEQVLKENSALTAERGGVKSHSKLPGGSTDSKPKNFTCEVCGKV
FNAHYNLTRHMPVHTGARPFVCKVCGKGFRQASTLCRHKIIHTQEKPHKCNQCGKAFNRSSTLNTHIRIH
AGYKPFVCEFCGKGFHQKGNYKNHKLTHSGEKQYKCTICNKAFHQVYNLTFHMHTHNDKKPFTCATCGKG
FCRNFDLKKHVRKLHDSVGPTATPSAKDLARTVQS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080433
ORF Size 1365 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080433.3
RefSeq Size 2058 bp
RefSeq ORF 1368 bp
Locus ID 54713
UniProt ID Q9ESP5
Cytogenetics 14 A1
Summary Transcription repressor. Required for the specification of corticospinal motor neurons and other subcerebral projection neurons. May play a role in layer and neuronal subtype-specific patterning of subcortical projections and axonal fasciculation. Controls the development of dendritic arborization and spines of large layer V pyramidal neurons. Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_080433" in other vectors (4)
SKU Description Size Price
MC202571 Fezf2 (untagged) - Mouse Fez family zinc finger 2 (Fezf2), (10ug) 10 ug
$457.00
MR207270 Fezf2 (Myc-DDK-tagged) - Mouse Fez family zinc finger 2 (Fezf2) 10 ug
$289.00 $457.00
MR207270L3 Lenti ORF clone of Fezf2 (Myc-DDK-tagged) - Mouse Fez family zinc finger 2 (Fezf2) 10 ug
$757.00
MR207270L4 Lenti ORF clone of Fezf2 (mGFP-tagged) - Mouse Fez family zinc finger 2 (Fezf2) 10 ug
$757.00

Plasmid Map for MG207270

Download sequence for

Cloning scheme for MG207270

Circular map for MG207270

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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