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RNF70 (PJA1) (NM_145119) Human Tagged ORF Clone

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SKU
RC224490
PJA1 (Myc-DDK-tagged)-Human praja ring finger 1 (PJA1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $598.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF70
Synonyms PRAJA1; RNF70
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC224490 representing NM_145119
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCAGGAATCTAGCAAGCCTGTATGGCCCAATCCAACAGGAGGGTATCAGTCCAATACAGGTAGGA
GGTATGGAAGAAGGCATGCTTATGTCAGTTTCAGGCCACCCACGAGCCAGCGGGAAAGGATTGCCAGCCA
GAGAAAGACGAACTCCGAAGTCCCAATGCACAGATCAGCCCCCAGTCAAACCACCAAGAGGAGCCGATCG
CCATTTTCCACTACTCGTCGTAGTTGGGACGACAGCGAGAGTTCGGGAACCAACCTGAATATTGATAATG
AGGACTATTCCAGGTATCCGCCAAGAGAGTACAGAGCTTCGGGTAGCAGAAGAGGAATGGCCTATGGACA
TATTGACTCTTATGGGGCAGATGATAGTGAGGAGGAGGGGGCTGGGCCTGTTGAGCGACCGCCAGTGAGA
GGGAAAACTGGCAAGTTTAAAGATGATAAGCTGTATGACCCAGAGAAAGGGGCAAGGTCTTTGGCTGGGC
CACCTCCACATTTCTCTAGTTTTAGCCGTGATGTGAGAGAGGAGCGAGACAAGTTAGACCCAGTCCCTGC
AGCAAGATGCTCAGCTAGCAGAGCTGACTTCCTGCCACAAAGTAGTGTGGCCTCACAGTCGTCTTCTGAA
GGCAAGCTGGCTACAAAAGGTGACAGCTCGGAGAGGGAGAGAAGGGAGCAAAATTTACCTGCACGTCCCA
GCAGGGCTCCTGTGAGTATTTGTGGTGGTGGGGAAAACACCTCAAAGAGTGCAGAGGAACCTGTGGTCAG
GCCCAAAATCAGAAACCTGGCAAGTCCAAACTGCGTGAAACCAAAAATTTTTTTTGATACTGATGATGAT
GACGATATGCCACACAGTACTTCCAGGTGGAGGGATACCGCCAATGACAATGAGGGCCACTCGGATGGCC
TGGCAAGAAGAGGGAGAGGCGAGAGTTCAAGTGGCTATCCCGAGCCAAAGTACCCTGAAGACAAACGGGA
AGCGAGGAGTGACCAAGTGAAACCAGAAAAGGTGCCGAGACGACGACGCACCATGGCCGACCCTGACTTC
TGGACGCACAGTGATGATTACTACAAATACTGCGACGAAGACTCTGACAGTGACAAAGAGTGGATTGCTG
CTCTGCGTCGGAAATATCGAAGCCGAGAGCAAACCCTGTCCTCCAGTGGCGAAAGCTGGGAGACTCTGCC
GGGGAAAGAAGAGCGGGAACCTCCACAGGCTAAGGTGAGTGCCAGCACTGGCACCAGCCCTGGCCCCGGT
GCTAGTGCCAGTGCCGGGGCTGGCGCCGGGGCCAGTGCTGGCAGCAATGGCAGCAATTACCTTGAAGAAG
TTCGAGAACCATCTCTTCAGGAAGAGCAGGCATCCCTGGAAGAAGGAGAAATTCCTTGGCTCCAGTACCA
TGAGAATGACAGTAGCAGTGAGGGGGATAATGATTCTGGTCACGAGTTGATGCAACCTGGGGTATTCATG
CTGGATGGAAACAACAACCTTGAAGATGACTCCAGTGTGAGCGAAGACCTAGAAGTGGATTGGAGCCTCT
TTGATGGATTTGCAGATGGGTTAGGAGTGGCTGAAGCCATTTCCTATGTGGACCCTCAGTTCCTCACCTA
CATGGCACTTGAAGAACGCCTGGCCCAGGCAATGGAAACTGCCCTTGCGCACTTGGAGTCTCTCGCAGTG
GATGTAGAGGTGGCCAATCCACCAGCAAGCAAGGAGAGCATTGACGCTCTTCCCGAGATCCTGGTCACTG
AAGATCATGGCGCAGTTGGTCAGGAGATGTGCTGCCCCATCTGCTGTAGCGAATATGTGAAGGGGGAGGT
GGCAACTGAGCTGCCGTGCCACCACTATTTCCACAAGCCGTGTGTGTCCATCTGGCTTCAGAAGTCAGGC
ACCTGCCCCGTGTGCCGCTGCATGTTCCCTCCCCCACTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC224490 representing NM_145119
Red=Cloning site Green=Tags(s)

MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRS
PFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVR
GKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSE
GKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDD
DDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDF
WTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPG
ASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFM
LDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAV
DVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSG
TCPVCRCMFPPPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145119
ORF Size 1929 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145119.4
RefSeq Size 2700 bp
RefSeq ORF 1932 bp
Locus ID 64219
UniProt ID Q8NG27
Cytogenetics Xq13.1
Protein Families Druggable Genome
MW 70.8 kDa
Summary This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2010
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"NM_145119" in other vectors (6)
SKU Description Size Price
RC224490L1 Lenti ORF clone of Human praja ring finger 1 (PJA1), transcript variant 1, Myc-DDK-tagged 10 ug
$898.00
RC224490L2 Lenti ORF clone of Human praja ring finger 1 (PJA1), transcript variant 1, mGFP tagged 10 ug
$898.00
RC224490L3 Lenti ORF clone of Human praja ring finger 1 (PJA1), transcript variant 1, Myc-DDK-tagged 10 ug
$898.00
RC224490L4 Lenti ORF clone of Human praja ring finger 1 (PJA1), transcript variant 1, mGFP tagged 10 ug
$898.00
RG224490 PJA1 (tGFP-tagged) - Human praja ring finger 1 (PJA1), transcript variant 1 10 ug
$489.00 $798.00
SC124584 PJA1 (untagged)-Human praja ring finger 1 (PJA1), transcript variant 1 10 ug
$599.00

Plasmid Map for RC224490

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC224490
Circular map for RC224490

file-alt Citations

Targeting Methylglyoxal Metabolism to Enhance Ferroptosis Sensitivity in Tumor Therapy
Zhang, Han, Wang et al
Adv Sci (Weinh) (2025)
Cited by 2
AI Summary: OriGene products contributed to this study by providing the wild-type PJA1 (RC224490) and GLO1 (RC203826) plasmids, which were used as templates to generate specific mutant constructs for investigating the role of the PJA1-GLO1 axis in ferroptosis sensitivity in cancer cells.
Snippet: To generate the human PJA1 C598A, GLO1 K47R/K48R, and GLO1 K157R/K159R mutations, a site-directed mutagenesis approach was employed. Plasmids containing the wild-type PJA1 (OriGene, RC224490) and GLO1 (OriGene, RC203826) coding sequences served as templates for the mutagenesis reactions
Showing 1-1 of 1 papers.
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